ENSG00000166855

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000300107 ENSG00000166855 HEK293_OSMI2_2hA HEK293_TMG_2hB CLPX protein_coding protein_coding 14.14454 5.208895 20.09814 0.1969175 0.5122238 1.945964 2.678300 2.1237591 3.636304 0.2277354 0.1284132 0.7730374 0.2481500 0.41116667 0.1809000 -0.2302667 0.0005565261 0.0005565261 FALSE TRUE
ENST00000558958 ENSG00000166855 HEK293_OSMI2_2hA HEK293_TMG_2hB CLPX protein_coding retained_intron 14.14454 5.208895 20.09814 0.1969175 0.5122238 1.945964 2.361831 0.3705509 3.912802 0.2255996 0.2784720 3.3657235 0.1250833 0.06813333 0.1955000 0.1273667 0.3149511900 0.0005565261 FALSE TRUE
MSTRG.11037.2 ENSG00000166855 HEK293_OSMI2_2hA HEK293_TMG_2hB CLPX protein_coding   14.14454 5.208895 20.09814 0.1969175 0.5122238 1.945964 7.307851 2.6157625 10.083584 0.1401260 0.7955681 1.9426302 0.5183792 0.50246667 0.5005667 -0.0019000 1.0000000000 0.0005565261 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000166855 E001 137.3825592 0.0095294913 1.870767e-06 1.464933e-05 15 65148219 65150513 2295 - 2.007 2.279 0.911
ENSG00000166855 E002 112.8527120 0.0030137300 1.436505e-03 5.428066e-03 15 65150514 65150913 400 - 1.974 2.123 0.497
ENSG00000166855 E003 101.4744597 0.0003020489 3.798773e-02 8.732860e-02 15 65152430 65152536 107 - 1.947 2.039 0.308
ENSG00000166855 E004 94.1331292 0.0002973177 7.956086e-02 1.588445e-01 15 65153547 65153639 93 - 1.919 2.001 0.277
ENSG00000166855 E005 0.7437457 0.0153787590 6.871323e-01 7.895385e-01 15 65154679 65154781 103 - 0.255 0.185 -0.588
ENSG00000166855 E006 160.3472014 0.0002444174 2.677154e-02 6.551343e-02 15 65154782 65155081 300 - 2.149 2.229 0.266
ENSG00000166855 E007 117.6727618 0.0002869875 1.972663e-02 5.090793e-02 15 65155692 65155856 165 - 2.061 1.994 -0.223
ENSG00000166855 E008 1.7090385 0.0089921357 1.243114e-01 2.261463e-01 15 65156686 65156777 92 - 0.476 0.185 -1.908
ENSG00000166855 E009 3.8429964 0.0293603485 4.781731e-01 6.166255e-01 15 65156778 65156843 66 - 0.680 0.565 -0.503
ENSG00000166855 E010 81.7334500 0.0020867232 1.098738e-01 2.053618e-01 15 65156844 65156932 89 - 1.900 1.840 -0.201
ENSG00000166855 E011 109.5254945 0.0167896206 4.806310e-01 6.188757e-01 15 65157746 65157910 165 - 2.017 1.987 -0.101
ENSG00000166855 E012 4.9041455 0.0075190986 1.127894e-02 3.182829e-02 15 65158191 65158574 384 - 0.831 0.414 -1.858
ENSG00000166855 E013 67.6516229 0.0004584126 4.752324e-01 6.139659e-01 15 65158575 65158686 112 - 1.787 1.835 0.164
ENSG00000166855 E014 48.4824482 0.0005179592 7.975326e-01 8.708115e-01 15 65158687 65158751 65 - 1.654 1.684 0.101
ENSG00000166855 E015 42.2427523 0.0005999332 4.750438e-01 6.138073e-01 15 65162604 65162645 42 - 1.614 1.588 -0.088
ENSG00000166855 E016 0.0000000       15 65162646 65162647 2 -      
ENSG00000166855 E017 68.5136921 0.0004182234 1.503228e-01 2.621151e-01 15 65164029 65164188 160 - 1.825 1.774 -0.172
ENSG00000166855 E018 34.8829127 0.0006070593 1.283337e-01 2.318274e-01 15 65166631 65166650 20 - 1.548 1.465 -0.285
ENSG00000166855 E019 77.3798268 0.0011556230 1.224258e-02 3.411567e-02 15 65166651 65166785 135 - 1.892 1.793 -0.335
ENSG00000166855 E020 80.8144067 0.0008594246 3.246725e-05 1.915190e-04 15 65178934 65179051 118 - 1.928 1.754 -0.587
ENSG00000166855 E021 81.2313631 0.0004919709 3.484238e-07 3.180488e-06 15 65180044 65180204 161 - 1.937 1.720 -0.731
ENSG00000166855 E022 0.1451727 0.0427239364 1.000000e+00   15 65184366 65184484 119 - 0.079 0.001 -6.836
ENSG00000166855 E023 64.9632752 0.0004463015 1.620594e-03 6.026906e-03 15 65185075 65185342 268 - 1.823 1.684 -0.472