ENSG00000169515

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000307522 ENSG00000169515 HEK293_OSMI2_2hA HEK293_TMG_2hB CCDC8 protein_coding protein_coding 16.24698 25.86648 10.05669 0.4339406 0.1097348 -1.362053 14.0871472 22.94871554 8.5161340 0.55483679 0.2691684 -1.429078 0.87560833 0.887633333 0.84750000 -0.04013333 7.265411e-01 2.028753e-07 FALSE  
MSTRG.17360.2 ENSG00000169515 HEK293_OSMI2_2hA HEK293_TMG_2hB CCDC8 protein_coding   16.24698 25.86648 10.05669 0.4339406 0.1097348 -1.362053 0.3833736 1.68172736 0.0000000 0.47216903 0.0000000 -7.402353 0.01737500 0.065033333 0.00000000 -0.06503333 2.028753e-07 2.028753e-07 FALSE  
MSTRG.17360.6 ENSG00000169515 HEK293_OSMI2_2hA HEK293_TMG_2hB CCDC8 protein_coding   16.24698 25.86648 10.05669 0.4339406 0.1097348 -1.362053 0.4841446 0.07701607 0.6032338 0.07701607 0.6032338 2.817083 0.03009583 0.003066667 0.05996667 0.05690000 9.269212e-01 2.028753e-07 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000169515 E001 185.58636 0.0013584664 1.882302e-02 4.894329e-02 19 46410329 46410796 468 - 2.123 2.191 0.226
ENSG00000169515 E002 248.26945 0.0012094351 1.881897e-06 1.472918e-05 19 46410797 46411168 372 - 2.191 2.327 0.454
ENSG00000169515 E003 324.79374 0.0001778211 7.027435e-04 2.908986e-03 19 46411169 46411409 241 - 2.367 2.430 0.211
ENSG00000169515 E004 286.24028 0.0019135816 3.715293e-02 8.576751e-02 19 46411410 46411628 219 - 2.323 2.372 0.164
ENSG00000169515 E005 88.49313 0.0083412441 9.707726e-01 9.857530e-01 19 46411629 46411645 17 - 1.871 1.858 -0.044
ENSG00000169515 E006 107.99083 0.0003686802 7.515274e-01 8.379512e-01 19 46411646 46411700 55 - 1.947 1.944 -0.011
ENSG00000169515 E007 91.24478 0.0057750923 9.833884e-01 9.936856e-01 19 46411701 46411717 17 - 1.881 1.863 -0.064
ENSG00000169515 E008 74.77980 0.0009029259 3.805255e-01 5.248058e-01 19 46411718 46411724 7 - 1.831 1.772 -0.199
ENSG00000169515 E009 117.15305 0.0002858625 9.178709e-01 9.520956e-01 19 46411725 46411789 65 - 1.991 1.971 -0.069
ENSG00000169515 E010 64.73503 0.0049304209 2.075822e-01 3.355818e-01 19 46411790 46411796 7 - 1.792 1.699 -0.315
ENSG00000169515 E011 87.27679 0.0022653209 9.314659e-01 9.608314e-01 19 46411797 46411820 24 - 1.857 1.844 -0.044
ENSG00000169515 E012 96.06205 0.0014739202 2.649088e-01 4.029141e-01 19 46411821 46411861 41 - 1.941 1.874 -0.227
ENSG00000169515 E013 134.85720 0.0004153606 2.464332e-01 3.817712e-01 19 46411862 46411916 55 - 2.083 2.025 -0.194
ENSG00000169515 E014 810.75354 0.0022371869 2.064594e-05 1.276545e-04 19 46411917 46413564 1648 - 2.892 2.790 -0.341