ENSG00000167081

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000342287 ENSG00000167081 HEK293_OSMI2_2hA HEK293_TMG_2hB PBX3 protein_coding protein_coding 12.71933 12.94498 12.23459 0.9809632 0.8939938 -0.08136293 3.0362658 1.8241575 3.5124149 1.2861248 0.3868888 0.9414473 0.24112917 0.14576667 0.29090000 0.14513333 0.600519945 0.007053924 FALSE  
ENST00000373482 ENSG00000167081 HEK293_OSMI2_2hA HEK293_TMG_2hB PBX3 protein_coding nonsense_mediated_decay 12.71933 12.94498 12.23459 0.9809632 0.8939938 -0.08136293 1.1524480 1.2706745 0.7616370 1.2706745 0.7616370 -0.7309096 0.09082083 0.09846667 0.05906667 -0.03940000 1.000000000 0.007053924 FALSE  
ENST00000373489 ENSG00000167081 HEK293_OSMI2_2hA HEK293_TMG_2hB PBX3 protein_coding protein_coding 12.71933 12.94498 12.23459 0.9809632 0.8939938 -0.08136293 2.8564599 2.8998324 2.3533595 1.4959015 0.4696242 -0.3000970 0.22094583 0.22320000 0.19396667 -0.02923333 0.964156499 0.007053924 FALSE  
ENST00000373492 ENSG00000167081 HEK293_OSMI2_2hA HEK293_TMG_2hB PBX3 protein_coding nonsense_mediated_decay 12.71933 12.94498 12.23459 0.9809632 0.8939938 -0.08136293 1.4751106 2.7102587 1.1132585 1.4675021 0.5702747 -1.2760539 0.11436250 0.20596667 0.09340000 -0.11256667 0.895156828 0.007053924 FALSE  
ENST00000428092 ENSG00000167081 HEK293_OSMI2_2hA HEK293_TMG_2hB PBX3 protein_coding protein_coding 12.71933 12.94498 12.23459 0.9809632 0.8939938 -0.08136293 0.4717472 0.0000000 1.2521146 0.0000000 0.6274762 6.9796992 0.03193750 0.00000000 0.09540000 0.09540000 0.163190247 0.007053924 FALSE  
ENST00000492314 ENSG00000167081 HEK293_OSMI2_2hA HEK293_TMG_2hB PBX3 protein_coding retained_intron 12.71933 12.94498 12.23459 0.9809632 0.8939938 -0.08136293 1.5610573 2.4511587 1.0090988 0.5068609 0.1373697 -1.2720437 0.12495417 0.18616667 0.08516667 -0.10100000 0.065338062 0.007053924    
ENST00000497091 ENSG00000167081 HEK293_OSMI2_2hA HEK293_TMG_2hB PBX3 protein_coding processed_transcript 12.71933 12.94498 12.23459 0.9809632 0.8939938 -0.08136293 0.6884006 0.1978192 1.1770202 0.1001339 0.2701906 2.5139434 0.05636667 0.01450000 0.09440000 0.07990000 0.007053924 0.007053924 FALSE  
MSTRG.33394.2 ENSG00000167081 HEK293_OSMI2_2hA HEK293_TMG_2hB PBX3 protein_coding   12.71933 12.94498 12.23459 0.9809632 0.8939938 -0.08136293 0.8455308 0.7366698 0.5085501 0.2662280 0.1225411 -0.5259871 0.06756250 0.05953333 0.04073333 -0.01880000 0.837759218 0.007053924 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000167081 E001 3.0508581 0.1251508491 7.810481e-01 8.593203e-01 9 125747345 125747372 28 + 0.547 0.607 0.275
ENSG00000167081 E002 3.0434713 0.4548029725 9.539590e-01 9.751648e-01 9 125747373 125747373 1 + 0.587 0.591 0.016
ENSG00000167081 E003 15.6316597 0.0063625152 3.463000e-02 8.095246e-02 9 125747374 125747453 80 + 1.311 1.117 -0.688
ENSG00000167081 E004 70.1526896 0.0003946818 3.472486e-04 1.569154e-03 9 125747454 125747653 200 + 1.928 1.785 -0.482
ENSG00000167081 E005 0.1451727 0.0430538316 4.086399e-01   9 125748165 125748181 17 + 0.134 0.000 -10.423
ENSG00000167081 E006 0.2966881 0.0290785164 1.528205e-01   9 125748182 125748198 17 + 0.236 0.000 -11.551
ENSG00000167081 E007 0.8450104 0.0160176853 5.049086e-01 6.407737e-01 9 125748199 125748428 230 + 0.318 0.201 -0.879
ENSG00000167081 E008 0.5880631 0.3909298230 7.512538e-01 8.377270e-01 9 125748429 125748549 121 + 0.133 0.208 0.774
ENSG00000167081 E009 59.7988156 0.0045109592 5.820749e-02 1.235586e-01 9 125748550 125748623 74 + 1.837 1.742 -0.324
ENSG00000167081 E010 5.8184075 0.0029423013 2.161867e-02 5.488424e-02 9 125748931 125748944 14 + 0.628 0.936 1.234
ENSG00000167081 E011 33.9730004 0.0005974859 1.741215e-07 1.689032e-06 9 125748945 125749537 593 + 1.311 1.645 1.149
ENSG00000167081 E012 0.3268771 0.0292881245 8.828217e-01   9 125750474 125750510 37 + 0.134 0.112 -0.295
ENSG00000167081 E013 3.7701234 0.1128294397 5.471418e-03 1.721204e-02 9 125777982 125778103 122 + 0.236 0.823 2.970
ENSG00000167081 E014 1.1114209 0.1130916094 5.769062e-01 7.022583e-01 9 125785456 125785654 199 + 0.388 0.274 -0.718
ENSG00000167081 E015 2.5775898 0.0058563689 5.647494e-01 6.919908e-01 9 125797395 125797465 71 + 0.500 0.595 0.440
ENSG00000167081 E016 0.0000000       9 125843713 125843734 22 +      
ENSG00000167081 E017 0.0000000       9 125843735 125843812 78 +      
ENSG00000167081 E018 0.0000000       9 125844566 125844982 417 +      
ENSG00000167081 E019 0.0000000       9 125865271 125865305 35 +      
ENSG00000167081 E020 60.6226768 0.0004305940 4.208714e-08 4.609367e-07 9 125915686 125915927 242 + 1.911 1.673 -0.803
ENSG00000167081 E021 85.5044954 0.0007317020 1.365228e-04 6.880876e-04 9 125929655 125929845 191 + 2.008 1.865 -0.482
ENSG00000167081 E022 75.0908012 0.0027826418 4.616722e-02 1.023455e-01 9 125935472 125935607 136 + 1.924 1.831 -0.312
ENSG00000167081 E023 1.9541144 0.0628095877 4.190296e-01 5.622845e-01 9 125949378 125949440 63 + 0.547 0.393 -0.777
ENSG00000167081 E024 64.6830344 0.0003785336 4.247427e-01 5.676830e-01 9 125960684 125960849 166 + 1.833 1.801 -0.108
ENSG00000167081 E025 27.0834624 0.0007612013 8.378302e-01 8.986056e-01 9 125962102 125962214 113 + 1.436 1.452 0.053
ENSG00000167081 E026 52.4719164 0.0006484830 6.885177e-01 7.906268e-01 9 125963012 125963101 90 + 1.738 1.721 -0.057
ENSG00000167081 E027 398.2042038 0.0003450926 2.752054e-14 8.480291e-13 9 125965831 125967377 1547 + 2.524 2.640 0.385