ENSG00000166479

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000299608 ENSG00000166479 HEK293_OSMI2_2hA HEK293_TMG_2hB TMX3 protein_coding protein_coding 12.41228 2.445878 19.43243 0.3757485 0.5852414 2.984898 9.1425128 1.9129953 14.3820288 0.2690490 0.477201310 2.9038429 0.7392667 0.7857667 0.74086667 -0.04490000 6.822315e-01 1.782757e-07 FALSE TRUE
ENST00000562706 ENSG00000166479 HEK293_OSMI2_2hA HEK293_TMG_2hB TMX3 protein_coding protein_coding 12.41228 2.445878 19.43243 0.3757485 0.5852414 2.984898 0.2631146 0.2668522 0.2107722 0.1011264 0.008257142 -0.3265575 0.0560750 0.1027333 0.01086667 -0.09186667 1.782757e-07 1.782757e-07 FALSE TRUE
ENST00000564631 ENSG00000166479 HEK293_OSMI2_2hA HEK293_TMG_2hB TMX3 protein_coding nonsense_mediated_decay 12.41228 2.445878 19.43243 0.3757485 0.5852414 2.984898 0.7605307 0.0000000 1.4093324 0.0000000 0.308022426 7.1490686 0.0369500 0.0000000 0.07246667 0.07246667 2.527622e-04 1.782757e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000166479 E001 596.2235221 0.0047556715 5.188795e-20 3.298663e-18 18 68673688 68676709 3022 - 2.532 2.831 0.994
ENSG00000166479 E002 50.0533826 0.0005030902 6.065746e-02 1.277344e-01 18 68676710 68676838 129 - 1.523 1.449 -0.252
ENSG00000166479 E003 70.2372819 0.0019691295 6.411467e-04 2.684569e-03 18 68676839 68677027 189 - 1.674 1.494 -0.616
ENSG00000166479 E004 76.0150480 0.0004960210 3.900388e-05 2.254099e-04 18 68677028 68677193 166 - 1.710 1.494 -0.739
ENSG00000166479 E005 50.9717730 0.0004897072 3.452020e-03 1.158749e-02 18 68679463 68679531 69 - 1.535 1.364 -0.589
ENSG00000166479 E006 0.0000000       18 68679532 68679577 46 -      
ENSG00000166479 E007 74.8547276 0.0003791447 1.945818e-08 2.274788e-07 18 68680981 68681110 130 - 1.710 1.345 -1.253
ENSG00000166479 E008 0.8878743 0.0131352372 6.003007e-01 7.215477e-01 18 68681293 68681322 30 - 0.213 0.000 -12.246
ENSG00000166479 E009 55.9905016 0.0007898526 3.847944e-09 5.118174e-08 18 68682925 68682981 57 - 1.594 1.082 -1.789
ENSG00000166479 E010 51.8948203 0.0959829047 1.674210e-03 6.200376e-03 18 68684190 68684243 54 - 1.562 1.059 -1.759
ENSG00000166479 E011 48.5401306 0.0004960030 2.964542e-09 4.025975e-08 18 68684428 68684485 58 - 1.536 0.956 -2.053
ENSG00000166479 E012 54.8747347 0.1493305538 9.923702e-03 2.857340e-02 18 68687667 68687765 99 - 1.586 1.096 -1.710
ENSG00000166479 E013 1.9261181 0.0075688721 6.915426e-01 7.929996e-01 18 68687766 68687977 212 - 0.355 0.302 -0.330
ENSG00000166479 E014 11.0548055 0.0090230495 7.188023e-01 8.137913e-01 18 68688417 68689054 638 - 0.883 1.003 0.449
ENSG00000166479 E015 2.9623873 0.0050714891 6.011280e-02 1.267947e-01 18 68689687 68689981 295 - 0.492 0.000 -13.949
ENSG00000166479 E016 3.2150511 0.0173800773 2.567107e-01 3.936785e-01 18 68691014 68691294 281 - 0.429 0.702 1.261
ENSG00000166479 E017 51.3737238 0.0181249867 1.078212e-03 4.228806e-03 18 68691295 68691361 67 - 1.549 1.236 -1.084
ENSG00000166479 E018 3.1772070 0.0050892927 2.112278e-07 2.015740e-06 18 68696936 68697225 290 - 0.265 1.116 3.838
ENSG00000166479 E019 47.6947105 0.0004389542 6.825823e-01 7.860123e-01 18 68697226 68697303 78 - 1.489 1.534 0.155
ENSG00000166479 E020 1.2189160 0.0108560246 5.463691e-02 1.173653e-01 18 68697376 68697930 555 - 0.184 0.604 2.517
ENSG00000166479 E021 0.0000000       18 68697931 68697931 1 -      
ENSG00000166479 E022 65.3792562 0.0004301666 9.750679e-01 9.884615e-01 18 68697932 68698031 100 - 1.617 1.693 0.255
ENSG00000166479 E023 66.6352040 0.0003852209 6.215878e-01 7.385351e-01 18 68700405 68700485 81 - 1.629 1.674 0.153
ENSG00000166479 E024 3.4052923 0.0045143877 2.475413e-01 3.830555e-01 18 68701616 68701667 52 - 0.521 0.302 -1.204
ENSG00000166479 E025 50.6823574 0.0007484450 4.423456e-01 5.839713e-01 18 68701745 68701790 46 - 1.516 1.534 0.061
ENSG00000166479 E026 0.5975289 0.0268153124 1.000000e+00 1.000000e+00 18 68706280 68706408 129 - 0.153 0.000 -11.706
ENSG00000166479 E027 64.7393890 0.0019720357 8.847521e-01 9.303289e-01 18 68710021 68710144 124 - 1.615 1.683 0.231
ENSG00000166479 E028 37.1839467 0.0039129184 9.529719e-01 9.745623e-01 18 68711364 68711403 40 - 1.381 1.464 0.286
ENSG00000166479 E029 33.7121363 0.0006089774 6.757018e-01 7.808896e-01 18 68713846 68713900 55 - 1.337 1.449 0.388
ENSG00000166479 E030 0.4375944 0.0293218571 1.000000e+00 1.000000e+00 18 68714263 68714443 181 - 0.119 0.000 -11.299
ENSG00000166479 E031 35.2275882 0.0016585873 8.379801e-01 8.987060e-01 18 68714936 68715108 173 - 1.356 1.449 0.323