ENSG00000166681

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000361298 ENSG00000166681 HEK293_OSMI2_2hA HEK293_TMG_2hB BEX3 protein_coding protein_coding 496.5742 962.4176 254.3548 141.7635 2.503046 -1.919779 376.84531 742.5123 181.09972 113.53082 2.2708000 -2.035570 0.7425208 0.7700000 0.7119667 -0.05803333 0.0006355388 2.92365e-05 FALSE  
ENST00000372645 ENSG00000166681 HEK293_OSMI2_2hA HEK293_TMG_2hB BEX3 protein_coding protein_coding 496.5742 962.4176 254.3548 141.7635 2.503046 -1.919779 92.70522 168.5644 61.52853 20.50205 0.4366806 -1.453823 0.2020667 0.1767667 0.2419000 0.06513333 0.0000292365 2.92365e-05 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000166681 E001 21.73616 0.0245689130 2.551111e-02 0.0629338367 X 103376207 103376394 188 + 1.422 1.189 -0.815
ENSG00000166681 E002 18.98035 0.0010159943 7.906147e-03 0.0235424758 X 103376395 103376406 12 + 1.352 1.146 -0.726
ENSG00000166681 E003 619.22782 0.0008060106 2.173971e-02 0.0551371166 X 103376407 103376537 131 + 2.696 2.671 -0.083
ENSG00000166681 E004 3222.51726 0.0008551996 4.230351e-01 0.5661596355 X 103376538 103376613 76 + 3.360 3.393 0.110
ENSG00000166681 E005 18.26664 0.0011502504 9.589895e-01 0.9782922704 X 103376916 103377027 112 + 1.147 1.184 0.134
ENSG00000166681 E006 3932.89773 0.0004921073 6.269972e-02 0.1312002200 X 103377028 103377106 79 + 3.472 3.475 0.010
ENSG00000166681 E007 32.14581 0.0006477717 2.270769e-01 0.3589060299 X 103377107 103377261 155 + 1.463 1.407 -0.193
ENSG00000166681 E008 664.77600 0.0032852804 1.929876e-04 0.0009338914 X 103377463 103377474 12 + 2.783 2.684 -0.329
ENSG00000166681 E009 782.48724 0.0022072742 8.210155e-05 0.0004379404 X 103377475 103377509 35 + 2.841 2.759 -0.275
ENSG00000166681 E010 11104.16608 0.0015664878 1.172258e-02 0.0328716227 X 103377510 103378164 655 + 3.883 3.931 0.157