ENSG00000170153

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000420921 ENSG00000170153 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF150 protein_coding protein_coding 2.834424 1.751814 2.952741 0.1237114 0.1062269 0.7498711 0.10172855 0.00000000 0.2275122 0.00000000 0.11376862 4.5699299 0.0281750 0.00000000 0.07973333 0.07973333 0.282395104 0.001502075 FALSE  
ENST00000515057 ENSG00000170153 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF150 protein_coding processed_transcript 2.834424 1.751814 2.952741 0.1237114 0.1062269 0.7498711 0.04435582 0.09950328 0.0000000 0.01641215 0.00000000 -3.4529021 0.0197625 0.05826667 0.00000000 -0.05826667 0.001502075 0.001502075 FALSE  
ENST00000515673 ENSG00000170153 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF150 protein_coding protein_coding 2.834424 1.751814 2.952741 0.1237114 0.1062269 0.7498711 2.39280599 1.61121866 2.4530716 0.14321748 0.05797353 0.6033799 0.8682833 0.91716667 0.83210000 -0.08506667 0.214438191 0.001502075 FALSE  
MSTRG.25523.5 ENSG00000170153 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF150 protein_coding   2.834424 1.751814 2.952741 0.1237114 0.1062269 0.7498711 0.19614734 0.00000000 0.2074717 0.00000000 0.20747169 4.4427557 0.0488250 0.00000000 0.06596667 0.06596667 0.893369273 0.001502075 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000170153 E001 369.8061769 0.0029049577 7.527925e-17 3.289075e-15 4 140859807 140865570 5764 - 2.479 2.661 0.607
ENSG00000170153 E002 1.2480608 0.0101980291 8.144472e-01 8.824296e-01 4 140865571 140865575 5 - 0.362 0.347 -0.091
ENSG00000170153 E003 118.4554774 0.0031211860 2.580259e-09 3.543989e-08 4 140865576 140868044 2469 - 2.147 1.965 -0.610
ENSG00000170153 E004 11.2426409 0.0015591759 1.527198e-01 2.653659e-01 4 140868045 140868094 50 - 1.130 1.032 -0.354
ENSG00000170153 E005 26.0418580 0.0008095832 1.037019e-02 2.967317e-02 4 140868095 140868379 285 - 1.483 1.354 -0.446
ENSG00000170153 E006 0.1515154 0.0435419941 6.796913e-01   4 140911053 140911143 91 - 0.100 0.000 -9.152
ENSG00000170153 E007 22.8865177 0.0290055636 3.058805e-01 4.480227e-01 4 140911144 140911354 211 - 1.406 1.330 -0.264
ENSG00000170153 E008 13.2210832 0.0029372960 5.107869e-02 1.110921e-01 4 140925977 140926073 97 - 1.207 1.064 -0.512
ENSG00000170153 E009 0.5514428 0.0201086080 4.308494e-02 9.670987e-02 4 140944549 140944810 262 - 0.000 0.347 11.563
ENSG00000170153 E010 12.8913288 0.0015447081 2.123331e-03 7.606146e-03 4 140947654 140947736 83 - 1.234 0.980 -0.916
ENSG00000170153 E011 14.6527985 0.0106028911 2.116607e-01 3.404447e-01 4 140949301 140949372 72 - 1.227 1.147 -0.285
ENSG00000170153 E012 18.1666391 0.0080687901 9.609497e-02 1.845153e-01 4 140967623 140967748 126 - 1.323 1.217 -0.372
ENSG00000170153 E013 15.1015519 0.0158046162 2.423827e-01 3.769821e-01 4 140967749 140967873 125 - 1.240 1.158 -0.289
ENSG00000170153 E014 0.0000000       4 140996958 140997034 77 -      
ENSG00000170153 E015 2.2173913 0.0182853556 6.660773e-01 7.735990e-01 4 141053666 141053731 66 - 0.524 0.482 -0.203
ENSG00000170153 E016 0.4470576 0.0213333574 7.855226e-01 8.625700e-01 4 141053732 141053732 1 - 0.182 0.148 -0.356
ENSG00000170153 E017 31.3756391 0.0007253497 3.131971e-02 7.452087e-02 4 141132325 141133469 1145 - 1.545 1.458 -0.300
ENSG00000170153 E018 0.0000000       4 141212794 141212877 84 -