ENSG00000167553

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000301072 ENSG00000167553 HEK293_OSMI2_2hA HEK293_TMG_2hB TUBA1C protein_coding protein_coding 507.2431 843.1429 307.5578 46.0871 1.810004 -1.45489 317.7792 614.6667 161.5005 7.227592 0.9053729 -1.92820 0.5886250 0.7327333 0.5251667 -0.2075667 0.0008368854 0.0008368854 FALSE TRUE
ENST00000549183 ENSG00000167553 HEK293_OSMI2_2hA HEK293_TMG_2hB TUBA1C protein_coding protein_coding 507.2431 843.1429 307.5578 46.0871 1.810004 -1.45489 186.5757 225.9415 142.0237 41.138033 3.0303128 -0.66978 0.4040542 0.2640667 0.4617333 0.1976667 0.0027879031 0.0008368854 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000167553 E001 9.651165e+00 0.0457123697 3.556333e-08 3.945287e-07 12 49188736 49189130 395 + 1.404 0.654 -2.793
ENSG00000167553 E002 5.885377e+00 0.0259692846 5.338311e-09 6.929270e-08 12 49189131 49189225 95 + 1.241 0.463 -3.108
ENSG00000167553 E003 3.516970e+00 0.0605271771 5.351986e-02 1.154081e-01 12 49189694 49189720 27 + 0.813 0.490 -1.395
ENSG00000167553 E004 0.000000e+00       12 49208546 49208831 286 +      
ENSG00000167553 E005 1.472490e-01 0.0429717771 1.137572e-01   12 49208832 49208949 118 + 0.207 0.000 -10.395
ENSG00000167553 E006 0.000000e+00       12 49227926 49227926 1 +      
ENSG00000167553 E007 0.000000e+00       12 49227927 49227929 3 +      
ENSG00000167553 E008 0.000000e+00       12 49227930 49228042 113 +      
ENSG00000167553 E009 0.000000e+00       12 49228043 49228090 48 +      
ENSG00000167553 E010 2.955422e-01 0.0289176346 3.263321e-01   12 49228091 49228166 76 + 0.207 0.069 -1.813
ENSG00000167553 E011 4.772466e-01 0.0219646269 5.890906e-01 7.121890e-01 12 49256397 49256511 115 + 0.207 0.129 -0.812
ENSG00000167553 E012 0.000000e+00       12 49257884 49258001 118 +      
ENSG00000167553 E013 3.784932e+01 0.0005838599 1.230210e-03 4.742962e-03 12 49265081 49265081 1 + 1.622 1.451 -0.583
ENSG00000167553 E014 4.449067e+03 0.0011780424 2.025370e-09 2.830900e-08 12 49265082 49265184 103 + 3.588 3.532 -0.187
ENSG00000167553 E015 9.113222e+03 0.0006253379 1.550681e-10 2.649219e-09 12 49269465 49269687 223 + 3.875 3.850 -0.084
ENSG00000167553 E016 2.601921e+01 0.0277959260 2.147634e-01 3.441545e-01 12 49269786 49269827 42 + 1.405 1.304 -0.352
ENSG00000167553 E017 6.777937e+03 0.0004286350 3.037878e-03 1.037664e-02 12 49269828 49269976 149 + 3.716 3.728 0.040
ENSG00000167553 E018 2.640347e+01 0.0153685070 2.762797e-06 2.084858e-05 12 49269977 49270196 220 + 1.622 1.230 -1.356
ENSG00000167553 E019 2.102150e+04 0.0013223308 2.494329e-12 5.736140e-11 12 49272253 49274600 2348 + 4.127 4.237 0.364