ENSG00000166441

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000314138 ENSG00000166441 HEK293_OSMI2_2hA HEK293_TMG_2hB RPL27A protein_coding protein_coding 1611.064 2960.615 1131.64 114.1599 2.406431 -1.387474 107.1319 128.5051 123.71334 12.29498 7.877299 -0.05482063 0.07578750 0.04333333 0.10936667 0.066033333 9.805428e-10 1.436791e-11 FALSE  
ENST00000531978 ENSG00000166441 HEK293_OSMI2_2hA HEK293_TMG_2hB RPL27A protein_coding protein_coding 1611.064 2960.615 1131.64 114.1599 2.406431 -1.387474 1308.4163 2418.9311 918.32950 82.90448 5.659438 -1.39727616 0.81105000 0.81733333 0.81150000 -0.005833333 8.287290e-01 1.436791e-11 FALSE  
MSTRG.5123.1 ENSG00000166441 HEK293_OSMI2_2hA HEK293_TMG_2hB RPL27A protein_coding   1611.064 2960.615 1131.64 114.1599 2.406431 -1.387474 111.2734 278.1788 35.69832 30.69845 3.736602 -2.96173219 0.05783333 0.09343333 0.03153333 -0.061900000 3.813222e-09 1.436791e-11    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000166441 E001 4.107272e+02 5.025327e-03 1.535264e-02 4.129063e-02 11 8682727 8682787 61 + 2.447 2.556 0.365
ENSG00000166441 E002 4.338039e+02 5.822734e-03 2.423270e-02 6.029118e-02 11 8682788 8682788 1 + 2.472 2.579 0.355
ENSG00000166441 E003 4.447538e+02 5.448724e-03 3.015766e-02 7.227697e-02 11 8682789 8682791 3 + 2.488 2.589 0.334
ENSG00000166441 E004 2.904360e+03 2.602836e-03 3.075724e-09 4.163238e-08 11 8682792 8682809 18 + 3.492 3.346 -0.487
ENSG00000166441 E005 6.741450e+03 2.431290e-04 4.927185e-119 2.456422e-115 11 8682810 8682816 7 + 3.891 3.699 -0.641
ENSG00000166441 E006 1.190975e+02 4.086932e-02 4.812161e-01 6.194507e-01 11 8682817 8683041 225 + 1.936 2.015 0.265
ENSG00000166441 E007 2.027317e+02 1.563143e-02 6.472003e-01 7.589235e-01 11 8683042 8683200 159 + 2.198 2.236 0.129
ENSG00000166441 E008 2.294684e+02 1.137409e-03 6.073962e-02 1.278733e-01 11 8683201 8683201 1 + 2.226 2.299 0.243
ENSG00000166441 E009 1.273251e+04 6.649238e-04 6.028202e-05 3.328354e-04 11 8683202 8683214 13 + 4.057 4.015 -0.138
ENSG00000166441 E010 2.177967e+04 3.227658e-04 4.975059e-01 6.340540e-01 11 8683215 8683265 51 + 4.246 4.261 0.049
ENSG00000166441 E011 2.025079e+02 1.828776e-03 1.576360e-08 1.872330e-07 11 8683266 8683779 514 + 2.365 2.184 -0.604
ENSG00000166441 E012 3.766458e+01 6.776334e-04 8.372539e-02 1.653775e-01 11 8683863 8683960 98 + 1.587 1.491 -0.328
ENSG00000166441 E013 6.981494e+01 1.292744e-02 4.116416e-01 5.551824e-01 11 8683961 8684005 45 + 1.716 1.789 0.248
ENSG00000166441 E014 3.304686e+04 3.890097e-04 1.392804e-01 2.470692e-01 11 8684006 8684081 76 + 4.422 4.444 0.071
ENSG00000166441 E015 2.374909e+01 5.127275e-03 5.547838e-21 3.993242e-19 11 8684082 8684226 145 + 1.747 1.038 -2.467
ENSG00000166441 E016 1.982839e+01 9.303361e-04 2.843267e-11 5.498096e-10 11 8684227 8684258 32 + 1.575 1.075 -1.747
ENSG00000166441 E017 8.035851e+01 7.460178e-03 6.967701e-13 1.754166e-11 11 8684259 8684685 427 + 2.108 1.708 -1.347
ENSG00000166441 E018 2.671491e+01 5.705380e-03 3.176959e-06 2.363222e-05 11 8684686 8684717 32 + 1.611 1.271 -1.173
ENSG00000166441 E019 3.909219e+04 6.362299e-05 7.056791e-12 1.507129e-10 11 8684718 8684822 105 + 4.484 4.520 0.119
ENSG00000166441 E020 3.694520e+04 2.020691e-04 1.483294e-07 1.457498e-06 11 8684823 8684892 70 + 4.454 4.497 0.141
ENSG00000166441 E021 1.929244e+02 7.812699e-04 1.856331e-07 1.789611e-06 11 8684893 8685415 523 + 2.321 2.172 -0.497
ENSG00000166441 E022 3.626774e+04 9.830589e-04 7.179668e-01 8.132215e-01 11 8685678 8689129 3452 + 4.467 4.483 0.051
ENSG00000166441 E023 1.531493e+02 1.262872e-02 6.113249e-08 6.472930e-07 11 8689130 8689303 174 + 2.336 2.019 -1.061
ENSG00000166441 E024 1.754359e+02 2.276379e-02 7.223852e-06 4.957390e-05 11 8689304 8689872 569 + 2.400 2.072 -1.094
ENSG00000166441 E025 1.515154e-01 4.324333e-02 1.569321e-01   11 8714684 8714759 76 + 0.191 0.000 -9.744