ENSG00000168061

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000398846 ENSG00000168061 HEK293_OSMI2_2hA HEK293_TMG_2hB SAC3D1 protein_coding protein_coding 84.47865 158.071 50.04718 3.873599 0.480546 -1.659015 4.432908 6.481596 2.203321 1.045489 0.2955306 -1.5523606 0.05232917 0.0411000 0.04403333 0.002933333 0.92199233 0.01522533 FALSE TRUE
ENST00000533017 ENSG00000168061 HEK293_OSMI2_2hA HEK293_TMG_2hB SAC3D1 protein_coding processed_transcript 84.47865 158.071 50.04718 3.873599 0.480546 -1.659015 8.438323 16.557704 4.418350 1.145555 0.4911279 -1.9035326 0.09932500 0.1051667 0.08846667 -0.016700000 0.66256863 0.01522533   FALSE
ENST00000652489 ENSG00000168061 HEK293_OSMI2_2hA HEK293_TMG_2hB SAC3D1 protein_coding protein_coding 84.47865 158.071 50.04718 3.873599 0.480546 -1.659015 26.847401 38.565885 20.881729 5.231926 1.2247782 -0.8847673 0.30904167 0.2436667 0.41776667 0.174100000 0.01522533 0.01522533 FALSE TRUE
MSTRG.5695.2 ENSG00000168061 HEK293_OSMI2_2hA HEK293_TMG_2hB SAC3D1 protein_coding   84.47865 158.071 50.04718 3.873599 0.480546 -1.659015 41.552067 90.336608 19.741068 6.327722 1.6906315 -2.1935397 0.50257917 0.5715667 0.39390000 -0.177666667 0.02681985 0.01522533   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000168061 E001 4.192366 0.0039049703 0.3306952452 0.474295529 11 65040901 65040917 17 + 0.737 0.607 -0.551
ENSG00000168061 E002 19.024526 0.0145030477 0.5959467743 0.717955214 11 65040918 65040940 23 + 1.235 1.205 -0.103
ENSG00000168061 E003 61.201533 0.0004752396 0.1215281047 0.222110971 11 65040941 65040969 29 + 1.620 1.730 0.372
ENSG00000168061 E004 122.038039 0.0003213900 0.0294879317 0.070943821 11 65040970 65041106 137 + 1.916 2.026 0.370
ENSG00000168061 E005 76.163651 0.0032325130 0.0478303006 0.105321171 11 65041107 65041209 103 + 1.690 1.827 0.465
ENSG00000168061 E006 94.139098 0.0137199976 0.0055447731 0.017404368 11 65041210 65041244 35 + 2.013 1.854 -0.536
ENSG00000168061 E007 174.741164 0.0170275854 0.0032893696 0.011119142 11 65041245 65041285 41 + 2.284 2.120 -0.550
ENSG00000168061 E008 556.215484 0.0102679377 0.0002848208 0.001318182 11 65041286 65041866 581 + 2.758 2.632 -0.420
ENSG00000168061 E009 9.001681 0.0386242844 0.0578769190 0.122992715 11 65041867 65041928 62 + 1.104 0.862 -0.898
ENSG00000168061 E010 81.970236 0.0003517658 0.1745722098 0.294142280 11 65043025 65043164 140 + 1.863 1.826 -0.125
ENSG00000168061 E011 2006.006097 0.0132438066 0.0021294172 0.007625642 11 65044225 65044828 604 + 3.141 3.241 0.333