ENSG00000168566

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000342415 ENSG00000168566 HEK293_OSMI2_2hA HEK293_TMG_2hB SNRNP48 protein_coding protein_coding 25.83861 6.326031 47.25024 0.1701356 1.290056 2.898976 14.97283 5.725629 23.37535 0.4869281 0.4187755 2.027582 0.7043917 0.9021667 0.4952667 -0.4069000 3.284899e-04 7.688589e-36 FALSE TRUE
ENST00000634363 ENSG00000168566 HEK293_OSMI2_2hA HEK293_TMG_2hB SNRNP48 protein_coding nonsense_mediated_decay 25.83861 6.326031 47.25024 0.1701356 1.290056 2.898976 8.42425 0.000000 18.07091 0.0000000 1.1486303 10.820252 0.2041917 0.0000000 0.3823333 0.3823333 7.688589e-36 7.688589e-36 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000168566 E001 0.599719 0.1335037464 5.844649e-01 7.083792e-01 6 7590198 7590202 5 + 0.191 0.001 -8.297
ENSG00000168566 E002 1.812280 0.0078085328 3.959535e-01 5.397423e-01 6 7590203 7590208 6 + 0.400 0.248 -0.975
ENSG00000168566 E003 5.618317 0.0260522131 9.205051e-01 9.538305e-01 6 7590209 7590223 15 + 0.721 0.749 0.112
ENSG00000168566 E004 5.917081 0.0309787414 8.222799e-01 8.879449e-01 6 7590224 7590225 2 + 0.744 0.749 0.021
ENSG00000168566 E005 87.675522 0.0042561415 3.790299e-03 1.255480e-02 6 7590226 7590350 125 + 1.851 1.728 -0.416
ENSG00000168566 E006 97.292343 0.0134643284 1.517437e-01 2.640331e-01 6 7590351 7590413 63 + 1.885 1.831 -0.180
ENSG00000168566 E007 161.534454 0.0011466660 1.699351e-04 8.348123e-04 6 7593734 7593847 114 + 2.110 2.011 -0.332
ENSG00000168566 E008 141.144628 0.0002860794 1.084353e-01 2.032285e-01 6 7594099 7594159 61 + 2.036 2.027 -0.028
ENSG00000168566 E009 155.956653 0.0002728434 1.642047e-02 4.365690e-02 6 7595027 7595101 75 + 2.085 2.049 -0.120
ENSG00000168566 E010 9.038207 0.0019161680 5.660382e-02 1.207620e-01 6 7599673 7599680 8 + 0.933 0.686 -0.975
ENSG00000168566 E011 50.447184 0.0008545682 1.296036e-06 1.050756e-05 6 7599681 7601335 1655 + 1.640 1.322 -1.092
ENSG00000168566 E012 253.642941 0.0001655404 3.725003e-03 1.236969e-02 6 7601336 7601524 189 + 2.295 2.265 -0.098
ENSG00000168566 E013 265.712743 0.0002068834 5.035709e-05 2.835994e-04 6 7602623 7602744 122 + 2.320 2.258 -0.208
ENSG00000168566 E014 185.490278 0.0002415588 2.519054e-03 8.820228e-03 6 7605398 7605486 89 + 2.163 2.113 -0.168
ENSG00000168566 E015 235.162496 0.0010535913 7.176168e-03 2.168198e-02 6 7606031 7606195 165 + 2.263 2.227 -0.119
ENSG00000168566 E016 1254.748625 0.0019300597 1.958726e-17 9.221541e-16 6 7608825 7611967 3143 + 2.945 3.125 0.599