ENSG00000169519

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000303459 ENSG00000169519 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL15 protein_coding protein_coding 6.163524 1.772234 9.768814 0.362837 0.2626701 2.455973 0.9599542 0.60440580 2.0847679 0.08979123 0.13640409 1.7695267 0.20955417 0.34883333 0.21313333 -0.13570000 4.164313e-02 2.434378e-05 FALSE TRUE
ENST00000403099 ENSG00000169519 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL15 protein_coding protein_coding 6.163524 1.772234 9.768814 0.362837 0.2626701 2.455973 0.3330658 0.00000000 0.6842238 0.00000000 0.25095674 6.1173289 0.04739167 0.00000000 0.06913333 0.06913333 1.263049e-02 2.434378e-05   FALSE
ENST00000407364 ENSG00000169519 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL15 protein_coding protein_coding 6.163524 1.772234 9.768814 0.362837 0.2626701 2.455973 2.3147725 0.12256483 2.8326595 0.12256483 0.41013274 4.4224713 0.30285833 0.09726667 0.28953333 0.19226667 1.978122e-01 2.434378e-05 FALSE TRUE
ENST00000437814 ENSG00000169519 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL15 protein_coding nonsense_mediated_decay 6.163524 1.772234 9.768814 0.362837 0.2626701 2.455973 0.4016400 0.50334155 0.8653163 0.12108225 0.08932236 0.7698854 0.10730417 0.27996667 0.08873333 -0.19123333 2.434378e-05 2.434378e-05 TRUE FALSE
MSTRG.5321.11 ENSG00000169519 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL15 protein_coding   6.163524 1.772234 9.768814 0.362837 0.2626701 2.455973 0.1702508 0.00000000 0.5229630 0.00000000 0.52296296 5.7359634 0.01728750 0.00000000 0.05656667 0.05656667 1.000000e+00 2.434378e-05 FALSE TRUE
MSTRG.5321.7 ENSG00000169519 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL15 protein_coding   6.163524 1.772234 9.768814 0.362837 0.2626701 2.455973 0.2004662 0.35427514 0.5224047 0.17795454 0.27579435 0.5474946 0.06157917 0.18576667 0.05206667 -0.13370000 7.023444e-01 2.434378e-05 TRUE TRUE
MSTRG.5321.9 ENSG00000169519 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL15 protein_coding   6.163524 1.772234 9.768814 0.362837 0.2626701 2.455973 0.7295624 0.01211301 0.2985669 0.01211301 0.29856686 3.8026157 0.06968750 0.00490000 0.02976667 0.02486667 8.628645e-01 2.434378e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000169519 E001 0.1515154 0.0448076097 1.000000e+00   11 28108248 28108319 72 + 0.055 0.000 -8.917
ENSG00000169519 E002 0.1515154 0.0448076097 1.000000e+00   11 28108320 28108329 10 + 0.055 0.000 -10.781
ENSG00000169519 E003 0.3030308 0.4640671096 1.000000e+00   11 28108330 28108386 57 + 0.105 0.000 -11.152
ENSG00000169519 E004 0.1515154 0.0448076097 1.000000e+00   11 28108387 28108387 1 + 0.055 0.000 -10.781
ENSG00000169519 E005 0.3030308 0.4640671096 1.000000e+00   11 28108388 28108392 5 + 0.105 0.000 -11.152
ENSG00000169519 E006 0.7416694 0.0172825068 7.720239e-01 8.528687e-01 11 28108393 28108397 5 + 0.189 0.250 0.518
ENSG00000169519 E007 1.4759519 0.0114728147 6.477644e-01 7.593969e-01 11 28108398 28108403 6 + 0.347 0.250 -0.651
ENSG00000169519 E008 4.6449329 0.0510918129 6.046361e-01 7.250972e-01 11 28108404 28108452 49 + 0.643 0.750 0.447
ENSG00000169519 E009 0.7447899 0.0333587258 1.911025e-01 3.151252e-01 11 28108453 28108454 2 + 0.149 0.407 1.928
ENSG00000169519 E010 10.1671320 0.0097432389 9.175908e-01 9.519236e-01 11 28110166 28110311 146 + 0.948 0.943 -0.020
ENSG00000169519 E011 8.4558890 0.0136418281 5.937611e-01 7.160759e-01 11 28110312 28110314 3 + 0.860 0.943 0.316
ENSG00000169519 E012 19.7292331 0.0093482761 9.490723e-01 9.720803e-01 11 28110315 28110401 87 + 1.206 1.218 0.044
ENSG00000169519 E013 13.3663917 0.0078950257 6.794515e-01 7.836271e-01 11 28113318 28113342 25 + 1.059 1.014 -0.164
ENSG00000169519 E014 26.5004870 0.0098803673 6.372921e-01 7.512197e-01 11 28113343 28113429 87 + 1.335 1.293 -0.146
ENSG00000169519 E015 42.4403625 0.0024537697 2.071757e-01 3.351391e-01 11 28113430 28113604 175 + 1.507 1.601 0.323
ENSG00000169519 E016 3.1104875 0.0075590225 8.450080e-03 2.491079e-02 11 28122142 28122180 39 + 0.420 0.859 1.932
ENSG00000169519 E017 6.3011126 0.0037931182 2.377374e-02 5.935118e-02 11 28123862 28123922 61 + 0.694 1.015 1.245
ENSG00000169519 E018 8.7640374 0.0022229573 1.998082e-03 7.219735e-03 11 28125510 28125866 357 + 0.799 1.177 1.403
ENSG00000169519 E019 2.5107580 0.1824891235 3.973952e-01 5.411499e-01 11 28134546 28134645 100 + 0.420 0.676 1.199
ENSG00000169519 E020 1.9260163 0.0275848253 4.914186e-01 6.286846e-01 11 28145504 28145747 244 + 0.373 0.522 0.775
ENSG00000169519 E021 0.0000000       11 28163329 28164136 808 +      
ENSG00000169519 E022 0.0000000       11 28197441 28197549 109 +      
ENSG00000169519 E023 16.1887897 0.0011517268 2.345010e-01 3.677609e-01 11 28211062 28211198 137 + 1.143 1.015 -0.465
ENSG00000169519 E024 24.2356589 0.0009006728 2.099028e-03 7.531193e-03 11 28290206 28290397 192 + 1.333 1.015 -1.134
ENSG00000169519 E025 15.7994478 0.0011237624 2.032380e-03 7.327172e-03 11 28296753 28296796 44 + 1.164 0.754 -1.539
ENSG00000169519 E026 32.2806618 0.0006684549 1.592509e-04 7.884798e-04 11 28296797 28296931 135 + 1.452 1.103 -1.225
ENSG00000169519 E027 0.0000000       11 28327581 28328094 514 +      
ENSG00000169519 E028 5.5984717 0.0029656695 7.787429e-01 8.576461e-01 11 28328095 28328165 71 + 0.728 0.690 -0.159
ENSG00000169519 E029 58.2262286 0.0055823453 1.029490e-04 5.356452e-04 11 28330396 28330760 365 + 1.692 1.401 -0.996
ENSG00000169519 E030 83.2234830 0.0035535080 4.377359e-01 5.796347e-01 11 28330761 28332182 1422 + 1.813 1.780 -0.113
ENSG00000169519 E031 103.8612527 0.0044421000 1.891708e-06 1.479924e-05 11 28332183 28333507 1325 + 1.856 2.076 0.737
ENSG00000169519 E032 0.5911862 0.0193874923 6.666628e-01 7.740772e-01 11 28352090 28352158 69 + 0.189 0.000 -12.653
ENSG00000169519 E033 0.5975289 0.0620379745 6.689155e-01 7.758331e-01 11 28361937 28362036 100 + 0.189 0.000 -12.575
ENSG00000169519 E034 0.1515154 0.0448076097 1.000000e+00   11 28398788 28399086 299 + 0.055 0.000 -10.781
ENSG00000169519 E035 0.0000000       11 28410676 28410799 124 +      
ENSG00000169519 E036 0.1515154 0.0448076097 1.000000e+00   11 28424299 28424364 66 + 0.055 0.000 -10.781
ENSG00000169519 E037 0.1482932 0.0411597534 6.631967e-02   11 28526478 28526520 43 + 0.000 0.250 13.536
ENSG00000169519 E038 0.7760110 0.0168380958 1.819415e-01 3.036423e-01 11 28526616 28527041 426 + 0.149 0.408 1.933