ENSG00000170365

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000302085 ENSG00000170365 HEK293_OSMI2_2hA HEK293_TMG_2hB SMAD1 protein_coding protein_coding 7.73903 5.216068 10.18299 0.9033121 0.503236 0.9637795 3.138612 1.351813 6.524766 0.3756183 0.7231941 2.262607 0.3822417 0.25066667 0.6473333 0.3966667 4.747572e-03 1.209517e-19 FALSE TRUE
ENST00000394092 ENSG00000170365 HEK293_OSMI2_2hA HEK293_TMG_2hB SMAD1 protein_coding protein_coding 7.73903 5.216068 10.18299 0.9033121 0.503236 0.9637795 1.645565 3.440770 0.000000 0.1284630 0.0000000 -8.430774 0.2774542 0.68776667 0.0000000 -0.6877667 1.209517e-19 1.209517e-19 FALSE TRUE
MSTRG.25540.4 ENSG00000170365 HEK293_OSMI2_2hA HEK293_TMG_2hB SMAD1 protein_coding   7.73903 5.216068 10.18299 0.9033121 0.503236 0.9637795 1.839441 0.356563 2.550056 0.3565630 0.7737295 2.804042 0.2338292 0.05083333 0.2471667 0.1963333 1.480913e-01 1.209517e-19 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000170365 E001 0.0000000       4 145481194 145481305 112 +      
ENSG00000170365 E002 0.1515154 0.0443448028 8.670592e-01   4 145481306 145481312 7 + 0.093 0.000 -10.354
ENSG00000170365 E003 0.1515154 0.0443448028 8.670592e-01   4 145481313 145481314 2 + 0.093 0.000 -11.860
ENSG00000170365 E004 0.1472490 0.0443012807 8.672166e-01   4 145481315 145481400 86 + 0.093 0.000 -11.860
ENSG00000170365 E005 0.4417591 0.0283354518 3.132982e-01 4.558633e-01 4 145481401 145481664 264 + 0.093 0.274 1.876
ENSG00000170365 E006 0.5922303 0.0178646779 6.219525e-01 7.388340e-01 4 145481665 145481697 33 + 0.235 0.159 -0.706
ENSG00000170365 E007 1.0372116 0.0115835163 6.303125e-01 7.454888e-01 4 145481698 145481744 47 + 0.342 0.275 -0.444
ENSG00000170365 E008 0.1472490 0.0443012807 8.672166e-01   4 145481745 145481752 8 + 0.093 0.000 -11.860
ENSG00000170365 E009 6.0516816 0.0026297793 1.047127e-01 1.976855e-01 4 145481853 145481870 18 + 0.914 0.733 -0.706
ENSG00000170365 E010 15.5662640 0.0012900425 1.679256e-03 6.216389e-03 4 145481871 145482038 168 + 1.300 1.047 -0.901
ENSG00000170365 E011 0.4375944 0.0266657784 2.142022e-01 3.434875e-01 4 145482486 145482497 12 + 0.235 0.000 -13.503
ENSG00000170365 E012 0.7320925 0.0595593913 7.630147e-02 1.535831e-01 4 145482498 145482707 210 + 0.342 0.000 -13.976
ENSG00000170365 E013 0.5911862 0.0184107690 1.170327e-01 2.157201e-01 4 145482708 145482791 84 + 0.292 0.000 -13.955
ENSG00000170365 E014 0.7728905 0.0154466983 4.214341e-01 5.645873e-01 4 145482792 145482804 13 + 0.292 0.159 -1.120
ENSG00000170365 E015 0.6277178 0.0197616390 6.278739e-01 7.435463e-01 4 145482805 145482810 6 + 0.235 0.159 -0.705
ENSG00000170365 E016 0.4804688 0.0223912206 9.598521e-01 9.787477e-01 4 145482811 145482812 2 + 0.170 0.159 -0.120
ENSG00000170365 E017 0.4804688 0.0223912206 9.598521e-01 9.787477e-01 4 145482813 145482820 8 + 0.170 0.159 -0.120
ENSG00000170365 E018 0.0000000       4 145482821 145482868 48 +      
ENSG00000170365 E019 0.3332198 0.0308510316 6.933834e-01   4 145482869 145483073 205 + 0.093 0.159 0.880
ENSG00000170365 E020 0.2966881 0.0273633744 4.082979e-01   4 145483074 145483176 103 + 0.170 0.000 -12.949
ENSG00000170365 E021 0.0000000       4 145484513 145484764 252 +      
ENSG00000170365 E022 0.0000000       4 145503852 145503979 128 +      
ENSG00000170365 E023 0.0000000       4 145513241 145513378 138 +      
ENSG00000170365 E024 76.6412697 0.0004315015 3.050918e-15 1.084404e-13 4 145514438 145514799 362 + 1.979 1.675 -1.024
ENSG00000170365 E025 67.5560902 0.0007926302 1.270383e-04 6.457997e-04 4 145514800 145515013 214 + 1.878 1.742 -0.459
ENSG00000170365 E026 0.0000000       4 145525720 145525815 96 +      
ENSG00000170365 E027 77.3190568 0.0023005510 4.927272e-03 1.572754e-02 4 145539804 145540061 258 + 1.925 1.836 -0.300
ENSG00000170365 E028 52.7836208 0.0004636678 2.355123e-01 3.689803e-01 4 145542582 145542698 117 + 1.738 1.717 -0.071
ENSG00000170365 E029 1.8673596 0.0620873629 8.348527e-01 8.966209e-01 4 145544178 145546076 1899 + 0.465 0.442 -0.120
ENSG00000170365 E030 0.4418608 0.0275575180 2.141737e-01 3.434535e-01 4 145546077 145546171 95 + 0.235 0.000 -13.496
ENSG00000170365 E031 0.5953508 0.0191350643 6.127585e-01 7.316336e-01 4 145546172 145546519 348 + 0.170 0.274 0.877
ENSG00000170365 E032 0.4407149 0.0217681645 9.554334e-01 9.760363e-01 4 145546520 145546702 183 + 0.170 0.159 -0.122
ENSG00000170365 E033 79.1539631 0.0004519977 7.130250e-03 2.156329e-02 4 145546703 145546924 222 + 1.928 1.857 -0.238
ENSG00000170365 E034 89.9814669 0.0004706022 9.016820e-01 9.413541e-01 4 145553784 145554040 257 + 1.943 1.973 0.104
ENSG00000170365 E035 284.2124089 0.0023179235 1.331265e-22 1.135543e-20 4 145557791 145559176 1386 + 2.335 2.584 0.828