ENSG00000168159

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000305943 ENSG00000168159 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF187 protein_coding protein_coding 79.05959 166.0885 33.20148 4.423096 0.5349663 -2.322285 71.695612 142.609113 29.48369911 16.528789 0.67648850 -2.2736887 0.91002083 0.8553 0.8878333333 0.03253333 1.0000000000 0.0003746807 FALSE  
MSTRG.3203.2 ENSG00000168159 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF187 protein_coding   79.05959 166.0885 33.20148 4.423096 0.5349663 -2.322285 3.760939 4.561961 2.97973424 1.016208 0.26591134 -0.6127958 0.05146250 0.0272 0.0899666667 0.06276667 0.0003746807 0.0003746807 FALSE  
MSTRG.3203.3 ENSG00000168159 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF187 protein_coding   79.05959 166.0885 33.20148 4.423096 0.5349663 -2.322285 2.137226 15.272880 0.01257672 14.710571 0.01257672 -9.4028647 0.01825417 0.0957 0.0003666667 -0.09533333 0.5380135267 0.0003746807 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000168159 E001 10.707745 0.0593733263 8.557047e-01 9.107803e-01 1 228486510 228486907 398 + 0.847 0.913 0.253
ENSG00000168159 E002 16.410812 0.0541583663 2.580393e-01 3.952480e-01 1 228487002 228487131 130 + 0.890 1.101 0.779
ENSG00000168159 E003 13.564817 0.0019326688 2.503563e-01 3.863607e-01 1 228487132 228487272 141 + 0.847 1.018 0.647
ENSG00000168159 E004 4.974340 0.0400389169 4.791295e-01 6.175229e-01 1 228487273 228487327 55 + 0.513 0.675 0.724
ENSG00000168159 E005 94.059981 0.0262287943 1.273467e-06 1.034184e-05 1 228487328 228487381 54 + 2.133 1.728 -1.360
ENSG00000168159 E006 1550.680329 0.0111904528 1.344641e-12 3.228213e-11 1 228487382 228487878 497 + 3.293 2.962 -1.100
ENSG00000168159 E007 2.624631 0.1047927669 2.554393e-02 6.300412e-02 1 228488222 228488485 264 + 0.798 0.374 -1.951
ENSG00000168159 E008 869.621864 0.0034213160 7.895842e-06 5.370301e-05 1 228488960 228488968 9 + 2.877 2.753 -0.412
ENSG00000168159 E009 2309.774177 0.0012606406 3.319651e-03 1.120471e-02 1 228488969 228489052 84 + 3.227 3.191 -0.122
ENSG00000168159 E010 11.742525 0.0015224101 2.909640e-04 1.343086e-03 1 228489053 228489056 4 + 1.263 0.881 -1.391
ENSG00000168159 E011 7.064578 0.0903715460 8.720404e-02 1.709025e-01 1 228490709 228490760 52 + 1.002 0.702 -1.164
ENSG00000168159 E012 4672.116975 0.0002577365 8.032628e-02 1.600712e-01 1 228493053 228493274 222 + 3.471 3.506 0.115
ENSG00000168159 E013 16618.968466 0.0029038520 1.781332e-08 2.096205e-07 1 228493883 228499899 6017 + 3.983 4.063 0.269