ENSG00000169330

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000305428 ENSG00000169330 HEK293_OSMI2_2hA HEK293_TMG_2hB MINAR1 protein_coding protein_coding 0.9940633 0.7667853 1.063841 0.1266812 0.0568963 0.4671923 0.5088823 0.1346084 0.7844686 0.10435472 0.2383904 2.4578391 0.4927708 0.1468333 0.7176333 0.5708 1.891553e-01 7.78576e-12 FALSE TRUE
ENST00000559272 ENSG00000169330 HEK293_OSMI2_2hA HEK293_TMG_2hB MINAR1 protein_coding nonsense_mediated_decay 0.9940633 0.7667853 1.063841 0.1266812 0.0568963 0.4671923 0.3178302 0.1752995 0.2793722 0.09845904 0.1823575 0.6430669 0.3038917 0.2690667 0.2823667 0.0133 9.988268e-01 7.78576e-12 TRUE TRUE
MSTRG.11338.3 ENSG00000169330 HEK293_OSMI2_2hA HEK293_TMG_2hB MINAR1 protein_coding   0.9940633 0.7667853 1.063841 0.1266812 0.0568963 0.4671923 0.1673507 0.4568774 0.0000000 0.10962838 0.0000000 -5.5449717 0.2033292 0.5841000 0.0000000 -0.5841 7.785760e-12 7.78576e-12   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000169330 E001 0.4502799 0.0253186456 2.295024e-01 3.618358e-01 15 79432336 79432540 205 + 0.235 0.000 -10.072
ENSG00000169330 E002 0.0000000       15 79456096 79456097 2 +      
ENSG00000169330 E003 38.8326378 0.0007391768 6.268342e-08 6.623082e-07 15 79456098 79458445 2348 + 1.684 1.389 -1.007
ENSG00000169330 E004 8.1303196 0.0025380001 2.252958e-02 5.678132e-02 15 79463067 79463321 255 + 1.042 0.769 -1.039
ENSG00000169330 E005 0.7813216 0.0783769188 2.807154e-01 4.205869e-01 15 79463695 79463760 66 + 0.170 0.366 1.466
ENSG00000169330 E006 0.8189741 0.0164630153 4.701001e-01 6.093141e-01 15 79464466 79464500 35 + 0.291 0.160 -1.104
ENSG00000169330 E007 21.3509855 0.0009768122 5.418502e-02 1.165744e-01 15 79468187 79468950 764 + 1.383 1.241 -0.495
ENSG00000169330 E008 81.3379283 0.0005803295 1.146283e-12 2.784100e-11 15 79468951 79472304 3354 + 1.813 2.004 0.644