ENSG00000170035

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000392415 ENSG00000170035 HEK293_OSMI2_2hA HEK293_TMG_2hB UBE2E3 protein_coding protein_coding 82.58969 107.5077 79.42479 8.253173 0.3056364 -0.4367309 27.879231 43.803101 18.77079 5.3372209 0.9018769 -1.2221049 0.3295333 0.40463333 0.2363667 -0.16826667 4.284224e-06 3.094898e-06 FALSE TRUE
ENST00000410062 ENSG00000170035 HEK293_OSMI2_2hA HEK293_TMG_2hB UBE2E3 protein_coding protein_coding 82.58969 107.5077 79.42479 8.253173 0.3056364 -0.4367309 24.865010 34.194476 23.57039 0.8158753 1.5328065 -0.5365978 0.3001292 0.32110000 0.2969333 -0.02416667 7.937310e-01 3.094898e-06 FALSE TRUE
ENST00000602888 ENSG00000170035 HEK293_OSMI2_2hA HEK293_TMG_2hB UBE2E3 protein_coding retained_intron 82.58969 107.5077 79.42479 8.253173 0.3056364 -0.4367309 15.079643 19.077771 10.96732 2.6768505 1.6734348 -0.7981225 0.1842875 0.17756667 0.1381333 -0.03943333 5.698948e-01 3.094898e-06 FALSE FALSE
ENST00000602959 ENSG00000170035 HEK293_OSMI2_2hA HEK293_TMG_2hB UBE2E3 protein_coding protein_coding 82.58969 107.5077 79.42479 8.253173 0.3056364 -0.4367309 8.336361 4.326009 17.13357 1.1984998 4.0014750 1.9832283 0.1087833 0.04223333 0.2153667 0.17313333 4.564181e-03 3.094898e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000170035 E001 0.0000000       2 180967248 180967350 103 +      
ENSG00000170035 E002 0.3268771 0.0290565396 7.072775e-01   2 180980142 180980150 9 + 0.151 0.098 -0.713
ENSG00000170035 E003 0.8438645 0.0144878228 9.027468e-01 9.420759e-01 2 180980151 180980187 37 + 0.263 0.246 -0.128
ENSG00000170035 E004 0.6277178 0.0197122348 1.309641e-01 2.354856e-01 2 180980299 180980336 38 + 0.352 0.098 -2.299
ENSG00000170035 E005 2.0606922 0.0068316851 2.153986e-02 5.472624e-02 2 180980373 180980375 3 + 0.677 0.305 -1.884
ENSG00000170035 E006 3.3548365 0.0045721906 7.334565e-03 2.208799e-02 2 180980376 180980384 9 + 0.835 0.444 -1.714
ENSG00000170035 E007 3.5365409 0.0046582994 1.403978e-02 3.831617e-02 2 180980385 180980390 6 + 0.835 0.482 -1.521
ENSG00000170035 E008 3.5375851 0.0045596714 4.176886e-02 9.430361e-02 2 180980391 180980394 4 + 0.807 0.517 -1.244
ENSG00000170035 E009 4.6716943 0.0578925773 6.852227e-02 1.409192e-01 2 180980395 180980459 65 + 0.908 0.608 -1.212
ENSG00000170035 E010 4.4596992 0.0217627479 5.312620e-02 1.147120e-01 2 180980460 180980546 87 + 0.885 0.607 -1.132
ENSG00000170035 E011 35.2027908 0.0056688923 6.402482e-01 7.535278e-01 2 180980605 180980806 202 + 1.551 1.526 -0.089
ENSG00000170035 E012 109.7310805 0.0003832382 8.633619e-01 9.158584e-01 2 180980807 180980941 135 + 2.019 2.023 0.014
ENSG00000170035 E013 157.5233852 0.0012568956 1.946453e-02 5.033988e-02 2 180980942 180980973 32 + 2.221 2.152 -0.232
ENSG00000170035 E014 2.5174180 0.0059916068 8.721931e-07 7.336620e-06 2 180981036 180981108 73 + 0.885 0.098 -4.715
ENSG00000170035 E015 2.8098397 0.0053420793 1.338480e-07 1.327111e-06 2 180981109 180981123 15 + 0.930 0.098 -4.884
ENSG00000170035 E016 21.4740931 0.0260814131 2.433021e-04 1.147245e-03 2 180981124 180981317 194 + 1.546 1.169 -1.310
ENSG00000170035 E017 31.6828825 0.0208865519 8.815124e-03 2.582140e-02 2 180981318 180981385 68 + 1.634 1.411 -0.763
ENSG00000170035 E018 9.1211878 0.1051823578 1.585526e-01 2.731907e-01 2 180981386 180981477 92 + 1.116 0.916 -0.738
ENSG00000170035 E019 171.2648645 0.0005802271 3.273988e-04 1.490262e-03 2 180982018 180982022 5 + 2.275 2.180 -0.318
ENSG00000170035 E020 580.2769652 0.0001839305 7.317136e-06 5.014323e-05 2 180982023 180982236 214 + 2.782 2.724 -0.195
ENSG00000170035 E021 0.9328370 0.2616844162 6.541956e-01 7.644583e-01 2 180984006 180984042 37 + 0.353 0.242 -0.753
ENSG00000170035 E022 483.0446497 0.0011612807 3.358936e-01 4.797174e-01 2 180984043 180984093 51 + 2.672 2.659 -0.043
ENSG00000170035 E023 6.7147921 0.0024458394 9.750429e-02 1.866734e-01 2 180987338 180987394 57 + 0.989 0.802 -0.715
ENSG00000170035 E024 3.4790315 0.1826942970 7.956102e-02 1.588445e-01 2 180989882 180990046 165 + 0.859 0.446 -1.795
ENSG00000170035 E025 1.5383030 0.3072416495 7.735149e-01 8.538637e-01 2 181057691 181057692 2 + 0.352 0.405 0.303
ENSG00000170035 E026 757.4864029 0.0006358547 1.699452e-01 2.881426e-01 2 181057693 181057825 133 + 2.868 2.854 -0.046
ENSG00000170035 E027 923.3193299 0.0002501866 3.881958e-01 5.322998e-01 2 181060665 181060812 148 + 2.931 2.952 0.070
ENSG00000170035 E028 816.5856104 0.0008623340 3.445137e-01 4.886066e-01 2 181062791 181062889 99 + 2.874 2.899 0.083
ENSG00000170035 E029 851.5296265 0.0003893008 1.428496e-02 3.887499e-02 2 181062890 181063041 152 + 2.879 2.923 0.146
ENSG00000170035 E030 873.8202298 0.0017280487 1.069693e-06 8.822381e-06 2 181063042 181063441 400 + 2.842 2.953 0.369
ENSG00000170035 E031 2.3561301 0.0076510834 5.004757e-01 6.367261e-01 2 181075496 181075528 33 + 0.426 0.549 0.607
ENSG00000170035 E032 1.9111633 0.0080538528 7.851061e-01 8.622377e-01 2 181076196 181076198 3 + 0.426 0.481 0.284
ENSG00000170035 E033 6.4365630 0.0028825406 9.692973e-01 9.847933e-01 2 181076199 181076585 387 + 0.860 0.866 0.021