ENSG00000170185

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000307017 ENSG00000170185 HEK293_OSMI2_2hA HEK293_TMG_2hB USP38 protein_coding protein_coding 8.213926 2.688605 14.77162 0.2975164 0.4360679 2.453519 3.0160956 1.5442915 6.126692 0.1338033 0.2848555 1.981204 0.38658750 0.57873333 0.4144 -0.16433333 1.048664e-02 7.850313e-11 FALSE TRUE
ENST00000510377 ENSG00000170185 HEK293_OSMI2_2hA HEK293_TMG_2hB USP38 protein_coding protein_coding 8.213926 2.688605 14.77162 0.2975164 0.4360679 2.453519 4.5396137 0.3705655 8.355432 0.0500271 0.2515855 4.458223 0.45670417 0.14473333 0.5660 0.42126667 7.850313e-11 7.850313e-11 FALSE TRUE
ENST00000682469 ENSG00000170185 HEK293_OSMI2_2hA HEK293_TMG_2hB USP38 protein_coding protein_coding 8.213926 2.688605 14.77162 0.2975164 0.4360679 2.453519 0.2820164 0.2248470 0.000000 0.2248470 0.0000000 -4.553649 0.05481250 0.09316667 0.0000 -0.09316667 5.379444e-01 7.850313e-11 FALSE TRUE
ENST00000682486 ENSG00000170185 HEK293_OSMI2_2hA HEK293_TMG_2hB USP38 protein_coding retained_intron 8.213926 2.688605 14.77162 0.2975164 0.4360679 2.453519 0.2058921 0.5427391 0.000000 0.3567267 0.0000000 -5.788527 0.07685833 0.18146667 0.0000 -0.18146667 3.479005e-02 7.850313e-11 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000170185 E001 10.6280226 0.1027156114 2.138812e-01 3.431064e-01 4 143184917 143184966 50 + 1.011 0.785 -0.860
ENSG00000170185 E002 64.7175225 0.0004136335 5.695969e-04 2.419960e-03 4 143184967 143185428 462 + 1.753 1.589 -0.557
ENSG00000170185 E003 28.8544663 0.0159329346 5.249873e-02 1.136083e-01 4 143185429 143185450 22 + 1.415 1.254 -0.560
ENSG00000170185 E004 91.3880935 0.0002973122 9.277961e-05 4.881094e-04 4 143185451 143185693 243 + 1.900 1.747 -0.516
ENSG00000170185 E005 132.4101611 0.0034259116 2.767781e-05 1.659644e-04 4 143185694 143186088 395 + 2.060 1.888 -0.578
ENSG00000170185 E006 40.2713337 0.0101522247 6.925730e-01 7.937824e-01 4 143186089 143186132 44 + 1.522 1.520 -0.005
ENSG00000170185 E007 59.8618933 0.0004107460 1.530828e-01 2.658747e-01 4 143187826 143187935 110 + 1.699 1.655 -0.150
ENSG00000170185 E008 40.5364631 0.0005672900 4.853895e-01 6.232629e-01 4 143187936 143187961 26 + 1.529 1.519 -0.034
ENSG00000170185 E009 68.4314407 0.0003620062 1.207352e-02 3.371102e-02 4 143195716 143195845 130 + 1.768 1.668 -0.338
ENSG00000170185 E010 63.8529869 0.0004251941 1.368071e-02 3.749558e-02 4 143197823 143197924 102 + 1.737 1.634 -0.351
ENSG00000170185 E011 85.9347012 0.0013566547 9.406030e-05 4.940376e-04 4 143203408 143203566 159 + 1.874 1.701 -0.586
ENSG00000170185 E012 0.1515154 0.0435626919 1.000000e+00   4 143204417 143204532 116 + 0.060 0.000 -7.867
ENSG00000170185 E013 101.0674609 0.0008201452 9.162433e-03 2.668894e-02 4 143206033 143206226 194 + 1.930 1.846 -0.283
ENSG00000170185 E014 2.6041859 0.0347262965 4.713343e-01 6.104619e-01 4 143206227 143207489 1263 + 0.509 0.385 -0.645
ENSG00000170185 E015 0.5891098 0.0182905891 4.807435e-01 6.189711e-01 4 143208158 143209512 1355 + 0.203 0.000 -10.185
ENSG00000170185 E016 0.1451727 0.0435472289 1.000000e+00   4 143209513 143209563 51 + 0.060 0.000 -7.867
ENSG00000170185 E017 81.2541042 0.0003378937 1.879930e-01 3.111853e-01 4 143209564 143209657 94 + 1.826 1.799 -0.089
ENSG00000170185 E018 91.6934705 0.0003489707 1.259460e-03 4.841445e-03 4 143212318 143212424 107 + 1.896 1.779 -0.394
ENSG00000170185 E019 440.8309820 0.0002104117 2.287314e-01 3.609296e-01 4 143213581 143214943 1363 + 2.539 2.600 0.203
ENSG00000170185 E020 3.3697015 0.0046604327 9.620998e-01 9.800984e-01 4 143214944 143215577 634 + 0.565 0.586 0.092
ENSG00000170185 E021 327.2396379 0.0035289084 8.786477e-24 8.505732e-22 4 143220295 143223874 3580 + 2.331 2.670 1.129