ENSG00000170421

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000549176 ENSG00000170421 HEK293_OSMI2_2hA HEK293_TMG_2hB KRT8 protein_coding retained_intron 32.68898 66.21475 19.82233 1.60108 0.5808216 -1.739517 0.9617419 0.6174846 1.3903041 0.1886913 0.19083165 1.158088 0.0450000 0.00940000 0.06973333 0.06033333 4.609406e-06 4.609406e-06 FALSE  
ENST00000692008 ENSG00000170421 HEK293_OSMI2_2hA HEK293_TMG_2hB KRT8 protein_coding protein_coding 32.68898 66.21475 19.82233 1.60108 0.5808216 -1.739517 27.0839997 53.8227218 17.0184679 2.3993540 0.07313086 -1.660535 0.8472375 0.81206667 0.86006667 0.04800000 4.880356e-01 4.609406e-06 FALSE  
MSTRG.7330.9 ENSG00000170421 HEK293_OSMI2_2hA HEK293_TMG_2hB KRT8 protein_coding   32.68898 66.21475 19.82233 1.60108 0.5808216 -1.739517 2.0697050 5.6714630 0.2287685 0.5140152 0.22876850 -4.572578 0.0379500 0.08613333 0.01093333 -0.07520000 6.064073e-02 4.609406e-06 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000170421 E001 0.2214452 0.0384187892 1.0000000000   12 52897187 52897190 4 - 0.000 0.065 7.635
ENSG00000170421 E002 1.1834139 0.0116472451 0.1741245739 0.2935264066 12 52897191 52897192 2 - 0.471 0.217 -1.593
ENSG00000170421 E003 4.8973997 0.0035656628 0.5242628050 0.6578507757 12 52897193 52897193 1 - 0.746 0.644 -0.422
ENSG00000170421 E004 12.8925076 0.0017325895 0.7308327555 0.8226427954 12 52897194 52897207 14 - 1.059 1.018 -0.149
ENSG00000170421 E005 17.4920732 0.0033267597 0.9966610514 1.0000000000 12 52897208 52897215 8 - 1.148 1.146 -0.006
ENSG00000170421 E006 19.3218649 0.0052948665 0.9975070379 1.0000000000 12 52897216 52897222 7 - 1.186 1.185 -0.004
ENSG00000170421 E007 81.5552475 0.0020163786 0.7548460309 0.8403556210 12 52897223 52897280 58 - 1.781 1.797 0.054
ENSG00000170421 E008 87.3044915 0.0004293159 0.5774462412 0.7027079020 12 52897281 52897296 16 - 1.804 1.829 0.086
ENSG00000170421 E009 427.4356738 0.0001578841 0.0030321483 0.0103609429 12 52897297 52897618 322 - 2.454 2.518 0.214
ENSG00000170421 E010 125.2571823 0.0002607984 0.0184413093 0.0481020340 12 52898461 52898468 8 - 1.892 1.990 0.329
ENSG00000170421 E011 226.7421562 0.0001822227 0.0002821868 0.0013072365 12 52898469 52898519 51 - 2.136 2.249 0.378
ENSG00000170421 E012 2.2570555 0.0131037604 0.1783049209 0.2990168220 12 52898520 52898678 159 - 0.630 0.390 -1.164
ENSG00000170421 E013 448.1153151 0.0007035828 0.0001481725 0.0007391148 12 52898679 52898899 221 - 2.451 2.543 0.306
ENSG00000170421 E014 233.0262194 0.0002028991 0.0352144143 0.0820523355 12 52899775 52899793 19 - 2.192 2.255 0.208
ENSG00000170421 E015 481.0825350 0.0001425372 0.0695108181 0.1425452572 12 52899794 52899934 141 - 2.528 2.563 0.119
ENSG00000170421 E016 329.8308898 0.0002343727 0.6948088128 0.7955411622 12 52899935 52899995 61 - 2.404 2.393 -0.039
ENSG00000170421 E017 216.5238604 0.0002195104 0.2300932620 0.3625801901 12 52899996 52900007 12 - 2.245 2.207 -0.127
ENSG00000170421 E018 271.0922347 0.0002166747 0.3781749030 0.5224691221 12 52900008 52900065 58 - 2.333 2.307 -0.086
ENSG00000170421 E019 358.0093719 0.0001901995 0.0199654506 0.0514121886 12 52900588 52900683 96 - 2.477 2.421 -0.186
ENSG00000170421 E020 1.5049038 0.0093789839 0.4826947960 0.6208252839 12 52900684 52901158 475 - 0.218 0.361 0.990
ENSG00000170421 E021 308.7797883 0.0001688869 0.0008905867 0.0035811557 12 52901159 52901219 61 - 2.435 2.352 -0.278
ENSG00000170421 E022 1.1791475 0.0139218451 0.1748199477 0.2944654192 12 52901220 52901863 644 - 0.471 0.217 -1.592
ENSG00000170421 E023 462.9105870 0.0001304229 0.0035235873 0.0117944446 12 52901864 52902072 209 - 2.591 2.531 -0.201
ENSG00000170421 E024 3.7362256 0.0044426111 0.0270678384 0.0661299482 12 52902073 52902204 132 - 0.837 0.493 -1.477
ENSG00000170421 E025 4.2350491 0.0038952100 0.0241621627 0.0601431582 12 52902887 52902966 80 - 0.218 0.675 2.515
ENSG00000170421 E026 2.0303989 0.0072548858 0.2832160554 0.4233091286 12 52903492 52903648 157 - 0.558 0.361 -1.008
ENSG00000170421 E027 0.1482932 0.0411730798 1.0000000000   12 52904208 52904250 43 - 0.000 0.065 9.276
ENSG00000170421 E028 166.3512965 0.0002170771 0.6116440129 0.7307304731 12 52904658 52904675 18 - 2.115 2.095 -0.065
ENSG00000170421 E029 408.0304091 0.0014419711 0.0005724436 0.0024306913 12 52904676 52905027 352 - 2.562 2.469 -0.310
ENSG00000170421 E030 103.1284578 0.0034036756 0.0181050513 0.0473573066 12 52905028 52905079 52 - 1.987 1.867 -0.402
ENSG00000170421 E031 1.6951159 0.0081842542 0.4562883303 0.5967310183 12 52905132 52905210 79 - 0.471 0.330 -0.786
ENSG00000170421 E032 0.0000000       12 52906521 52906618 98 -      
ENSG00000170421 E033 0.0000000       12 52906648 52907060 413 -      
ENSG00000170421 E034 0.0000000       12 52926412 52926469 58 -      
ENSG00000170421 E035 3.0235121 0.0057718981 0.7245036372 0.8180021846 12 52946614 52948483 1870 - 0.471 0.535 0.312
ENSG00000170421 E036 1.0360631 0.0825184818 0.2432345523 0.3779749184 12 52948484 52948650 167 - 0.000 0.294 11.586
ENSG00000170421 E037 10.3689407 0.0296579406 0.0123864745 0.0344628760 12 52948651 52948828 178 - 0.558 1.000 1.784
ENSG00000170421 E038 0.6986918 0.0167053234 0.8009911666 0.8731787757 12 52949216 52949455 240 - 0.218 0.172 -0.422
ENSG00000170421 E039 15.1712377 0.0012619367 0.0083688136 0.0247100563 12 52949456 52949517 62 - 0.794 1.130 1.259
ENSG00000170421 E040 8.1382002 0.0022523253 0.0426684185 0.0959374756 12 52949518 52949578 61 - 0.558 0.884 1.351
ENSG00000170421 E041 2.1839009 0.0890624052 0.2911106851 0.4319403564 12 52949579 52949585 7 - 0.218 0.465 1.553
ENSG00000170421 E042 5.5197408 0.0029197577 0.1211138611 0.2215291255 12 52949586 52949714 129 - 0.471 0.744 1.215
ENSG00000170421 E043 19.1711396 0.0009497911 0.0126548763 0.0350993843 12 52949715 52949954 240 - 0.946 1.224 1.007