ENSG00000167333

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000300747 ENSG00000167333 HEK293_OSMI2_2hA HEK293_TMG_2hB TRIM68 protein_coding protein_coding 7.625339 9.577964 6.049626 0.6145923 0.3346749 -0.6619959 6.1011573 8.3602267 4.4405878 0.50052042 0.2869250 -0.9112708 0.79318333 0.87336667 0.7332667 -0.14010000 2.611564e-05 2.611564e-05 FALSE TRUE
ENST00000531644 ENSG00000167333 HEK293_OSMI2_2hA HEK293_TMG_2hB TRIM68 protein_coding retained_intron 7.625339 9.577964 6.049626 0.6145923 0.3346749 -0.6619959 0.4158578 0.2373092 0.6256790 0.23730920 0.3159658 1.3619824 0.06135833 0.02243333 0.1036000 0.08116667 5.099397e-01 2.611564e-05   FALSE
ENST00000531717 ENSG00000167333 HEK293_OSMI2_2hA HEK293_TMG_2hB TRIM68 protein_coding retained_intron 7.625339 9.577964 6.049626 0.6145923 0.3346749 -0.6619959 0.6206203 0.5118881 0.7307096 0.04965431 0.1669324 0.5051676 0.08310833 0.05390000 0.1219333 0.06803333 8.782677e-02 2.611564e-05 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000167333 E001 25.138527 0.0092669369 1.064835e-03 4.183530e-03 11 4598672 4598779 108 - 1.143 1.458 1.103
ENSG00000167333 E002 416.896073 0.0009950159 6.500098e-08 6.846570e-07 11 4598780 4600638 1859 - 2.509 2.610 0.333
ENSG00000167333 E003 63.479018 0.0004519186 9.495482e-01 9.724069e-01 11 4600639 4600799 161 - 1.761 1.773 0.041
ENSG00000167333 E004 31.673176 0.0006606596 5.740410e-01 6.999045e-01 11 4600800 4600826 27 - 1.443 1.489 0.159
ENSG00000167333 E005 46.346291 0.0005005075 6.839144e-01 7.869961e-01 11 4601027 4601127 101 - 1.614 1.645 0.106
ENSG00000167333 E006 8.453891 0.0061094476 2.745108e-03 9.507359e-03 11 4601128 4601565 438 - 1.143 0.814 -1.228
ENSG00000167333 E007 3.094667 0.0061268103 2.756493e-02 6.714189e-02 11 4601641 4601663 23 - 0.783 0.453 -1.464
ENSG00000167333 E008 25.371922 0.0031989195 5.514272e-01 6.808945e-01 11 4601664 4601671 8 - 1.405 1.367 -0.131
ENSG00000167333 E009 24.714974 0.0008540548 5.098829e-01 6.451641e-01 11 4601672 4601686 15 - 1.398 1.358 -0.136
ENSG00000167333 E010 3.242894 0.0048043584 9.699398e-02 1.858862e-01 11 4601687 4602151 465 - 0.377 0.670 1.408
ENSG00000167333 E011 40.653931 0.0006073621 4.404679e-01 5.821428e-01 11 4602152 4602243 92 - 1.604 1.569 -0.120
ENSG00000167333 E012 49.053701 0.0005258793 2.432525e-02 6.048493e-02 11 4602244 4602406 163 - 1.733 1.627 -0.359
ENSG00000167333 E013 19.410845 0.0231445886 4.525137e-02 1.006509e-01 11 4602407 4602412 6 - 1.405 1.202 -0.710
ENSG00000167333 E014 1.653400 0.0216647589 2.793901e-01 4.191429e-01 11 4603241 4603244 4 - 0.519 0.332 -1.004
ENSG00000167333 E015 46.307344 0.0077688222 1.529711e-01 2.657099e-01 11 4603245 4603340 96 - 1.694 1.608 -0.293
ENSG00000167333 E016 83.582171 0.0007628906 1.396418e-05 8.971351e-05 11 4605079 4605561 483 - 1.995 1.833 -0.543
ENSG00000167333 E017 31.932991 0.0007144416 4.228280e-03 1.378053e-02 11 4608027 4608231 205 - 1.589 1.419 -0.581