ENSG00000169032

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000307102 ENSG00000169032 HEK293_OSMI2_2hA HEK293_TMG_2hB MAP2K1 protein_coding protein_coding 30.64242 32.22536 31.41168 3.575559 0.6903155 -0.03688348 26.0740373 25.724890 28.1445459 1.587246 0.49637171 0.1296422 0.8531250 0.80666667 0.896266667 0.0896 0.147091760 0.001009917 FALSE  
ENST00000687481 ENSG00000169032 HEK293_OSMI2_2hA HEK293_TMG_2hB MAP2K1 protein_coding retained_intron 30.64242 32.22536 31.41168 3.575559 0.6903155 -0.03688348 0.9491727 3.299376 0.1203661 1.633687 0.08329664 -4.6659187 0.0286625 0.09446667 0.003766667 -0.0907 0.001009917 0.001009917 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000169032 E001 5.0239546 0.0160700407 7.590628e-01 8.435184e-01 15 66386837 66386911 75 + 0.799 0.785 -0.056
ENSG00000169032 E002 292.5586982 0.0021028682 1.319876e-03 5.043396e-03 15 66386912 66387427 516 + 2.504 2.435 -0.228
ENSG00000169032 E003 0.2214452 0.0422703664 5.245084e-01   15 66401741 66401878 138 + 0.000 0.121 10.802
ENSG00000169032 E004 0.0000000       15 66401879 66401879 1 +      
ENSG00000169032 E005 0.0000000       15 66401880 66401947 68 +      
ENSG00000169032 E006 372.6063678 0.0008430429 3.696757e-09 4.930232e-08 15 66435027 66435237 211 + 2.624 2.524 -0.334
ENSG00000169032 E007 324.3816147 0.0002461107 5.609393e-06 3.947705e-05 15 66436746 66436892 147 + 2.545 2.484 -0.202
ENSG00000169032 E008 267.7370785 0.0001726814 2.699504e-03 9.372544e-03 15 66443280 66443357 78 + 2.449 2.415 -0.113
ENSG00000169032 E009 248.3019466 0.0002027771 4.650908e-03 1.496053e-02 15 66444656 66444707 52 + 2.416 2.382 -0.111
ENSG00000169032 E010 0.3634088 0.4708111071 3.353838e-01   15 66444708 66444966 259 + 0.000 0.216 11.810
ENSG00000169032 E011 0.2966881 0.0290785164 1.785773e-01   15 66453454 66453543 90 + 0.221 0.000 -12.168
ENSG00000169032 E012 0.0000000       15 66458706 66458756 51 +      
ENSG00000169032 E013 299.8107477 0.0001691704 1.656573e-02 4.398497e-02 15 66481755 66481879 125 + 2.490 2.472 -0.060
ENSG00000169032 E014 2.6445114 0.0240237625 1.106863e-01 2.065402e-01 15 66484882 66484989 108 + 0.366 0.656 1.417
ENSG00000169032 E015 99.8609983 0.0003556455 2.721506e-01 4.110069e-01 15 66484990 66484993 4 + 2.014 2.006 -0.026
ENSG00000169032 E016 298.4960087 0.0007453840 5.927475e-01 7.152370e-01 15 66484994 66485191 198 + 2.455 2.497 0.138
ENSG00000169032 E017 2.1119845 0.0069510517 9.328166e-01 9.616696e-01 15 66486578 66487227 650 + 0.475 0.510 0.174
ENSG00000169032 E018 208.0390633 0.0002528056 4.175256e-01 5.608635e-01 15 66487228 66487292 65 + 2.293 2.343 0.167
ENSG00000169032 E019 16.6939582 0.0011040827 4.628214e-03 1.489714e-02 15 66487293 66489214 1922 + 1.352 1.150 -0.713
ENSG00000169032 E020 189.7929300 0.0015568306 2.140652e-01 3.433286e-01 15 66489215 66489276 62 + 2.245 2.311 0.222
ENSG00000169032 E021 3.8363630 0.0335876628 2.484999e-01 3.841655e-01 15 66489277 66489717 441 + 0.775 0.620 -0.646
ENSG00000169032 E022 169.8297108 0.0010291207 8.327312e-02 1.646909e-01 15 66489718 66489763 46 + 2.189 2.273 0.281
ENSG00000169032 E023 981.7920192 0.0028216407 2.477777e-10 4.081803e-09 15 66490502 66491656 1155 + 2.890 3.058 0.560