ENSG00000169016

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000381525 ENSG00000169016 HEK293_OSMI2_2hA HEK293_TMG_2hB E2F6 protein_coding protein_coding 17.59658 9.684805 26.92416 1.590418 0.7572376 1.474153 6.7404788 4.2888735 10.361648 0.6361202 0.6118907 1.270615 0.39700417 0.4589333 0.38430000 -0.07463333 7.335265e-01 2.462616e-30 FALSE TRUE
ENST00000421117 ENSG00000169016 HEK293_OSMI2_2hA HEK293_TMG_2hB E2F6 protein_coding nonsense_mediated_decay 17.59658 9.684805 26.92416 1.590418 0.7572376 1.474153 2.1478340 0.0000000 4.884529 0.0000000 0.3794635 8.935026 0.08526250 0.0000000 0.18090000 0.18090000 2.462616e-30 2.462616e-30 FALSE FALSE
ENST00000437573 ENSG00000169016 HEK293_OSMI2_2hA HEK293_TMG_2hB E2F6 protein_coding nonsense_mediated_decay 17.59658 9.684805 26.92416 1.590418 0.7572376 1.474153 1.2768081 0.2863652 2.393224 0.1437525 0.4751357 3.019523 0.05436667 0.0271000 0.08846667 0.06136667 1.897961e-01 2.462616e-30 FALSE TRUE
ENST00000455198 ENSG00000169016 HEK293_OSMI2_2hA HEK293_TMG_2hB E2F6 protein_coding nonsense_mediated_decay 17.59658 9.684805 26.92416 1.590418 0.7572376 1.474153 6.1468671 3.9083020 7.850440 1.0535078 0.6818550 1.004381 0.37136667 0.3906667 0.29306667 -0.09760000 5.365109e-01 2.462616e-30 FALSE TRUE
ENST00000546212 ENSG00000169016 HEK293_OSMI2_2hA HEK293_TMG_2hB E2F6 protein_coding protein_coding 17.59658 9.684805 26.92416 1.590418 0.7572376 1.474153 0.3531096 0.8869948 0.000000 0.1909587 0.0000000 -6.487028 0.03596250 0.0906000 0.00000000 -0.09060000 3.133618e-24 2.462616e-30 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000169016 E001 96.5032664 0.0098531907 1.615856e-01 2.771714e-01 2 11444375 11445299 925 - 1.942 2.032 0.301
ENSG00000169016 E002 290.0511823 0.0006566896 1.465991e-17 7.038437e-16 2 11445300 11446196 897 - 2.376 2.562 0.619
ENSG00000169016 E003 108.9661292 0.0016679101 6.100734e-01 7.294429e-01 2 11446197 11446476 280 - 2.022 2.045 0.076
ENSG00000169016 E004 47.0894065 0.0008079423 5.363724e-01 6.681317e-01 2 11446477 11446523 47 - 1.681 1.651 -0.099
ENSG00000169016 E005 139.2257437 0.0011777601 4.933509e-02 1.079773e-01 2 11447627 11447755 129 - 2.158 2.097 -0.205
ENSG00000169016 E006 85.7797491 0.0026881045 2.805527e-01 4.204030e-01 2 11447756 11447774 19 - 1.944 1.901 -0.144
ENSG00000169016 E007 107.2203013 0.0012766338 5.906707e-02 1.250274e-01 2 11450012 11450126 115 - 2.048 1.982 -0.223
ENSG00000169016 E008 5.7143833 0.0029461781 1.488031e-01 2.600462e-01 2 11451202 11451349 148 - 0.876 0.679 -0.788
ENSG00000169016 E009 4.3739398 0.0909000238 4.517301e-01 5.926599e-01 2 11451350 11451650 301 - 0.773 0.628 -0.600
ENSG00000169016 E010 113.9673414 0.0003188017 1.735337e-03 6.394199e-03 2 11451651 11451806 156 - 2.085 1.981 -0.349
ENSG00000169016 E011 160.8847874 0.0002784634 4.106619e-03 1.344207e-02 2 11453582 11453722 141 - 2.224 2.146 -0.262
ENSG00000169016 E012 119.1387875 0.0010401135 5.319777e-01 6.643616e-01 2 11453723 11453798 76 - 2.073 2.052 -0.070
ENSG00000169016 E013 14.2886110 0.0012018553 9.863297e-01 9.954833e-01 2 11455342 11455474 133 - 1.174 1.180 0.022
ENSG00000169016 E014 0.5889961 0.9385518299 6.029972e-01 7.238649e-01 2 11457011 11457178 168 - 0.278 0.000 -10.185
ENSG00000169016 E015 74.3395357 0.0202321109 9.804419e-01 9.917660e-01 2 11457179 11457233 55 - 1.866 1.848 -0.061
ENSG00000169016 E016 6.3612692 0.0414946747 2.350768e-01 3.684381e-01 2 11458281 11458351 71 - 0.914 0.720 -0.760
ENSG00000169016 E017 128.9947778 0.0043779868 5.308939e-02 1.146416e-01 2 11465772 11466177 406 - 2.130 2.039 -0.305