ENSG00000168209

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000307365 ENSG00000168209 HEK293_OSMI2_2hA HEK293_TMG_2hB DDIT4 protein_coding protein_coding 70.9761 91.03434 33.74353 4.700141 1.144792 -1.431531 67.84843 89.05447 30.70576 4.671295 0.6615803 -1.535871 0.9497542 0.9781667 0.9108667 -0.0673 5.108046e-07 5.108046e-07 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000168209 E001 0.5117906 2.425745e-01 1.804652e-01 3.017688e-01 10 72273919 72273919 1 + 0.330 0.075 -2.595
ENSG00000168209 E002 0.9149402 1.947219e-01 5.265023e-01 6.597325e-01 10 72273920 72273923 4 + 0.330 0.196 -0.997
ENSG00000168209 E003 349.3173881 1.361995e-03 1.768923e-01 2.972134e-01 10 72273924 72274060 137 + 2.479 2.447 -0.107
ENSG00000168209 E004 10.8871221 4.544853e-03 1.552277e-10 2.651468e-09 10 72274061 72274156 96 + 1.408 0.764 -2.352
ENSG00000168209 E005 393.2843262 1.177245e-03 1.312524e-02 3.619940e-02 10 72274157 72274224 68 + 2.552 2.493 -0.195
ENSG00000168209 E006 618.7704310 1.325705e-04 3.772941e-01 5.216256e-01 10 72274225 72274421 197 + 2.710 2.700 -0.034
ENSG00000168209 E007 13.4812650 1.368546e-03 4.909404e-06 3.500854e-05 10 72274422 72274694 273 + 1.367 0.944 -1.516
ENSG00000168209 E008 1901.2965243 8.406199e-05 5.390358e-02 1.160915e-01 10 72274695 72275559 865 + 3.198 3.188 -0.034
ENSG00000168209 E009 789.3902879 1.713518e-03 4.494102e-04 1.964499e-03 10 72275560 72275899 340 + 2.742 2.827 0.281
ENSG00000168209 E010 201.4443574 2.322902e-04 6.964299e-10 1.064520e-08 10 72275900 72276036 137 + 2.051 2.255 0.684