ENSG00000169871

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000306085 ENSG00000169871 HEK293_OSMI2_2hA HEK293_TMG_2hB TRIM56 protein_coding protein_coding 0.9092358 1.259259 0.6121246 0.09632579 0.05653508 -1.028711 0.8566260 1.259259 0.52257020 0.09632579 0.03993158 -1.252943 0.93138333 1 0.8609667 -0.1390333 0.04861841 0.04861841 FALSE TRUE
ENST00000467847 ENSG00000169871 HEK293_OSMI2_2hA HEK293_TMG_2hB TRIM56 protein_coding protein_coding 0.9092358 1.259259 0.6121246 0.09632579 0.05653508 -1.028711 0.0526098 0.000000 0.08955439 0.00000000 0.04491890 3.315485 0.06861667 0 0.1390333 0.1390333 0.05830373 0.04861841   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000169871 E001 3.4863007 0.043097352 0.18775858 0.31090746 7 101085481 101085521 41 + 0.717 0.537 -0.789
ENSG00000169871 E002 3.7797665 0.016157560 0.12363078 0.22512785 7 101085522 101085571 50 + 0.759 0.559 -0.855
ENSG00000169871 E003 0.5858876 0.036224616 0.04114307 0.09314997 7 101086785 101087005 221 + 0.412 0.079 -2.979
ENSG00000169871 E004 6.7176432 0.023341287 0.18058809 0.30192096 7 101087006 101087168 163 + 0.923 0.783 -0.544
ENSG00000169871 E005 6.2917020 0.015054737 0.74945682 0.83641045 7 101087312 101087508 197 + 0.797 0.796 -0.007
ENSG00000169871 E006 11.1423579 0.021074242 0.03200550 0.07586865 7 101087509 101088128 620 + 1.168 0.958 -0.766
ENSG00000169871 E007 33.6277670 0.019760859 0.04706407 0.10393960 7 101088129 101089610 1482 + 1.548 1.438 -0.376
ENSG00000169871 E008 10.2824552 0.001729305 0.59568826 0.71774239 7 101089611 101089891 281 + 0.998 0.984 -0.052
ENSG00000169871 E009 12.1927607 0.001552406 0.53641915 0.66817673 7 101089892 101090170 279 + 1.062 1.043 -0.068
ENSG00000169871 E010 13.0730432 0.002347572 0.60457428 0.72505555 7 101090171 101090624 454 + 1.082 1.074 -0.027
ENSG00000169871 E011 30.4398976 0.021758804 0.66484540 0.77260278 7 101090625 101091637 1013 + 1.421 1.426 0.019
ENSG00000169871 E012 7.0652166 0.004967826 0.03871502 0.08865519 7 101091638 101091736 99 + 0.561 0.915 1.455
ENSG00000169871 E013 146.9360864 0.023726569 0.01169304 0.03280531 7 101091737 101097967 6231 + 1.959 2.138 0.598