ENSG00000170312

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000395284 ENSG00000170312 HEK293_OSMI2_2hA HEK293_TMG_2hB CDK1 protein_coding protein_coding 83.47393 25.94911 156.309 4.817396 6.315467 2.59018 62.19060 23.325820 119.13415 4.2823127 2.4222864 2.352090 0.7757875 0.89970000 0.76360000 -0.1361 4.783137e-07 4.584854e-08 FALSE TRUE
ENST00000487784 ENSG00000170312 HEK293_OSMI2_2hA HEK293_TMG_2hB CDK1 protein_coding retained_intron 83.47393 25.94911 156.309 4.817396 6.315467 2.59018 4.85508 1.041279 10.22913 0.3367047 0.6722475 3.283876 0.0546375 0.03823333 0.06543333 0.0272 4.356892e-02 4.584854e-08   FALSE
ENST00000519078 ENSG00000170312 HEK293_OSMI2_2hA HEK293_TMG_2hB CDK1 protein_coding protein_coding 83.47393 25.94911 156.309 4.817396 6.315467 2.59018 8.42522 0.000000 11.32599 0.0000000 4.2866906 10.146694 0.0782500 0.00000000 0.07050000 0.0705 4.584854e-08 4.584854e-08 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000170312 E001 0.5181333 2.104176e-02 4.643945e-01 6.041165e-01 10 60778331 60778364 34 + 0.111 0.238 1.323
ENSG00000170312 E002 2.2016871 1.804273e-01 8.879129e-01 9.323433e-01 10 60778365 60778453 89 + 0.438 0.396 -0.224
ENSG00000170312 E003 1.9865846 8.341991e-03 3.309542e-01 4.745232e-01 10 60778454 60778456 3 + 0.439 0.238 -1.263
ENSG00000170312 E004 2.6058834 2.383380e-02 5.684482e-01 6.951427e-01 10 60778457 60778459 3 + 0.503 0.391 -0.582
ENSG00000170312 E005 7.3938913 1.773154e-02 1.052369e-03 4.140566e-03 10 60778460 60778477 18 + 0.910 0.237 -3.292
ENSG00000170312 E006 65.9498940 6.966129e-03 1.313840e-04 6.649474e-04 10 60778478 60778492 15 + 1.769 1.488 -0.956
ENSG00000170312 E007 373.2390570 7.803353e-03 4.552680e-03 1.468876e-02 10 60778493 60778570 78 + 2.497 2.353 -0.482
ENSG00000170312 E008 21.7774783 6.903349e-03 7.881408e-01 8.643229e-01 10 60778571 60778668 98 + 1.266 1.249 -0.062
ENSG00000170312 E009 345.6509927 6.983078e-03 1.192037e-02 3.334385e-02 10 60780141 60780152 12 + 2.461 2.337 -0.413
ENSG00000170312 E010 453.4890792 2.730707e-03 1.154681e-04 5.930460e-04 10 60780153 60780202 50 + 2.579 2.454 -0.419
ENSG00000170312 E011 1.0308569 2.222681e-02 2.363614e-01 3.699434e-01 10 60783494 60783593 100 + 0.305 0.001 -9.456
ENSG00000170312 E012 808.9420023 1.327316e-04 2.398086e-06 1.833547e-05 10 60784705 60784861 157 + 2.821 2.757 -0.214
ENSG00000170312 E013 698.2996526 1.057921e-03 5.849658e-01 7.087620e-01 10 60785664 60785787 124 + 2.741 2.763 0.071
ENSG00000170312 E014 116.4321532 1.333372e-03 1.263373e-18 6.873702e-17 10 60785788 60787069 1282 + 2.032 1.537 -1.675
ENSG00000170312 E015 66.7614632 1.906429e-03 2.481016e-03 8.707156e-03 10 60787671 60788059 389 + 1.764 1.588 -0.597
ENSG00000170312 E016 436.4766826 6.058014e-04 3.981584e-01 5.419070e-01 10 60788060 60788083 24 + 2.536 2.570 0.112
ENSG00000170312 E017 798.0537551 2.703448e-04 3.942153e-01 5.380866e-01 10 60788084 60788230 147 + 2.805 2.804 -0.003
ENSG00000170312 E018 4.7258517 4.468567e-02 9.042691e-01 9.430659e-01 10 60788231 60788234 4 + 0.681 0.665 -0.066
ENSG00000170312 E019 305.5972414 7.378193e-04 2.855523e-01 4.258905e-01 10 60791890 60791893 4 + 2.381 2.426 0.152
ENSG00000170312 E020 946.5639162 7.958195e-05 2.173992e-01 3.472503e-01 10 60791894 60792053 160 + 2.872 2.904 0.105
ENSG00000170312 E021 893.9671549 1.601422e-04 1.664983e-01 2.836595e-01 10 60792148 60792289 142 + 2.846 2.882 0.117
ENSG00000170312 E022 1220.5705811 2.126548e-03 1.796260e-13 4.929684e-12 10 60793877 60794852 976 + 2.948 3.123 0.584