ENSG00000168461

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000578734 ENSG00000168461 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB31 protein_coding nonsense_mediated_decay 8.970186 7.928633 13.95207 1.21402 0.4242294 0.8145505 0.7040826 0.1770091 0.9746621 0.1770091 0.308194240 2.3965203 0.07640000 0.01760000 0.06866667 0.05106667 2.389441e-01 5.707122e-06 FALSE TRUE
ENST00000578921 ENSG00000168461 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB31 protein_coding protein_coding 8.970186 7.928633 13.95207 1.21402 0.4242294 0.8145505 7.1997382 6.4028889 12.6280398 0.3804057 0.006500913 0.9787264 0.80228333 0.83686667 0.90680000 0.06993333 8.995751e-01 5.707122e-06 FALSE TRUE
ENST00000580795 ENSG00000168461 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB31 protein_coding processed_transcript 8.970186 7.928633 13.95207 1.21402 0.4242294 0.8145505 0.2714544 0.8508197 0.0000000 0.8508197 0.000000000 -6.4276392 0.03234583 0.08460000 0.00000000 -0.08460000 6.872139e-01 5.707122e-06   FALSE
MSTRG.15487.2 ENSG00000168461 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB31 protein_coding   8.970186 7.928633 13.95207 1.21402 0.4242294 0.8145505 0.5974303 0.4979152 0.0000000 0.2046477 0.000000000 -5.6665159 0.06590417 0.06093333 0.00000000 -0.06093333 5.707122e-06 5.707122e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000168461 E001 4.4621855 0.0037115969 9.673279e-05 0.0005067678 18 9708275 9708298 24 + 0.869 0.285 -2.788
ENSG00000168461 E002 4.3514681 0.0035559088 1.018537e-03 0.0040226566 18 9708299 9708300 2 + 0.841 0.378 -2.096
ENSG00000168461 E003 4.6846879 0.0034501905 2.469520e-03 0.0086727565 18 9708301 9708306 6 + 0.856 0.455 -1.735
ENSG00000168461 E004 9.8628035 0.0149007138 5.888089e-02 0.1247155542 18 9708307 9708322 16 + 1.078 0.924 -0.572
ENSG00000168461 E005 38.2417537 0.0005385467 2.893261e-05 0.0001728103 18 9708323 9708444 122 + 1.639 1.473 -0.566
ENSG00000168461 E006 0.2998086 0.0283487501 7.111812e-01   18 9722742 9722863 122 + 0.089 0.165 1.017
ENSG00000168461 E007 0.0000000       18 9723558 9723713 156 +      
ENSG00000168461 E008 0.0000000       18 9726027 9726151 125 +      
ENSG00000168461 E009 0.0000000       18 9757963 9758151 189 +      
ENSG00000168461 E010 29.6557007 0.0006646164 3.337114e-03 0.0112544913 18 9775278 9775286 9 + 1.512 1.400 -0.389
ENSG00000168461 E011 56.9253481 0.0063223618 1.318646e-02 0.0363428558 18 9775287 9775357 71 + 1.775 1.700 -0.252
ENSG00000168461 E012 0.3030308 0.3942490937 5.112410e-01   18 9792153 9792153 1 + 0.164 0.000 -13.955
ENSG00000168461 E013 75.7828077 0.0173972489 6.430017e-02 0.1338682392 18 9792154 9792235 82 + 1.894 1.833 -0.206
ENSG00000168461 E014 0.0000000       18 9797359 9797507 149 +      
ENSG00000168461 E015 56.1922126 0.0148515855 1.294060e-01 0.2333515984 18 9814020 9814047 28 + 1.758 1.722 -0.123
ENSG00000168461 E016 55.2508392 0.0057247106 1.316851e-01 0.2364856168 18 9814048 9814091 44 + 1.742 1.730 -0.039
ENSG00000168461 E017 0.0000000       18 9814654 9814720 67 +      
ENSG00000168461 E018 0.0000000       18 9814721 9814730 10 +      
ENSG00000168461 E019 0.0000000       18 9814731 9814787 57 +      
ENSG00000168461 E020 65.2783661 0.0004029556 8.967459e-01 0.9380256659 18 9815116 9815222 107 + 1.783 1.862 0.267
ENSG00000168461 E021 0.1451727 0.0438642711 8.190413e-01   18 9815223 9815438 216 + 0.089 0.000 -12.947
ENSG00000168461 E022 0.1451727 0.0438642711 8.190413e-01   18 9816093 9816158 66 + 0.089 0.000 -12.947
ENSG00000168461 E023 73.8679102 0.0011040248 4.774358e-01 0.6159817225 18 9845582 9845691 110 + 1.817 1.931 0.382
ENSG00000168461 E024 1.5542982 0.1380038571 3.327764e-01 0.4764580211 18 9845692 9845692 1 + 0.282 0.524 1.358
ENSG00000168461 E025 1.2417286 0.1013082849 1.654436e-02 0.0439380745 18 9845874 9845941 68 + 0.089 0.579 3.613
ENSG00000168461 E026 0.0000000       18 9849596 9849656 61 +      
ENSG00000168461 E027 0.2987644 0.0275376561 3.918000e-01   18 9856044 9856354 311 + 0.164 0.000 -13.948
ENSG00000168461 E028 907.8773774 0.0146019482 1.327494e-02 0.0365585388 18 9859228 9862551 3324 + 2.892 3.021 0.430