ENSG00000169251

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000351193 ENSG00000169251 HEK293_OSMI2_2hA HEK293_TMG_2hB NMD3 protein_coding protein_coding 26.65973 5.231547 45.19762 0.4143884 0.903547 3.108501 15.461638 3.627326 24.503369 1.0426792 1.726640 2.752619 0.62047917 0.6734667 0.54100000 -0.13246667 6.757260e-01 6.365217e-10 FALSE TRUE
ENST00000473909 ENSG00000169251 HEK293_OSMI2_2hA HEK293_TMG_2hB NMD3 protein_coding retained_intron 26.65973 5.231547 45.19762 0.4143884 0.903547 3.108501 5.205856 0.000000 9.561938 0.0000000 2.126507 9.902667 0.10530833 0.0000000 0.21360000 0.21360000 6.365217e-10 6.365217e-10 FALSE TRUE
ENST00000493066 ENSG00000169251 HEK293_OSMI2_2hA HEK293_TMG_2hB NMD3 protein_coding protein_coding 26.65973 5.231547 45.19762 0.4143884 0.903547 3.108501 1.660929 0.000000 2.980795 0.0000000 1.499030 8.224385 0.03488333 0.0000000 0.06476667 0.06476667 4.239773e-01 6.365217e-10 FALSE FALSE
MSTRG.24022.3 ENSG00000169251 HEK293_OSMI2_2hA HEK293_TMG_2hB NMD3 protein_coding   26.65973 5.231547 45.19762 0.4143884 0.903547 3.108501 1.999701 1.211495 3.884851 0.7335953 0.534243 1.672920 0.13726667 0.2490333 0.08636667 -0.16266667 8.899703e-01 6.365217e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000169251 E001 0.0000000       3 161104696 161104821 126 +      
ENSG00000169251 E002 0.1472490 0.0444435502 7.707790e-01   3 161104822 161104881 60 + 0.045 0.000 -7.599
ENSG00000169251 E003 0.0000000       3 161104882 161104885 4 +      
ENSG00000169251 E004 0.0000000       3 161156971 161157055 85 +      
ENSG00000169251 E005 0.1451727 0.0432091819 7.688736e-01   3 161221254 161221261 8 + 0.045 0.000 -7.583
ENSG00000169251 E006 0.1451727 0.0432091819 7.688736e-01   3 161221262 161221275 14 + 0.045 0.000 -7.583
ENSG00000169251 E007 2.1005493 0.0067034383 5.377672e-01 6.692839e-01 3 161221276 161221287 12 + 0.385 0.293 -0.568
ENSG00000169251 E008 5.8469879 0.0267460303 3.928882e-01 5.367788e-01 3 161221288 161221307 20 + 0.695 0.590 -0.450
ENSG00000169251 E009 10.7577437 0.0016092564 1.731914e-02 4.564571e-02 3 161221308 161221310 3 + 0.934 0.590 -1.392
ENSG00000169251 E010 26.8580189 0.0066984975 7.134449e-04 2.946717e-03 3 161221311 161221323 13 + 1.297 0.941 -1.283
ENSG00000169251 E011 41.3893706 0.0015773395 5.849648e-05 3.239588e-04 3 161221324 161221331 8 + 1.476 1.161 -1.099
ENSG00000169251 E012 53.7540637 0.0004584413 1.103093e-03 4.313263e-03 3 161221332 161221409 78 + 1.574 1.400 -0.599
ENSG00000169251 E013 10.5517935 0.0951145575 7.018594e-01 8.008383e-01 3 161221410 161221556 147 + 0.867 1.037 0.634
ENSG00000169251 E014 3.0811423 0.1568778592 8.569223e-01 9.115794e-01 3 161221557 161221558 2 + 0.473 0.472 -0.001
ENSG00000169251 E015 13.9767845 0.0054302826 7.175296e-02 1.462661e-01 3 161221559 161221724 166 + 1.024 0.832 -0.725
ENSG00000169251 E016 0.1451727 0.0432091819 7.688736e-01   3 161221725 161221735 11 + 0.045 0.000 -7.583
ENSG00000169251 E017 0.4418608 0.0249176316 1.000000e+00 1.000000e+00 3 161221736 161221993 258 + 0.124 0.000 -9.169
ENSG00000169251 E018 103.6488456 0.0029342622 1.498848e-05 9.568093e-05 3 161221994 161222057 64 + 1.856 1.665 -0.644
ENSG00000169251 E019 128.3686537 0.0021668467 1.624415e-06 1.288588e-05 3 161224930 161225018 89 + 1.947 1.762 -0.625
ENSG00000169251 E020 109.8230368 0.0020549257 3.660529e-08 4.051863e-07 3 161225019 161225064 46 + 1.887 1.627 -0.878
ENSG00000169251 E021 145.4877856 0.0002094489 7.095396e-09 8.991339e-08 3 161227247 161227343 97 + 2.002 1.804 -0.668
ENSG00000169251 E022 123.6657796 0.0003025088 2.562741e-03 8.950682e-03 3 161233399 161233479 81 + 1.921 1.859 -0.208
ENSG00000169251 E023 144.8093912 0.0008459517 2.623780e-01 4.001669e-01 3 161234727 161234855 129 + 1.976 2.013 0.125
ENSG00000169251 E024 0.2987644 0.0274652995 1.000000e+00   3 161234856 161234905 50 + 0.086 0.000 -8.584
ENSG00000169251 E025 110.9059584 0.0002644612 4.643687e-01 6.041003e-01 3 161235122 161235212 91 + 1.849 1.971 0.411
ENSG00000169251 E026 0.7728905 0.0145546432 6.096202e-01 7.290896e-01 3 161235213 161235331 119 + 0.158 0.293 1.132
ENSG00000169251 E027 127.5539463 0.0007178020 3.821263e-01 5.263624e-01 3 161238113 161238191 79 + 1.920 1.966 0.155
ENSG00000169251 E028 154.0655611 0.0002102356 1.195132e-02 3.341513e-02 3 161238730 161238826 97 + 2.009 1.988 -0.070
ENSG00000169251 E029 162.8406139 0.0002689365 1.516181e-03 5.688631e-03 3 161241046 161241163 118 + 2.036 1.988 -0.161
ENSG00000169251 E030 153.2268383 0.0002484782 2.579724e-01 3.951751e-01 3 161242508 161242653 146 + 2.000 2.041 0.136
ENSG00000169251 E031 126.9898785 0.0002367602 4.694641e-01 6.087075e-01 3 161246336 161246448 113 + 1.916 1.971 0.184
ENSG00000169251 E032 119.5330445 0.0002371415 3.840377e-01 5.282383e-01 3 161247258 161247330 73 + 1.892 1.938 0.156
ENSG00000169251 E033 166.7018264 0.0002620207 3.991103e-01 5.428456e-01 3 161249454 161249560 107 + 2.034 2.088 0.182
ENSG00000169251 E034 141.7905919 0.0062943405 6.534266e-01 7.639029e-01 3 161250256 161250326 71 + 1.963 2.019 0.188
ENSG00000169251 E035 191.3857615 0.0008370079 6.023527e-01 7.233296e-01 3 161250780 161250890 111 + 2.091 2.157 0.223
ENSG00000169251 E036 570.2638845 0.0111120826 1.813923e-13 4.975996e-12 3 161250891 161252307 1417 + 2.498 2.904 1.352
ENSG00000169251 E037 6.6996742 0.0024745732 5.721670e-02 1.218377e-01 3 161252769 161253532 764 + 0.676 1.026 1.365