ENSG00000169446

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000305963 ENSG00000169446 HEK293_OSMI2_2hA HEK293_TMG_2hB MMGT1 protein_coding protein_coding 25.582 16.76471 36.80531 3.005613 0.8938661 1.134018 18.886904 16.5383477 23.78503 3.0831787 1.4638432 0.5239728 0.7965667 0.9846 0.6456333 -0.3389667 0.0007805434 0.0007805434 FALSE FALSE
ENST00000681201 ENSG00000169446 HEK293_OSMI2_2hA HEK293_TMG_2hB MMGT1 protein_coding protein_coding 25.582 16.76471 36.80531 3.005613 0.8938661 1.134018 6.146861 0.2124986 11.36455 0.2124986 0.2214447 5.6758691 0.1874458 0.0148 0.3088667 0.2940667 0.0085637777 0.0007805434   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000169446 E001 1418.13913 0.0121088835 1.242391e-11 2.547815e-10 X 135960588 135963172 2585 - 2.948 3.349 1.332
ENSG00000169446 E002 1261.68577 0.0023473819 4.533689e-05 2.581354e-04 X 135963173 135964677 1505 - 3.050 3.133 0.277
ENSG00000169446 E003 76.23716 0.0079216153 8.586570e-01 9.128102e-01 X 135964678 135964732 55 - 1.812 1.958 0.491
ENSG00000169446 E004 302.27636 0.0064779374 4.427748e-05 2.527034e-04 X 135964733 135965183 451 - 2.467 2.449 -0.061
ENSG00000169446 E005 170.08829 0.0005457403 2.594554e-28 3.772298e-26 X 135967390 135967493 104 - 2.264 2.094 -0.568
ENSG00000169446 E006 117.72259 0.0105582502 9.055397e-09 1.125299e-07 X 135971058 135971110 53 - 2.112 1.910 -0.678
ENSG00000169446 E007 169.53124 0.0168509479 2.029448e-07 1.942469e-06 X 135973597 135973822 226 - 2.272 2.059 -0.713
ENSG00000169446 E008 72.01347 0.0069012042 2.900619e-10 4.732291e-09 X 135973823 135973899 77 - 1.908 1.677 -0.781
ENSG00000169446 E009 69.45513 0.0077303111 4.555068e-10 7.186409e-09 X 135973900 135974029 130 - 1.895 1.653 -0.818