ENSG00000170448

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000381538 ENSG00000170448 HEK293_OSMI2_2hA HEK293_TMG_2hB NFXL1 protein_coding protein_coding 7.276395 2.388919 10.53457 0.1683956 0.2014916 2.136043 3.1350767 1.17899842 4.8221709 0.27973212 0.4678514881 2.022925 0.43827917 0.48853333 0.4567000 -0.03183333 9.270329e-01 1.946694e-14 FALSE TRUE
ENST00000502448 ENSG00000170448 HEK293_OSMI2_2hA HEK293_TMG_2hB NFXL1 protein_coding retained_intron 7.276395 2.388919 10.53457 0.1683956 0.2014916 2.136043 0.5630479 0.78622301 0.0000000 0.17797200 0.0000000000 -6.315101 0.15907917 0.33476667 0.0000000 -0.33476667 5.901355e-12 1.946694e-14 FALSE TRUE
ENST00000507131 ENSG00000170448 HEK293_OSMI2_2hA HEK293_TMG_2hB NFXL1 protein_coding retained_intron 7.276395 2.388919 10.53457 0.1683956 0.2014916 2.136043 1.7103475 0.00000000 3.2189976 0.00000000 0.2355972951 8.334943 0.15698333 0.00000000 0.3065667 0.30656667 1.946694e-14 1.946694e-14 FALSE TRUE
ENST00000507489 ENSG00000170448 HEK293_OSMI2_2hA HEK293_TMG_2hB NFXL1 protein_coding protein_coding 7.276395 2.388919 10.53457 0.1683956 0.2014916 2.136043 0.8027242 0.31401362 1.6223241 0.13548193 0.0001095964 2.332801 0.10666250 0.12896667 0.1541000 0.02513333 7.824765e-01 1.946694e-14 FALSE TRUE
ENST00000511452 ENSG00000170448 HEK293_OSMI2_2hA HEK293_TMG_2hB NFXL1 protein_coding retained_intron 7.276395 2.388919 10.53457 0.1683956 0.2014916 2.136043 0.7114860 0.09413037 0.6920635 0.04931644 0.1106877110 2.753210 0.09937083 0.04136667 0.0654000 0.02403333 6.209452e-01 1.946694e-14   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000170448 E001 0.1515154 0.0430608869 1.000000e+00   4 47847233 47847239 7 - 0.061 0.001 -6.886
ENSG00000170448 E002 121.9545331 0.0055325896 1.212321e-09 1.767648e-08 4 47847240 47848303 1064 - 1.919 2.209 0.972
ENSG00000170448 E003 26.8921698 0.0013932531 8.897100e-01 9.335059e-01 4 47848304 47848336 33 - 1.349 1.372 0.082
ENSG00000170448 E004 0.0000000       4 47851057 47851082 26 -      
ENSG00000170448 E005 0.0000000       4 47851083 47851094 12 -      
ENSG00000170448 E006 30.2306513 0.0007111482 8.142507e-01 8.822974e-01 4 47851095 47851148 54 - 1.404 1.398 -0.021
ENSG00000170448 E007 42.2324241 0.0005515941 8.129441e-02 1.615764e-01 4 47851856 47851942 87 - 1.514 1.637 0.417
ENSG00000170448 E008 3.5848171 0.0045330022 6.516468e-01 7.624668e-01 4 47851943 47852208 266 - 0.569 0.656 0.383
ENSG00000170448 E009 39.3187208 0.0005785251 9.362557e-01 9.639005e-01 4 47855059 47855163 105 - 1.506 1.524 0.062
ENSG00000170448 E010 35.1638606 0.0005900318 5.852409e-01 7.089736e-01 4 47862846 47862915 70 - 1.455 1.506 0.175
ENSG00000170448 E011 55.2751964 0.0004608133 2.620001e-01 3.997202e-01 4 47875127 47875293 167 - 1.639 1.714 0.257
ENSG00000170448 E012 0.3332198 0.0291482091 2.708302e-01   4 47877012 47877191 180 - 0.061 0.232 2.231
ENSG00000170448 E013 46.6963316 0.0007117703 2.452856e-01 3.803697e-01 4 47878525 47878665 141 - 1.567 1.651 0.285
ENSG00000170448 E014 0.0000000       4 47878666 47878704 39 -      
ENSG00000170448 E015 21.1073015 0.0009421687 8.548658e-01 9.102249e-01 4 47879096 47879117 22 - 1.258 1.253 -0.017
ENSG00000170448 E016 36.6745211 0.0038193845 4.406877e-01 5.823629e-01 4 47884346 47884437 92 - 1.492 1.445 -0.161
ENSG00000170448 E017 56.3865721 0.0046756639 3.694720e-03 1.228075e-02 4 47885498 47885657 160 - 1.698 1.507 -0.653
ENSG00000170448 E018 51.3339838 0.0005086773 3.216287e-02 7.617116e-02 4 47885879 47885999 121 - 1.649 1.524 -0.425
ENSG00000170448 E019 41.9779065 0.0005703245 6.400382e-01 7.533648e-01 4 47890613 47890703 91 - 1.543 1.524 -0.065
ENSG00000170448 E020 48.5004684 0.0005111250 7.864635e-01 8.632530e-01 4 47894180 47894302 123 - 1.604 1.600 -0.016
ENSG00000170448 E021 51.2963295 0.0004888944 1.829164e-01 3.048532e-01 4 47896523 47896647 125 - 1.639 1.568 -0.241
ENSG00000170448 E022 49.3379447 0.0005462711 2.592955e-01 3.966888e-01 4 47897967 47898081 115 - 1.619 1.560 -0.202
ENSG00000170448 E023 41.1174039 0.0010075107 9.815340e-01 9.925203e-01 4 47898757 47898857 101 - 1.528 1.542 0.048
ENSG00000170448 E024 52.4716312 0.0005705097 1.676345e-01 2.851130e-01 4 47898959 47899120 162 - 1.649 1.576 -0.248
ENSG00000170448 E025 50.0818144 0.0004963106 2.073245e-01 3.352939e-01 4 47899370 47899548 179 - 1.626 1.560 -0.228
ENSG00000170448 E026 36.2211824 0.0112061853 6.732594e-01 7.789890e-01 4 47903193 47903323 131 - 1.477 1.460 -0.060
ENSG00000170448 E027 36.8953917 0.0094685846 4.805812e-01 6.188391e-01 4 47905237 47905346 110 - 1.492 1.447 -0.157
ENSG00000170448 E028 35.3164817 0.0009255500 4.821282e-03 1.543374e-02 4 47910824 47910994 171 - 1.502 1.287 -0.747
ENSG00000170448 E029 30.7871216 0.0006864749 2.926929e-02 7.052258e-02 4 47913969 47914205 237 - 1.441 1.270 -0.593
ENSG00000170448 E030 9.2367447 0.0018083318 6.460307e-01 7.580587e-01 4 47914365 47914549 185 - 0.906 0.976 0.262
ENSG00000170448 E031 9.7067101 0.0017202411 1.204548e-01 2.206154e-01 4 47914550 47914612 63 - 0.981 0.773 -0.794
ENSG00000170448 E032 0.2944980 0.4020548637 1.000000e+00   4 47914613 47914667 55 - 0.114 0.001 -7.878