ENSG00000168495

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000397802 ENSG00000168495 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR3D protein_coding protein_coding 35.42829 50.7984 26.80852 2.340072 0.711537 -0.9218377 5.256371 7.991919 3.869075 0.7398676 0.3603232 -1.0446332 0.14625417 0.15830000 0.1452000 -0.01310000 0.87484198 0.02441954 FALSE  
ENST00000517789 ENSG00000168495 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR3D protein_coding retained_intron 35.42829 50.7984 26.80852 2.340072 0.711537 -0.9218377 21.616504 32.570627 14.740528 0.9687543 0.2792079 -1.1432478 0.61128750 0.64220000 0.5505667 -0.09163333 0.02441954 0.02441954 FALSE  
ENST00000519237 ENSG00000168495 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR3D protein_coding protein_coding 35.42829 50.7984 26.80852 2.340072 0.711537 -0.9218377 4.488126 4.887858 4.511612 1.5150455 0.9127276 -0.1153138 0.12460833 0.09390000 0.1669333 0.07303333 0.38779052 0.02441954 FALSE  
MSTRG.31246.1 ENSG00000168495 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR3D protein_coding   35.42829 50.7984 26.80852 2.340072 0.711537 -0.9218377 3.338338 4.220206 3.036209 0.6228481 0.3352831 -0.4737134 0.09442083 0.08373333 0.1140000 0.03026667 0.52829152 0.02441954 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000168495 E001 2.069009 0.0720121453 1.587011e-01 0.2733973282 8 22244964 22245132 169 + 0.639 0.366 -1.342
ENSG00000168495 E002 2.883717 0.1539749506 1.041962e-01 0.1968865830 8 22245133 22245143 11 + 0.797 0.406 -1.766
ENSG00000168495 E003 13.174032 0.0191253157 7.862032e-04 0.0032097891 8 22245144 22245165 22 + 1.334 0.957 -1.354
ENSG00000168495 E004 19.019786 0.0115126032 1.115139e-03 0.0043540788 8 22245166 22245183 18 + 1.444 1.138 -1.073
ENSG00000168495 E005 0.742699 0.0358083796 7.760814e-01 0.8558240652 8 22245184 22245220 37 + 0.170 0.222 0.477
ENSG00000168495 E006 38.418039 0.0037236435 8.108175e-01 0.8799734063 8 22245235 22245431 197 + 1.538 1.551 0.046
ENSG00000168495 E007 46.547177 0.0038646201 8.534838e-01 0.9093257946 8 22245432 22245444 13 + 1.639 1.625 -0.047
ENSG00000168495 E008 172.964229 0.0045772271 5.377911e-02 0.1158682051 8 22245445 22245614 170 + 2.249 2.165 -0.280
ENSG00000168495 E009 132.764791 0.0002934979 9.468087e-04 0.0037775640 8 22247221 22247264 44 + 2.154 2.045 -0.367
ENSG00000168495 E010 271.078751 0.0002438922 6.939465e-05 0.0003770934 8 22247857 22248008 152 + 2.451 2.358 -0.311
ENSG00000168495 E011 304.516679 0.0002361546 8.300505e-01 0.8933169243 8 22248154 22248278 125 + 2.441 2.434 -0.023
ENSG00000168495 E012 387.853527 0.0002445470 9.906551e-03 0.0285331246 8 22248481 22248649 169 + 2.504 2.553 0.163
ENSG00000168495 E013 512.782962 0.0001411503 1.627567e-05 0.0001030368 8 22249044 22249309 266 + 2.610 2.681 0.234
ENSG00000168495 E014 350.155811 0.0006775448 2.915778e-04 0.0013455094 8 22250075 22250227 153 + 2.438 2.520 0.274
ENSG00000168495 E015 1105.894351 0.0016982909 7.266821e-01 0.8196215552 8 22250396 22253938 3543 + 3.002 2.994 -0.026
ENSG00000168495 E016 94.369014 0.0003357033 3.348156e-01 0.4785478875 8 22253939 22254601 663 + 1.959 1.919 -0.134
ENSG00000168495 E017 4.643885 0.0109895228 9.552314e-05 0.0005010018 8 22255170 22255241 72 + 1.024 0.476 -2.261