ENSG00000168887

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000306336 ENSG00000168887 HEK293_OSMI2_2hA HEK293_TMG_2hB C2orf68 protein_coding protein_coding 18.83538 25.93475 12.27872 1.020071 0.3414347 -1.078108 5.944600 6.987654 4.401469 0.5226789 0.23879028 -0.6656124 0.32845000 0.26970000 0.3583000 0.088600000 0.089216310 0.007808581 FALSE TRUE
ENST00000409734 ENSG00000168887 HEK293_OSMI2_2hA HEK293_TMG_2hB C2orf68 protein_coding protein_coding 18.83538 25.93475 12.27872 1.020071 0.3414347 -1.078108 1.967899 1.862098 1.592570 0.1641768 0.03130593 -0.2242693 0.11197083 0.07216667 0.1299667 0.057800000 0.007808581 0.007808581 FALSE FALSE
ENST00000420686 ENSG00000168887 HEK293_OSMI2_2hA HEK293_TMG_2hB C2orf68 protein_coding nonsense_mediated_decay 18.83538 25.93475 12.27872 1.020071 0.3414347 -1.078108 8.500064 14.028976 4.613127 1.1539134 0.11929281 -1.6024968 0.43647917 0.54116667 0.3768000 -0.164366667 0.036382214 0.007808581   FALSE
ENST00000478626 ENSG00000168887 HEK293_OSMI2_2hA HEK293_TMG_2hB C2orf68 protein_coding processed_transcript 18.83538 25.93475 12.27872 1.020071 0.3414347 -1.078108 1.786784 2.591075 1.280607 0.6581383 0.22965226 -1.0110581 0.08662917 0.09930000 0.1034667 0.004166667 0.952438063 0.007808581 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000168887 E001 453.60112 0.0094488442 5.937546e-02 1.255722e-01 2 85605254 85608283 3030 - 2.674 2.577 -0.323
ENSG00000168887 E002 206.73015 0.0003011620 1.534250e-05 9.769394e-05 2 85608284 85608566 283 - 2.197 2.286 0.298
ENSG00000168887 E003 341.55502 0.0002012855 6.621057e-09 8.441613e-08 2 85608567 85609067 501 - 2.416 2.505 0.296
ENSG00000168887 E004 166.10349 0.0014393191 8.557720e-01 9.108059e-01 2 85609435 85609586 152 - 2.183 2.161 -0.074
ENSG00000168887 E005 36.30602 0.0080690775 4.247838e-04 1.869315e-03 2 85610220 85611242 1023 - 1.701 1.421 -0.957
ENSG00000168887 E006 47.31497 0.0005249963 2.226011e-01 3.535028e-01 2 85611243 85611573 331 - 1.701 1.609 -0.312
ENSG00000168887 E007 18.93357 0.0010792955 1.143659e-01 2.119137e-01 2 85611574 85611667 94 - 1.355 1.200 -0.542
ENSG00000168887 E008 99.24368 0.0046665051 1.017442e-01 1.931550e-01 2 85611668 85611743 76 - 2.020 1.913 -0.359
ENSG00000168887 E009 86.62869 0.0059085562 3.065725e-01 4.487621e-01 2 85611744 85611786 43 - 1.947 1.865 -0.277
ENSG00000168887 E010 29.49396 0.0147255135 9.604204e-01 9.790666e-01 2 85611787 85611877 91 - 1.448 1.417 -0.108
ENSG00000168887 E011 104.03060 0.0135866928 9.734965e-01 9.874392e-01 2 85611878 85612066 189 - 1.986 1.957 -0.096