ENSG00000168615

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000487273 ENSG00000168615 HEK293_OSMI2_2hA HEK293_TMG_2hB ADAM9 protein_coding protein_coding 17.9339 4.148891 29.42706 0.2567283 0.614115 2.823363 15.58669954 3.2178961 25.63799028 0.04285664 0.62974682 2.990180 0.84211667 0.78146667 0.8713333333 0.08986667 0.2830117 0.0475352 FALSE TRUE
ENST00000676765 ENSG00000168615 HEK293_OSMI2_2hA HEK293_TMG_2hB ADAM9 protein_coding protein_coding 17.9339 4.148891 29.42706 0.2567283 0.614115 2.823363 0.85312310 0.3124926 1.36770332 0.17450494 0.06133449 2.094927 0.05690417 0.07393333 0.0464666667 -0.02746667 1.0000000 0.0475352 TRUE TRUE
ENST00000678863 ENSG00000168615 HEK293_OSMI2_2hA HEK293_TMG_2hB ADAM9 protein_coding nonsense_mediated_decay 17.9339 4.148891 29.42706 0.2567283 0.614115 2.823363 0.09140109 0.5162125 0.01561249 0.27479309 0.01561249 -4.360726 0.01575417 0.11950000 0.0005666667 -0.11893333 0.0475352 0.0475352 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000168615 E001 0.2955422 0.0292773515 1.525657e-01   8 38996754 38996765 12 + 0.047 0.286 3.035
ENSG00000168615 E002 5.4817889 0.0376591644 2.193633e-01 3.496036e-01 8 38996766 38996868 103 + 0.622 0.878 1.042
ENSG00000168615 E003 6.7707365 0.0133630570 1.148125e-01 2.125604e-01 8 38996869 38996913 45 + 0.687 0.974 1.123
ENSG00000168615 E004 8.5486725 0.0047233145 4.035431e-02 9.167109e-02 8 38996914 38996942 29 + 0.761 1.087 1.231
ENSG00000168615 E005 14.7261280 0.0015686836 8.938696e-01 9.361385e-01 8 38996943 38996972 30 + 1.023 1.086 0.230
ENSG00000168615 E006 14.1714630 0.0013219049 6.974317e-01 7.975658e-01 8 38996973 38996974 2 + 1.018 1.014 -0.016
ENSG00000168615 E007 22.1750170 0.0012951671 3.654408e-02 8.461253e-02 8 38996975 38996985 11 + 1.219 1.014 -0.739
ENSG00000168615 E008 48.4792300 0.0010342605 1.333094e-01 2.387193e-01 8 38996986 38997025 40 + 1.531 1.462 -0.236
ENSG00000168615 E009 79.2703429 0.0054729982 7.274746e-02 1.479225e-01 8 38997026 38997160 135 + 1.740 1.662 -0.264
ENSG00000168615 E010 0.8942170 0.0134731316 5.073960e-01 6.429441e-01 8 39006609 39007885 1277 + 0.226 0.000 -10.361
ENSG00000168615 E011 93.8333246 0.0003592692 1.358271e-04 6.849457e-04 8 39007886 39007983 98 + 1.823 1.642 -0.611
ENSG00000168615 E012 89.6899200 0.0002895266 1.011524e-04 5.271951e-04 8 39011658 39011716 59 + 1.805 1.614 -0.648
ENSG00000168615 E013 101.4704392 0.0022473985 1.463202e-02 3.966619e-02 8 39013965 39014043 79 + 1.850 1.755 -0.322
ENSG00000168615 E014 0.1472490 0.0433344799 7.564973e-01   8 39014044 39014491 448 + 0.047 0.000 -7.776
ENSG00000168615 E015 4.8683643 0.0033084312 3.419001e-03 1.149411e-02 8 39014492 39016117 1626 + 0.530 1.014 1.967
ENSG00000168615 E016 100.2129591 0.0002702943 2.366553e-03 8.357468e-03 8 39016118 39016194 77 + 1.847 1.726 -0.408
ENSG00000168615 E017 1.8340501 0.0123716835 6.417542e-01 7.547200e-01 8 39016195 39017218 1024 + 0.330 0.457 0.716
ENSG00000168615 E018 77.8253102 0.0004801313 6.915669e-02 1.419758e-01 8 39017219 39017272 54 + 1.732 1.669 -0.212
ENSG00000168615 E019 119.3682235 0.0003525063 2.599470e-03 9.062953e-03 8 39017273 39017414 142 + 1.920 1.815 -0.352
ENSG00000168615 E020 74.0167488 0.0003594199 3.303893e-03 1.116027e-02 8 39018853 39018881 29 + 1.718 1.573 -0.494
ENSG00000168615 E021 83.4537521 0.0003558335 7.225905e-03 2.180999e-02 8 39018882 39018918 37 + 1.767 1.652 -0.391
ENSG00000168615 E022 0.8909948 0.0136968632 7.350497e-01 8.258977e-01 8 39018919 39021642 2724 + 0.195 0.286 0.714
ENSG00000168615 E023 99.9522064 0.0005092474 2.043620e-03 7.361253e-03 8 39021643 39021714 72 + 1.845 1.718 -0.426
ENSG00000168615 E024 157.1999872 0.0002951350 4.093782e-06 2.969654e-05 8 39023156 39023325 170 + 2.042 1.879 -0.549
ENSG00000168615 E025 123.7433694 0.0002586420 2.919389e-04 1.346885e-03 8 39025803 39025884 82 + 1.938 1.803 -0.455
ENSG00000168615 E026 139.2117894 0.0018853056 2.465275e-03 8.660096e-03 8 39026677 39026810 134 + 1.985 1.879 -0.356
ENSG00000168615 E027 0.0000000       8 39032718 39032836 119 +      
ENSG00000168615 E028 143.3089790 0.0002379371 8.639009e-01 9.162487e-01 8 39041946 39042117 172 + 1.976 2.034 0.195
ENSG00000168615 E029 0.0000000       8 39046002 39046058 57 +      
ENSG00000168615 E030 0.0000000       8 39046954 39047046 93 +      
ENSG00000168615 E031 110.2067028 0.0002736556 2.544652e-01 3.911531e-01 8 39054481 39054573 93 + 1.857 1.961 0.352
ENSG00000168615 E032 174.8924581 0.0002584938 9.756875e-01 9.888304e-01 8 39055577 39055772 196 + 2.064 2.116 0.175
ENSG00000168615 E033 140.9708031 0.0047148555 5.383552e-01 6.697824e-01 8 39071298 39071403 106 + 1.977 1.995 0.059
ENSG00000168615 E034 0.0000000       8 39071404 39071413 10 +      
ENSG00000168615 E035 0.8921407 0.0131641375 5.077823e-01 6.432185e-01 8 39072937 39076146 3210 + 0.226 0.000 -10.361
ENSG00000168615 E036 1.4695947 0.0389911056 4.040797e-01 5.477877e-01 8 39076147 39076275 129 + 0.254 0.459 1.239
ENSG00000168615 E037 190.4539630 0.0011617593 9.444481e-01 9.691728e-01 8 39077228 39077411 184 + 2.101 2.155 0.180
ENSG00000168615 E038 139.3670152 0.0003156613 7.610446e-01 8.449841e-01 8 39082641 39082721 81 + 1.963 2.027 0.214
ENSG00000168615 E039 163.6992409 0.0004486713 7.975967e-01 8.708665e-01 8 39082968 39083073 106 + 2.033 2.094 0.205
ENSG00000168615 E040 127.5485262 0.0003208366 5.862837e-01 7.098675e-01 8 39090047 39090135 89 + 1.931 1.957 0.085
ENSG00000168615 E041 121.4404788 0.0002606542 5.505404e-01 6.801541e-01 8 39090136 39090188 53 + 1.911 1.934 0.075
ENSG00000168615 E042 5.1508729 0.0146898991 3.233522e-01 4.666750e-01 8 39090189 39091242 1054 + 0.609 0.820 0.869
ENSG00000168615 E043 0.5911862 0.0172671820 1.000000e+00 1.000000e+00 8 39091243 39091258 16 + 0.163 0.000 -9.776
ENSG00000168615 E044 81.6914482 0.0004180885 7.816797e-01 8.597406e-01 8 39091259 39091263 5 + 1.742 1.776 0.116
ENSG00000168615 E045 127.7401922 0.0004045530 8.079353e-01 8.779556e-01 8 39091264 39091346 83 + 1.929 1.991 0.208
ENSG00000168615 E046 0.4439371 0.0215614259 1.000000e+00 1.000000e+00 8 39091672 39091764 93 + 0.127 0.000 -9.361
ENSG00000168615 E047 0.7500885 0.1226257756 6.075338e-01 7.274837e-01 8 39100456 39101862 1407 + 0.163 0.285 1.023
ENSG00000168615 E048 105.4567348 0.0003038700 2.780870e-01 4.177124e-01 8 39101863 39101930 68 + 1.840 1.943 0.347
ENSG00000168615 E049 583.9297028 0.0083690378 5.720487e-11 1.051450e-09 8 39103607 39105445 1839 + 2.536 2.857 1.066