ENSG00000168476

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000306306 ENSG00000168476 HEK293_OSMI2_2hA HEK293_TMG_2hB REEP4 protein_coding protein_coding 47.73177 69.78507 33.56888 3.867293 0.4290123 -1.055571 34.700685 52.042401 22.986905 2.7183939 0.4465301 -1.1785250 0.7181458 0.7467 0.6849667 -0.06173333 0.307200772 0.001957146 FALSE  
ENST00000334530 ENSG00000168476 HEK293_OSMI2_2hA HEK293_TMG_2hB REEP4 protein_coding protein_coding 47.73177 69.78507 33.56888 3.867293 0.4290123 -1.055571 5.794865 10.354332 5.053099 0.4035405 0.3181372 -1.0335344 0.1201083 0.1487 0.1504000 0.00170000 0.991651729 0.001957146 TRUE  
ENST00000519074 ENSG00000168476 HEK293_OSMI2_2hA HEK293_TMG_2hB REEP4 protein_coding retained_intron 47.73177 69.78507 33.56888 3.867293 0.4290123 -1.055571 4.747607 3.857761 3.886220 0.2114677 0.4747209 0.0105767 0.1095042 0.0558 0.1155000 0.05970000 0.001957146 0.001957146 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000168476 E001 276.467459 0.0060118113 2.195095e-01 3.498071e-01 8 22138020 22138160 141 - 2.345 2.398 0.177
ENSG00000168476 E002 395.581917 0.0032137707 4.881615e-02 1.070540e-01 8 22138161 22138250 90 - 2.492 2.555 0.212
ENSG00000168476 E003 701.404076 0.0012197327 1.092234e-04 5.644398e-04 8 22138251 22138552 302 - 2.726 2.806 0.265
ENSG00000168476 E004 451.332456 0.0001242162 4.486069e-06 3.228753e-05 8 22138639 22138793 155 - 2.529 2.613 0.278
ENSG00000168476 E005 7.007272 0.0427806214 7.892980e-02 1.578088e-01 8 22138903 22138906 4 - 1.025 0.755 -1.035
ENSG00000168476 E006 8.848503 0.0064688974 1.918431e-01 3.160770e-01 8 22138907 22138925 19 - 1.045 0.888 -0.588
ENSG00000168476 E007 362.706250 0.0002120230 3.210957e-03 1.088789e-02 8 22138926 22139055 130 - 2.452 2.511 0.199
ENSG00000168476 E008 149.336844 0.0002918902 1.923740e-02 4.984842e-02 8 22139056 22139061 6 - 2.055 2.130 0.250
ENSG00000168476 E009 65.446907 0.0045423493 3.752819e-05 2.177987e-04 8 22139062 22139415 354 - 1.923 1.695 -0.771
ENSG00000168476 E010 471.883860 0.0012897533 9.505899e-01 9.730512e-01 8 22139416 22139529 114 - 2.615 2.611 -0.013
ENSG00000168476 E011 15.148656 0.0406862741 4.196623e-05 2.406871e-04 8 22139802 22139962 161 - 1.475 0.957 -1.842
ENSG00000168476 E012 484.079108 0.0001888840 9.306550e-01 9.603650e-01 8 22139963 22140064 102 - 2.625 2.622 -0.009
ENSG00000168476 E013 278.345042 0.0001740210 2.650620e-01 4.030824e-01 8 22140065 22140083 19 - 2.367 2.390 0.075
ENSG00000168476 E014 368.019408 0.0001447112 2.733199e-01 4.123338e-01 8 22140172 22140205 34 - 2.524 2.498 -0.089
ENSG00000168476 E015 399.443893 0.0001361900 2.263236e-02 5.698808e-02 8 22140206 22140248 43 - 2.575 2.527 -0.161
ENSG00000168476 E016 11.065817 0.0445572847 6.507100e-04 2.718364e-03 8 22140607 22140624 18 - 1.327 0.848 -1.742
ENSG00000168476 E017 398.195952 0.0002241307 2.439673e-04 1.149923e-03 8 22140625 22140697 73 - 2.594 2.518 -0.254
ENSG00000168476 E018 494.909334 0.0029996307 7.960311e-04 3.245437e-03 8 22141451 22141659 209 - 2.707 2.606 -0.334
ENSG00000168476 E019 247.743198 0.0110706987 7.932140e-02 1.584379e-01 8 22141660 22141884 225 - 2.402 2.309 -0.311
ENSG00000168476 E020 50.082172 0.0125103089 3.166177e-01 4.594089e-01 8 22141885 22141951 67 - 1.699 1.623 -0.257