ENSG00000166821

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000300056 ENSG00000166821 HEK293_OSMI2_2hA HEK293_TMG_2hB PEX11A protein_coding protein_coding 12.81266 10.44448 13.86042 1.273301 0.6366688 0.4078903 5.4101334 3.8331363 6.516722 0.6728198 0.2705434 0.76407448 0.41151667 0.36276667 0.47353333 0.11076667 0.2060031 0.0425206 FALSE TRUE
ENST00000559170 ENSG00000166821 HEK293_OSMI2_2hA HEK293_TMG_2hB PEX11A protein_coding protein_coding 12.81266 10.44448 13.86042 1.273301 0.6366688 0.4078903 2.4968020 2.2125009 2.368535 0.2087309 0.3845121 0.09788951 0.20280000 0.21786667 0.17246667 -0.04540000 0.6811111 0.0425206 FALSE FALSE
ENST00000561257 ENSG00000166821 HEK293_OSMI2_2hA HEK293_TMG_2hB PEX11A protein_coding protein_coding 12.81266 10.44448 13.86042 1.273301 0.6366688 0.4078903 3.3932694 3.6093737 2.849523 0.6750587 0.2518672 -0.33996536 0.28094583 0.34013333 0.20803333 -0.13210000 0.0425206 0.0425206 FALSE TRUE
MSTRG.11494.6 ENSG00000166821 HEK293_OSMI2_2hA HEK293_TMG_2hB PEX11A protein_coding   12.81266 10.44448 13.86042 1.273301 0.6366688 0.4078903 0.9751578 0.2871966 1.173257 0.1438085 1.1732566 1.99327329 0.06382083 0.02856667 0.07753333 0.04896667 0.6882481 0.0425206   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000166821 E001 0.1515154 0.0443201333 5.734067e-01   15 89677764 89677932 169 - 0.117 0.000 -8.076
ENSG00000166821 E002 0.8470867 0.0148275751 7.713369e-01 0.8523831612 15 89681270 89681304 35 - 0.285 0.227 -0.437
ENSG00000166821 E003 4.0483315 0.0048552985 2.936877e-01 0.4347383197 15 89681305 89681534 230 - 0.774 0.611 -0.683
ENSG00000166821 E004 1.6220536 0.0614237229 3.489177e-01 0.4931247954 15 89681535 89681540 6 - 0.500 0.308 -1.068
ENSG00000166821 E005 182.3257127 0.0046114328 1.345835e-03 0.0051292428 15 89681541 89683079 1539 - 2.330 2.201 -0.430
ENSG00000166821 E006 90.2135476 0.0023116973 2.991523e-01 0.4406479712 15 89683080 89683250 171 - 1.996 1.941 -0.184
ENSG00000166821 E007 134.0953503 0.0003024063 8.264577e-04 0.0033541118 15 89683251 89683502 252 - 2.094 2.175 0.272
ENSG00000166821 E008 69.0353015 0.0004100943 3.373292e-04 0.0015296201 15 89683503 89683553 51 - 1.779 1.912 0.450
ENSG00000166821 E009 137.2646228 0.0002502577 7.601128e-05 0.0004091172 15 89683554 89683855 302 - 2.095 2.192 0.324
ENSG00000166821 E010 64.2631776 0.0019760527 9.882557e-01 0.9967316254 15 89683856 89683948 93 - 1.827 1.812 -0.052
ENSG00000166821 E011 77.3353920 0.0053551319 2.053824e-01 0.3328948187 15 89686431 89686546 116 - 1.939 1.857 -0.277
ENSG00000166821 E012 79.0231047 0.0004043230 8.722534e-03 0.0255928112 15 89690577 89690783 207 - 1.965 1.852 -0.380