ENSG00000169925

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000303407 ENSG00000169925 HEK293_OSMI2_2hA HEK293_TMG_2hB BRD3 protein_coding protein_coding 18.77506 18.11216 18.86825 0.492868 0.3792437 0.0589699 14.472260 14.839766 14.035098 0.4086543 0.46758403 -0.0803735 0.7727333 0.8200000 0.74360000 -0.0764000 2.006469e-01 4.040321e-12 FALSE TRUE
ENST00000494743 ENSG00000169925 HEK293_OSMI2_2hA HEK293_TMG_2hB BRD3 protein_coding retained_intron 18.77506 18.11216 18.86825 0.492868 0.3792437 0.0589699 1.902855 2.075552 1.134101 0.2435956 0.02171193 -0.8662143 0.1016958 0.1146667 0.06016667 -0.0545000 1.248861e-02 4.040321e-12 FALSE FALSE
MSTRG.33581.4 ENSG00000169925 HEK293_OSMI2_2hA HEK293_TMG_2hB BRD3 protein_coding   18.77506 18.11216 18.86825 0.492868 0.3792437 0.0589699 1.062531 0.000000 2.463693 0.0000000 0.62847642 7.9505226 0.0544250 0.0000000 0.13046667 0.1304667 4.040321e-12 4.040321e-12 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000169925 E001 979.4002492 0.0096117580 1.261727e-08 1.526863e-07 9 134030305 134032013 1709 - 2.821 3.109 0.958
ENSG00000169925 E002 154.4026667 0.0036495289 3.836562e-06 2.802596e-05 9 134032014 134032165 152 - 2.045 2.302 0.861
ENSG00000169925 E003 238.8600188 0.0019620034 1.924162e-12 4.500884e-11 9 134032166 134032929 764 - 2.214 2.497 0.946
ENSG00000169925 E004 27.1203626 0.0104740364 3.066768e-01 4.488585e-01 9 134032930 134032944 15 - 1.370 1.518 0.511
ENSG00000169925 E005 55.2665452 0.0075074669 2.916456e-01 4.325245e-01 9 134032945 134032992 48 - 1.679 1.810 0.443
ENSG00000169925 E006 318.7619943 0.0002286265 5.546007e-05 3.089673e-04 9 134032993 134033401 409 - 2.512 2.499 -0.042
ENSG00000169925 E007 138.5962890 0.0003272351 1.981445e-09 2.776265e-08 9 134033402 134033488 87 - 2.197 2.082 -0.386
ENSG00000169925 E008 168.6592725 0.0002607982 1.115679e-02 3.154256e-02 9 134033489 134033705 217 - 2.230 2.228 -0.006
ENSG00000169925 E009 132.9645414 0.0034259772 3.241923e-02 7.666615e-02 9 134034701 134034826 126 - 2.136 2.115 -0.069
ENSG00000169925 E010 54.3998634 0.0007456089 2.824161e-04 1.308203e-03 9 134034827 134034829 3 - 1.792 1.683 -0.371
ENSG00000169925 E011 0.3268771 0.0285097156 9.960602e-01   9 134036028 134036031 4 - 0.115 0.130 0.203
ENSG00000169925 E012 217.6621432 0.0015273580 5.898700e-10 9.132067e-09 9 134036032 134036320 289 - 2.394 2.278 -0.387
ENSG00000169925 E013 55.6175244 0.0005357381 7.422304e-07 6.341360e-06 9 134036321 134036324 4 - 1.829 1.660 -0.572
ENSG00000169925 E014 5.1009783 0.0370285540 3.318892e-01 4.755292e-01 9 134036325 134036591 267 - 0.666 0.865 0.799
ENSG00000169925 E015 154.6166792 0.0015101303 1.858753e-05 1.161817e-04 9 134040034 134040269 236 - 2.229 2.153 -0.254
ENSG00000169925 E016 134.7951458 0.0048425384 6.334085e-03 1.949180e-02 9 134041760 134041951 192 - 2.159 2.103 -0.190
ENSG00000169925 E017 135.9508244 0.0107497198 1.320176e-04 6.676430e-04 9 134045293 134045421 129 - 2.208 2.056 -0.507
ENSG00000169925 E018 11.2952343 0.0015339047 1.095100e-06 9.013487e-06 9 134048002 134048082 81 - 0.694 1.282 2.203
ENSG00000169925 E019 228.9530967 0.0030661626 1.829886e-04 8.906697e-04 9 134048083 134048454 372 - 2.391 2.329 -0.206
ENSG00000169925 E020 2.1359180 0.0527302134 5.985524e-01 7.201225e-01 9 134049676 134049757 82 - 0.401 0.541 0.706
ENSG00000169925 E021 117.7477230 0.0053351477 8.662445e-02 1.699961e-01 9 134050374 134050549 176 - 2.080 2.065 -0.051
ENSG00000169925 E022 39.1264028 0.0037227084 2.714142e-01 4.101741e-01 9 134050550 134050588 39 - 1.600 1.597 -0.012
ENSG00000169925 E023 93.7029317 0.0041749397 2.825180e-01 4.225191e-01 9 134051562 134051709 148 - 1.965 1.980 0.049
ENSG00000169925 E024 73.7973614 0.0003598620 7.991423e-04 3.256579e-03 9 134052306 134052432 127 - 1.907 1.837 -0.236
ENSG00000169925 E025 35.5469477 0.0006293487 4.693634e-03 1.507826e-02 9 134052433 134052443 11 - 1.613 1.515 -0.334
ENSG00000169925 E026 76.6090350 0.0004466986 9.804344e-05 5.128258e-04 9 134053265 134053417 153 - 1.934 1.844 -0.305
ENSG00000169925 E027 59.2952962 0.0056345575 4.056065e-04 1.796329e-03 9 134053418 134053590 173 - 1.844 1.705 -0.469
ENSG00000169925 E028 2.5443066 0.0476551405 9.516924e-01 9.737331e-01 9 134053715 134054140 426 - 0.535 0.577 0.194
ENSG00000169925 E029 2.5140874 0.0134561464 3.423043e-02 8.019950e-02 9 134055776 134056012 237 - 0.281 0.686 2.079
ENSG00000169925 E030 23.9172843 0.0060357478 1.231765e-04 6.284618e-04 9 134067945 134068077 133 - 1.500 1.259 -0.837
ENSG00000169925 E031 1.0049449 0.1057037715 4.599770e-01 6.000348e-01 9 134068327 134068535 209 - 0.346 0.232 -0.788