ENSG00000166483

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000450114 ENSG00000166483 HEK293_OSMI2_2hA HEK293_TMG_2hB WEE1 protein_coding protein_coding 25.10768 18.56103 32.43995 2.69074 1.499196 0.8051622 9.762746 4.212365 18.209575 0.6977190 0.8200864 2.1093661 0.3821542 0.23636667 0.563400000 0.32703333 5.010172e-04 9.644661e-49 FALSE TRUE
ENST00000530175 ENSG00000166483 HEK293_OSMI2_2hA HEK293_TMG_2hB WEE1 protein_coding nonsense_mediated_decay 25.10768 18.56103 32.43995 2.69074 1.499196 0.8051622 1.384760 3.962577 0.263430 2.6269697 0.1897723 -3.8608319 0.0662000 0.19216667 0.008733333 -0.18343333 4.562775e-01 9.644661e-49 TRUE TRUE
ENST00000530712 ENSG00000166483 HEK293_OSMI2_2hA HEK293_TMG_2hB WEE1 protein_coding protein_coding 25.10768 18.56103 32.43995 2.69074 1.499196 0.8051622 2.761863 1.578559 2.173431 0.4442764 0.6350572 0.4588782 0.1165042 0.08736667 0.066600000 -0.02076667 8.084416e-01 9.644661e-49 FALSE TRUE
ENST00000532275 ENSG00000166483 HEK293_OSMI2_2hA HEK293_TMG_2hB WEE1 protein_coding retained_intron 25.10768 18.56103 32.43995 2.69074 1.499196 0.8051622 4.255510 1.116055 7.220020 0.6046208 1.1271238 2.6827218 0.1561292 0.05813333 0.221166667 0.16303333 2.705378e-01 9.644661e-49 FALSE FALSE
ENST00000681684 ENSG00000166483 HEK293_OSMI2_2hA HEK293_TMG_2hB WEE1 protein_coding protein_coding 25.10768 18.56103 32.43995 2.69074 1.499196 0.8051622 4.065468 7.176240 0.000000 0.4469862 0.0000000 -9.4890933 0.1799667 0.39606667 0.000000000 -0.39606667 9.644661e-49 9.644661e-49 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000166483 E001 148.452339 0.0108060858 3.008665e-14 9.221527e-13 11 9573670 9574509 840 + 2.306 1.878 -1.436
ENSG00000166483 E002 3.407240 0.1561833321 2.045846e-02 5.245569e-02 11 9574687 9575375 689 + 0.787 0.266 -2.601
ENSG00000166483 E003 0.294498 0.3768037877 4.435221e-01   11 9575490 9575495 6 + 0.179 0.000 -9.100
ENSG00000166483 E004 1.210371 0.4676863815 3.573895e-01 5.017962e-01 11 9575496 9575887 392 + 0.445 0.151 -2.103
ENSG00000166483 E005 188.979435 0.0003231399 5.430372e-06 3.834536e-05 11 9575888 9576093 206 + 2.311 2.226 -0.281
ENSG00000166483 E006 156.528195 0.0017389081 1.223304e-02 3.409462e-02 11 9576230 9576293 64 + 2.217 2.166 -0.170
ENSG00000166483 E007 206.536799 0.0011649952 2.227436e-01 3.536490e-01 11 9576487 9576659 173 + 2.314 2.313 -0.003
ENSG00000166483 E008 135.922953 0.0013974506 3.600513e-01 5.043636e-01 11 9577142 9577263 122 + 2.103 2.169 0.220
ENSG00000166483 E009 19.466773 0.0009600435 3.069389e-09 4.155554e-08 11 9577264 9577784 521 + 1.453 0.967 -1.727
ENSG00000166483 E010 7.545087 0.0341250550 2.205080e-03 7.858421e-03 11 9577785 9577796 12 + 1.069 0.633 -1.704
ENSG00000166483 E011 10.925950 0.0372371882 5.727384e-04 2.431725e-03 11 9577797 9577895 99 + 1.220 0.744 -1.777
ENSG00000166483 E012 9.562146 0.0036175184 1.065540e-09 1.571900e-08 11 9577896 9578027 132 + 1.214 0.424 -3.215
ENSG00000166483 E013 89.071531 0.0083428897 8.869986e-24 8.573430e-22 11 9578028 9581226 3199 + 2.123 1.507 -2.080
ENSG00000166483 E014 17.045652 0.0011306060 5.489760e-06 3.871421e-05 11 9581227 9581531 305 + 1.376 1.004 -1.324
ENSG00000166483 E015 82.556381 0.0003390947 8.561032e-01 9.110508e-01 11 9581532 9581543 12 + 1.901 1.946 0.151
ENSG00000166483 E016 183.605623 0.0012971990 6.984128e-02 1.431145e-01 11 9581544 9581678 135 + 2.222 2.312 0.301
ENSG00000166483 E017 3.292181 0.0098945655 2.073868e-01 3.353718e-01 11 9585183 9585257 75 + 0.707 0.541 -0.727
ENSG00000166483 E018 175.075374 0.0004022446 9.601702e-01 9.789274e-01 11 9585258 9585353 96 + 2.227 2.266 0.130
ENSG00000166483 E019 187.528063 0.0007824978 5.500208e-01 6.796983e-01 11 9585442 9585527 86 + 2.266 2.285 0.065
ENSG00000166483 E020 141.088814 0.0017005204 9.548201e-01 9.756759e-01 11 9586449 9586497 49 + 2.136 2.169 0.108
ENSG00000166483 E021 199.817894 0.0001990705 4.038540e-01 5.476267e-01 11 9586498 9586619 122 + 2.295 2.312 0.057
ENSG00000166483 E022 96.688960 0.0003073281 9.597620e-01 9.787039e-01 11 9586711 9586713 3 + 1.974 2.010 0.120
ENSG00000166483 E023 208.341369 0.0001968689 4.481672e-01 5.894837e-01 11 9586714 9586856 143 + 2.295 2.351 0.188
ENSG00000166483 E024 189.239796 0.0003227685 3.966867e-01 5.404572e-01 11 9588449 9588537 89 + 2.253 2.313 0.201
ENSG00000166483 E025 780.882627 0.0068861431 1.470249e-09 2.111708e-08 11 9588538 9589985 1448 + 2.770 3.011 0.800
ENSG00000166483 E026 2.637606 0.0722401818 7.716979e-01 8.526226e-01 11 9593225 9593457 233 + 0.520 0.592 0.332