ENSG00000168397

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000404914 ENSG00000168397 HEK293_OSMI2_2hA HEK293_TMG_2hB ATG4B protein_coding protein_coding 144.9303 213.6185 102.12 16.59784 3.268285 -1.064697 32.21067 51.85520 20.53879 2.553267 0.7170258 -1.3357138 0.2154375 0.2442333 0.2012667 -0.042966667 0.111314678 1.554151e-05 FALSE  
ENST00000428861 ENSG00000168397 HEK293_OSMI2_2hA HEK293_TMG_2hB ATG4B protein_coding retained_intron 144.9303 213.6185 102.12 16.59784 3.268285 -1.064697 24.22969 43.66575 13.90254 2.305795 1.5105330 -1.6504463 0.1574625 0.2052667 0.1356333 -0.069633333 0.003311495 1.554151e-05 FALSE  
ENST00000429899 ENSG00000168397 HEK293_OSMI2_2hA HEK293_TMG_2hB ATG4B protein_coding retained_intron 144.9303 213.6185 102.12 16.59784 3.268285 -1.064697 24.32175 33.55980 15.69517 4.434988 0.3737713 -1.0959247 0.1707333 0.1561000 0.1538333 -0.002266667 0.993639967 1.554151e-05 FALSE  
ENST00000465399 ENSG00000168397 HEK293_OSMI2_2hA HEK293_TMG_2hB ATG4B protein_coding retained_intron 144.9303 213.6185 102.12 16.59784 3.268285 -1.064697 33.43118 48.61748 27.75676 4.472856 2.3160554 -0.8084132 0.2345458 0.2271333 0.2715667 0.044433333 0.290116123 1.554151e-05 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000168397 E001 1.8704906 2.662117e-02 9.438842e-01 9.688092e-01 2 241637213 241637416 204 + 0.408 0.420 0.068
ENSG00000168397 E002 2.2704179 1.756630e-02 2.847899e-01 4.250545e-01 2 241637417 241637611 195 + 0.309 0.511 1.113
ENSG00000168397 E003 0.7718438 3.361823e-02 8.950684e-01 9.369462e-01 2 241637612 241637619 8 + 0.182 0.208 0.245
ENSG00000168397 E004 1.0653097 1.219891e-02 7.902855e-01 8.658413e-01 2 241637620 241637625 6 + 0.309 0.259 -0.346
ENSG00000168397 E005 1.5091451 9.268283e-03 7.432786e-01 8.319556e-01 2 241637626 241637634 9 + 0.408 0.347 -0.348
ENSG00000168397 E006 7.7137896 2.822888e-02 7.983387e-01 8.712950e-01 2 241637635 241637661 27 + 0.859 0.898 0.149
ENSG00000168397 E007 21.8812135 1.467103e-03 7.767901e-01 8.562816e-01 2 241637662 241637692 31 + 1.283 1.305 0.078
ENSG00000168397 E008 26.0296717 9.886320e-04 7.222952e-01 8.164430e-01 2 241637693 241637696 4 + 1.348 1.374 0.090
ENSG00000168397 E009 27.6098038 1.275958e-03 5.415202e-01 6.724924e-01 2 241637697 241637697 1 + 1.358 1.403 0.156
ENSG00000168397 E010 28.8599384 1.509781e-03 4.469421e-01 5.883871e-01 2 241637698 241637699 2 + 1.368 1.423 0.193
ENSG00000168397 E011 126.3594414 2.427413e-04 7.672062e-16 2.943396e-14 2 241637700 241637705 6 + 2.222 1.953 -0.899
ENSG00000168397 E012 136.3921504 2.255915e-04 3.333682e-16 1.343165e-14 2 241637706 241637706 1 + 2.250 1.988 -0.878
ENSG00000168397 E013 241.4617057 1.910801e-04 3.715814e-29 5.808166e-27 2 241637707 241637709 3 + 2.503 2.231 -0.910
ENSG00000168397 E014 439.3664944 1.286982e-03 5.906685e-02 1.250274e-01 2 241637710 241637724 15 + 2.612 2.565 -0.157
ENSG00000168397 E015 0.0000000       2 241637729 241637760 32 +      
ENSG00000168397 E016 0.2955422 2.958099e-02 4.967090e-01   2 241638261 241638370 110 + 0.182 0.080 -1.350
ENSG00000168397 E017 2.3896404 6.648377e-03 2.767352e-01 4.162166e-01 2 241651007 241651009 3 + 0.309 0.512 1.114
ENSG00000168397 E018 685.8270303 1.292629e-03 1.059353e-01 1.995221e-01 2 241651010 241651111 102 + 2.796 2.761 -0.116
ENSG00000168397 E019 5.7542574 2.632916e-02 3.098660e-03 1.055604e-02 2 241651112 241651263 152 + 1.036 0.612 -1.673
ENSG00000168397 E020 715.4259613 2.654140e-04 1.674667e-01 2.849067e-01 2 241651264 241651335 72 + 2.803 2.780 -0.076
ENSG00000168397 E021 10.8035368 2.325568e-03 2.481420e-10 4.087450e-09 2 241651884 241651974 91 + 1.387 0.755 -2.316
ENSG00000168397 E022 5.0314088 3.211307e-03 2.672185e-08 3.037954e-07 2 241651975 241652012 38 + 1.145 0.420 -2.992
ENSG00000168397 E023 33.2538407 1.132530e-02 9.651374e-03 2.790669e-02 2 241653307 241653369 63 + 1.607 1.402 -0.704
ENSG00000168397 E024 6.7922185 2.654895e-03 5.638490e-01 6.912497e-01 2 241653467 241653511 45 + 0.889 0.813 -0.293
ENSG00000168397 E025 710.9800849 1.024241e-04 4.558925e-01 5.963863e-01 2 241653512 241653610 99 + 2.775 2.786 0.034
ENSG00000168397 E026 777.0807152 1.086709e-03 1.079382e-01 2.024730e-01 2 241654546 241654647 102 + 2.796 2.831 0.118
ENSG00000168397 E027 27.8091325 7.957967e-04 2.377600e-05 1.449490e-04 2 241654648 241655002 355 + 1.590 1.301 -0.998
ENSG00000168397 E028 628.7441688 2.413103e-04 7.180079e-02 1.463464e-01 2 241655271 241655343 73 + 2.706 2.737 0.102
ENSG00000168397 E029 7.2139961 3.697378e-03 5.545916e-03 1.740738e-02 2 241655344 241655444 101 + 1.076 0.740 -1.279
ENSG00000168397 E030 585.6235964 9.900715e-04 6.335151e-02 1.322799e-01 2 241659108 241659187 80 + 2.670 2.711 0.138
ENSG00000168397 E031 38.3011409 8.822158e-03 2.124479e-03 7.609567e-03 2 241659188 241659772 585 + 1.327 1.586 0.890
ENSG00000168397 E032 0.9587465 2.847452e-02 7.870811e-01 8.636657e-01 2 241666596 241666644 49 + 0.309 0.259 -0.351
ENSG00000168397 E033 962.4548297 1.121384e-04 4.092855e-03 1.340257e-02 2 241666645 241666838 194 + 2.886 2.924 0.125
ENSG00000168397 E034 16.9580181 8.000111e-03 1.729755e-08 2.040835e-07 2 241667812 241668142 331 + 1.507 0.992 -1.821
ENSG00000168397 E035 368.6147966 5.927777e-04 8.431644e-04 3.412849e-03 2 241668143 241668168 26 + 2.439 2.520 0.267
ENSG00000168397 E036 492.3253321 7.249154e-04 1.519420e-04 7.556098e-04 2 241668169 241668221 53 + 2.561 2.645 0.282
ENSG00000168397 E037 16.5526621 1.499196e-02 1.546560e-12 3.672028e-11 2 241668259 241668539 281 + 1.578 0.869 -2.527
ENSG00000168397 E038 801.1731188 9.775552e-05 1.368749e-08 1.644921e-07 2 241668540 241668685 146 + 2.771 2.854 0.277
ENSG00000168397 E039 23.9191668 8.216866e-04 1.707357e-07 1.658736e-06 2 241668686 241668860 175 + 1.578 1.201 -1.307
ENSG00000168397 E040 26.2357316 1.832012e-03 1.158125e-15 4.335176e-14 2 241670561 241670725 165 + 1.711 1.154 -1.924
ENSG00000168397 E041 563.7785435 5.965357e-04 2.701198e-04 1.258166e-03 2 241670726 241670782 57 + 2.626 2.702 0.251
ENSG00000168397 E042 402.9012342 3.124996e-04 6.523067e-04 2.724073e-03 2 241671312 241671327 16 + 2.481 2.555 0.246
ENSG00000168397 E043 623.1302652 1.324234e-04 1.662804e-05 1.050145e-04 2 241671328 241671405 78 + 2.671 2.743 0.239
ENSG00000168397 E044 44.5821109 5.505527e-03 4.212848e-04 1.855900e-03 2 241671406 241671425 20 + 1.749 1.519 -0.780
ENSG00000168397 E045 35.8936660 1.105149e-02 8.396906e-04 3.400604e-03 2 241671426 241671441 16 + 1.674 1.416 -0.885
ENSG00000168397 E046 49.1139882 6.654874e-03 5.981873e-08 6.345172e-07 2 241671442 241671551 110 + 1.864 1.518 -1.174
ENSG00000168397 E047 121.0911695 7.679203e-03 2.791382e-11 5.406153e-10 2 241671552 241672190 639 + 2.258 1.902 -1.193
ENSG00000168397 E048 1716.3430616 7.556792e-04 1.589253e-01 2.737033e-01 2 241672191 241673857 1667 + 3.151 3.174 0.076