ENSG00000169299

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000381967 ENSG00000169299 HEK293_OSMI2_2hA HEK293_TMG_2hB PGM2 protein_coding protein_coding 12.28101 4.633587 18.94739 0.5160862 0.739663 2.029449 9.9667167 4.3031571 14.655587 0.5314926 0.08410902 1.765619 0.84465833 0.92670000 0.77560000 -0.15110000 1.507261e-03 1.295799e-10 FALSE TRUE
ENST00000505986 ENSG00000169299 HEK293_OSMI2_2hA HEK293_TMG_2hB PGM2 protein_coding nonsense_mediated_decay 12.28101 4.633587 18.94739 0.5160862 0.739663 2.029449 0.6869687 0.0000000 1.558022 0.0000000 0.00806206 7.292802 0.03685000 0.00000000 0.08246667 0.08246667 1.295799e-10 1.295799e-10 FALSE TRUE
ENST00000625312 ENSG00000169299 HEK293_OSMI2_2hA HEK293_TMG_2hB PGM2 protein_coding protein_coding 12.28101 4.633587 18.94739 0.5160862 0.739663 2.029449 1.1152235 0.2171228 2.200320 0.1326249 0.70785775 3.282711 0.07689167 0.04686667 0.11353333 0.06666667 4.442142e-01 1.295799e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000169299 E001 2.065990 0.0073440095 2.404387e-01 3.746599e-01 4 37826660 37826685 26 + 0.389 0.594 1.016
ENSG00000169299 E002 6.468624 0.0026056039 7.373925e-01 8.275877e-01 4 37826686 37826707 22 + 0.843 0.797 -0.180
ENSG00000169299 E003 11.404919 0.0015591158 7.378898e-01 8.279349e-01 4 37826708 37826718 11 + 1.053 1.016 -0.138
ENSG00000169299 E004 17.895214 0.0010738631 6.020565e-01 7.230807e-01 4 37826719 37826720 2 + 1.242 1.194 -0.170
ENSG00000169299 E005 54.429178 0.0004485793 5.851989e-04 2.478907e-03 4 37826721 37826813 93 + 1.742 1.543 -0.677
ENSG00000169299 E006 111.276808 0.0003703376 1.084877e-07 1.094800e-06 4 37829964 37830131 168 + 2.050 1.831 -0.737
ENSG00000169299 E007 87.418933 0.0008326348 2.638452e-03 9.185322e-03 4 37834618 37834724 107 + 1.934 1.795 -0.466
ENSG00000169299 E008 69.011488 0.0003527416 3.666186e-01 5.109628e-01 4 37837529 37837613 85 + 1.810 1.766 -0.147
ENSG00000169299 E009 2.016774 0.0089012698 4.744115e-01 6.132423e-01 4 37839517 37839683 167 + 0.476 0.337 -0.762
ENSG00000169299 E010 44.418386 0.0014810450 5.356739e-01 6.674766e-01 4 37839848 37839857 10 + 1.622 1.585 -0.128
ENSG00000169299 E011 68.161446 0.0003455368 2.069553e-01 3.348956e-01 4 37839858 37839931 74 + 1.810 1.748 -0.207
ENSG00000169299 E012 121.441195 0.0003723648 5.044023e-02 1.099512e-01 4 37840066 37840259 194 + 2.059 1.987 -0.244
ENSG00000169299 E013 133.547525 0.0003937643 5.252376e-01 6.586399e-01 4 37844364 37844553 190 + 2.086 2.063 -0.075
ENSG00000169299 E014 98.966766 0.0004575440 7.671067e-01 8.494219e-01 4 37845633 37845730 98 + 1.957 1.945 -0.039
ENSG00000169299 E015 113.564723 0.0002760227 8.861630e-01 9.312030e-01 4 37846931 37847039 109 + 2.014 2.010 -0.017
ENSG00000169299 E016 80.468530 0.0026181964 7.207454e-01 8.153041e-01 4 37847040 37847111 72 + 1.871 1.854 -0.055
ENSG00000169299 E017 4.433995 0.0052893708 7.142626e-01 8.105207e-01 4 37847112 37847201 90 + 0.713 0.654 -0.245
ENSG00000169299 E018 89.297981 0.0056884083 4.317302e-01 5.742630e-01 4 37847202 37847295 94 + 1.920 1.882 -0.126
ENSG00000169299 E019 4.517745 0.0037153574 5.701760e-02 1.214902e-01 4 37847296 37847478 183 + 0.770 0.440 -1.476
ENSG00000169299 E020 93.756025 0.0008630830 1.226300e-01 2.237061e-01 4 37848522 37848651 130 + 1.912 1.976 0.213
ENSG00000169299 E021 117.021521 0.0003309970 1.769979e-02 4.648662e-02 4 37850184 37850373 190 + 1.999 2.083 0.281
ENSG00000169299 E022 103.206307 0.0003302381 3.861653e-02 8.847779e-02 4 37855608 37855741 134 + 1.948 2.027 0.264
ENSG00000169299 E023 228.216467 0.0002086644 3.009713e-15 1.070964e-13 4 37861510 37862937 1428 + 2.255 2.443 0.627