ENSG00000169733

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000310496 ENSG00000169733 HEK293_OSMI2_2hA HEK293_TMG_2hB RFNG protein_coding protein_coding 36.39109 53.2201 26.18029 3.85621 0.1241328 -1.02321 14.145307 29.8044139 7.605069 2.0555866 0.8172082 -1.9690809 0.35264167 0.56033333 0.29026667 -0.270066667 1.811608e-07 1.811608e-07 FALSE TRUE
ENST00000429557 ENSG00000169733 HEK293_OSMI2_2hA HEK293_TMG_2hB RFNG protein_coding protein_coding 36.39109 53.2201 26.18029 3.85621 0.1241328 -1.02321 3.660572 3.0129030 5.238614 1.0001652 0.8754947 0.7960019 0.10722917 0.05693333 0.20040000 0.143466667 8.221927e-03 1.811608e-07 FALSE TRUE
ENST00000578676 ENSG00000169733 HEK293_OSMI2_2hA HEK293_TMG_2hB RFNG protein_coding retained_intron 36.39109 53.2201 26.18029 3.85621 0.1241328 -1.02321 2.586089 2.9842568 1.399324 0.2272456 0.4795643 -1.0871943 0.07166250 0.05610000 0.05336667 -0.002733333 8.952929e-01 1.811608e-07 TRUE FALSE
ENST00000580793 ENSG00000169733 HEK293_OSMI2_2hA HEK293_TMG_2hB RFNG protein_coding retained_intron 36.39109 53.2201 26.18029 3.85621 0.1241328 -1.02321 5.474786 7.0996924 3.296358 1.3500933 0.3196536 -1.1045441 0.15537917 0.13463333 0.12586667 -0.008766667 9.765429e-01 1.811608e-07 FALSE FALSE
ENST00000580953 ENSG00000169733 HEK293_OSMI2_2hA HEK293_TMG_2hB RFNG protein_coding retained_intron 36.39109 53.2201 26.18029 3.85621 0.1241328 -1.02321 2.671298 2.1879243 1.507037 0.3085719 0.8352624 -0.5348853 0.07720417 0.04076667 0.05786667 0.017100000 9.592320e-01 1.811608e-07 TRUE TRUE
ENST00000582478 ENSG00000169733 HEK293_OSMI2_2hA HEK293_TMG_2hB RFNG protein_coding retained_intron 36.39109 53.2201 26.18029 3.85621 0.1241328 -1.02321 2.308478 1.7817169 2.152884 0.4655402 0.4126619 0.2716134 0.07048333 0.03270000 0.08210000 0.049400000 2.054595e-02 1.811608e-07 TRUE TRUE
ENST00000583784 ENSG00000169733 HEK293_OSMI2_2hA HEK293_TMG_2hB RFNG protein_coding retained_intron 36.39109 53.2201 26.18029 3.85621 0.1241328 -1.02321 2.994419 2.7542441 2.501352 0.1944176 0.3184611 -0.1384206 0.09009583 0.05176667 0.09553333 0.043766667 1.266967e-02 1.811608e-07 FALSE TRUE
ENST00000584515 ENSG00000169733 HEK293_OSMI2_2hA HEK293_TMG_2hB RFNG protein_coding retained_intron 36.39109 53.2201 26.18029 3.85621 0.1241328 -1.02321 1.173518 0.2678934 1.545573 0.2678934 0.1572043 2.4848428 0.03755833 0.00440000 0.05900000 0.054600000 2.863145e-02 1.811608e-07 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000169733 E001 2.961058 0.0312134273 8.621845e-01 9.151321e-01 17 82047902 82047905 4 - 0.538 0.578 0.183
ENSG00000169733 E002 626.794112 0.0027160997 4.913124e-08 5.305384e-07 17 82047906 82048444 539 - 2.618 2.782 0.546
ENSG00000169733 E003 320.125996 0.0083464304 7.498481e-04 3.078347e-03 17 82048445 82048552 108 - 2.315 2.494 0.597
ENSG00000169733 E004 454.308298 0.0011975160 7.772325e-03 2.320709e-02 17 82048553 82048748 196 - 2.545 2.621 0.253
ENSG00000169733 E005 284.509828 0.0001809616 1.730224e-01 2.921199e-01 17 82048749 82048807 59 - 2.367 2.408 0.138
ENSG00000169733 E006 9.501350 0.0021166864 4.884378e-01 6.260060e-01 17 82048808 82049030 223 - 1.014 0.943 -0.262
ENSG00000169733 E007 266.453084 0.0002097460 1.694517e-01 2.874782e-01 17 82049031 82049116 86 - 2.336 2.379 0.143
ENSG00000169733 E008 58.891921 0.0087939407 3.474084e-05 2.032910e-04 17 82049117 82049676 560 - 1.898 1.637 -0.882
ENSG00000169733 E009 244.082334 0.0002887839 2.617238e-02 6.429887e-02 17 82049677 82049740 64 - 2.281 2.348 0.224
ENSG00000169733 E010 225.623682 0.0002837624 1.660016e-03 6.153901e-03 17 82049741 82049792 52 - 2.225 2.320 0.318
ENSG00000169733 E011 224.960990 0.0002113118 2.505680e-02 6.199148e-02 17 82049793 82049842 50 - 2.244 2.313 0.231
ENSG00000169733 E012 60.790297 0.0003947267 4.530948e-02 1.007575e-01 17 82049843 82049906 64 - 1.795 1.706 -0.299
ENSG00000169733 E013 49.570708 0.0004452363 5.086663e-03 1.616650e-02 17 82049907 82049917 11 - 1.744 1.605 -0.472
ENSG00000169733 E014 281.690280 0.0001713819 6.683676e-02 1.381175e-01 17 82049918 82050006 89 - 2.353 2.405 0.176
ENSG00000169733 E015 174.893345 0.0051662803 3.448426e-09 4.627383e-08 17 82050007 82050401 395 - 2.352 2.110 -0.809
ENSG00000169733 E016 172.471853 0.0003300258 5.805229e-03 1.810074e-02 17 82050402 82050414 13 - 2.233 2.160 -0.244
ENSG00000169733 E017 230.201581 0.0011401448 1.660230e-01 2.830249e-01 17 82050415 82050454 40 - 2.328 2.297 -0.103
ENSG00000169733 E018 305.607979 0.0005503755 2.744948e-02 6.690366e-02 17 82050455 82050555 101 - 2.458 2.416 -0.142
ENSG00000169733 E019 49.929000 0.0004798485 5.956577e-11 1.090085e-09 17 82050556 82050661 106 - 1.858 1.535 -1.098
ENSG00000169733 E020 258.351292 0.0003886867 2.779447e-05 1.665848e-04 17 82050662 82050764 103 - 2.421 2.327 -0.313
ENSG00000169733 E021 40.814924 0.0241577461 8.491098e-08 8.740562e-07 17 82050765 82050926 162 - 1.858 1.377 -1.640
ENSG00000169733 E022 31.877198 0.0117442252 1.228007e-06 1.000569e-05 17 82050927 82051011 85 - 1.700 1.323 -1.293
ENSG00000169733 E023 45.999414 0.0072372973 2.377421e-07 2.245689e-06 17 82051012 82051293 282 - 1.828 1.498 -1.120
ENSG00000169733 E024 137.002979 0.0004014821 2.761485e-01 4.155586e-01 17 82051294 82051342 49 - 2.101 2.073 -0.093
ENSG00000169733 E025 7.270032 0.0248944567 2.982372e-01 4.396694e-01 17 82051343 82051434 92 - 0.970 0.834 -0.520
ENSG00000169733 E026 90.554251 0.0007985827 3.312677e-01 4.748685e-01 17 82051500 82051831 332 - 1.924 1.892 -0.107