ENSG00000168273

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000307106 ENSG00000168273 HEK293_OSMI2_2hA HEK293_TMG_2hB SMIM4 protein_coding protein_coding 48.03112 84.68161 28.58881 1.910361 0.8080097 -1.566264 2.607172 4.065006 1.690006 1.479828 0.8853342 -1.261262 0.05732083 0.04726667 0.06093333 0.01366667 0.966927401 0.0004935083    
ENST00000476842 ENSG00000168273 HEK293_OSMI2_2hA HEK293_TMG_2hB SMIM4 protein_coding protein_coding 48.03112 84.68161 28.58881 1.910361 0.8080097 -1.566264 34.171794 62.452447 19.021387 1.968890 0.5759286 -1.714608 0.69842917 0.73720000 0.66526667 -0.07193333 0.001342657 0.0004935083    
ENST00000477703 ENSG00000168273 HEK293_OSMI2_2hA HEK293_TMG_2hB SMIM4 protein_coding protein_coding 48.03112 84.68161 28.58881 1.910361 0.8080097 -1.566264 5.184590 9.287187 2.140466 1.515514 1.3418696 -2.112145 0.10748750 0.11056667 0.07333333 -0.03723333 0.743427469 0.0004935083 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000168273 E001 81.919205 0.0003241507 4.491639e-04 1.963604e-03 3 52534013 52534148 136 + 1.939 1.785 -0.520
ENSG00000168273 E002 3.934489 0.0354568451 3.856631e-01 5.298482e-01 3 52534525 52534802 278 + 0.716 0.565 -0.652
ENSG00000168273 E003 73.218231 0.0003861019 8.309769e-05 4.426682e-04 3 52534803 52534877 75 + 1.911 1.730 -0.611
ENSG00000168273 E004 2.838687 0.0054103452 9.341051e-01 9.624847e-01 3 52536521 52536582 62 + 0.532 0.517 -0.070
ENSG00000168273 E005 5.375678 0.0032146184 4.210820e-01 5.642194e-01 3 52536583 52536601 19 + 0.807 0.688 -0.479
ENSG00000168273 E006 5.927121 0.0030389172 6.132186e-01 7.319792e-01 3 52536602 52536604 3 + 0.807 0.733 -0.294
ENSG00000168273 E007 839.378830 0.0001191703 7.889325e-05 4.227076e-04 3 52536605 52536773 169 + 2.783 2.832 0.165
ENSG00000168273 E008 688.628808 0.0002437404 2.948847e-03 1.011406e-02 3 52536774 52536906 133 + 2.700 2.745 0.148
ENSG00000168273 E009 570.586581 0.0001899328 4.458423e-01 5.873841e-01 3 52540424 52541890 1467 + 2.667 2.654 -0.043
ENSG00000168273 E010 0.147249 0.0428745961 1.332419e-01   3 52566361 52566386 26 + 0.204 0.000 -10.033
ENSG00000168273 E011 13.425491 0.0018284738 2.287269e-19 1.363487e-17 3 52579018 52579255 238 + 1.569 0.760 -2.922
ENSG00000168273 E012 10.619078 0.0016511438 4.327001e-17 1.942966e-15 3 52585208 52585511 304 + 1.483 0.655 -3.063