ENSG00000166411

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000558535 ENSG00000166411 HEK293_OSMI2_2hA HEK293_TMG_2hB IDH3A protein_coding processed_transcript 52.84207 30.73341 81.91433 2.88056 2.27988 1.414015 8.291209 8.4183637 7.315859 1.3657712 2.0133442 -0.2022547 0.18270000 0.2706333 0.0907000 -0.17993333 0.01025216 6.712784e-07 FALSE TRUE
ENST00000558554 ENSG00000166411 HEK293_OSMI2_2hA HEK293_TMG_2hB IDH3A protein_coding protein_coding 52.84207 30.73341 81.91433 2.88056 2.27988 1.414015 13.408223 9.0367604 20.841446 0.2515027 1.6410353 1.2046743 0.26176250 0.2976333 0.2540000 -0.04363333 0.50552947 6.712784e-07 FALSE TRUE
ENST00000558933 ENSG00000166411 HEK293_OSMI2_2hA HEK293_TMG_2hB IDH3A protein_coding nonsense_mediated_decay 52.84207 30.73341 81.91433 2.88056 2.27988 1.414015 6.051210 0.8301617 10.809176 0.4713541 1.4252612 3.6867798 0.09393333 0.0270000 0.1312333 0.10423333 0.18146151 6.712784e-07 FALSE FALSE
ENST00000559881 ENSG00000166411 HEK293_OSMI2_2hA HEK293_TMG_2hB IDH3A protein_coding protein_coding 52.84207 30.73341 81.91433 2.88056 2.27988 1.414015 3.559094 0.9655861 5.223356 0.3278532 0.5714169 2.4233953 0.06397500 0.0336000 0.0638000 0.03020000 0.32307747 6.712784e-07 FALSE FALSE
ENST00000560396 ENSG00000166411 HEK293_OSMI2_2hA HEK293_TMG_2hB IDH3A protein_coding nonsense_mediated_decay 52.84207 30.73341 81.91433 2.88056 2.27988 1.414015 11.535255 7.5650274 16.491113 0.2757217 1.9982091 1.1232403 0.21175417 0.2486333 0.2023667 -0.04626667 0.53273377 6.712784e-07 FALSE FALSE
ENST00000561279 ENSG00000166411 HEK293_OSMI2_2hA HEK293_TMG_2hB IDH3A protein_coding protein_coding 52.84207 30.73341 81.91433 2.88056 2.27988 1.414015 1.768685 0.0000000 6.418908 0.0000000 3.2507956 9.3284299 0.02145000 0.0000000 0.0776000 0.07760000 0.25604907 6.712784e-07 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000166411 E001 0.0000000       15 78131498 78131631 134 +      
ENSG00000166411 E002 0.0000000       15 78146316 78146590 275 +      
ENSG00000166411 E003 1.5156041 0.0087599032 8.374299e-01 8.983701e-01 15 78149326 78149359 34 + 0.401 0.391 -0.055
ENSG00000166411 E004 1.6607768 0.0087058119 6.824864e-01 7.859210e-01 15 78149360 78149361 2 + 0.437 0.391 -0.248
ENSG00000166411 E005 1.9638077 0.0111458459 4.393559e-01 5.811055e-01 15 78149362 78149367 6 + 0.501 0.391 -0.570
ENSG00000166411 E006 4.5372234 0.0034559659 1.177264e-01 2.166909e-01 15 78149368 78149372 5 + 0.793 0.594 -0.832
ENSG00000166411 E007 11.1091982 0.0015321653 5.392012e-01 6.704827e-01 15 78149373 78149376 4 + 1.074 1.051 -0.083
ENSG00000166411 E008 13.8156057 0.0012264365 4.443136e-01 5.858957e-01 15 78149377 78149377 1 + 1.167 1.136 -0.109
ENSG00000166411 E009 15.0323316 0.0011561243 5.117346e-01 6.466899e-01 15 78149378 78149379 2 + 1.198 1.180 -0.062
ENSG00000166411 E010 41.7784403 0.0005030158 1.192805e-05 7.786543e-05 15 78149380 78149391 12 + 1.680 1.459 -0.754
ENSG00000166411 E011 82.6473239 0.0002991568 8.919476e-03 2.608029e-02 15 78149392 78149403 12 + 1.926 1.862 -0.217
ENSG00000166411 E012 94.8173509 0.0002944873 3.154154e-02 7.496205e-02 15 78149404 78149408 5 + 1.976 1.936 -0.132
ENSG00000166411 E013 121.1643049 0.0002601994 7.085877e-04 2.929913e-03 15 78149409 78149430 22 + 2.092 2.019 -0.246
ENSG00000166411 E014 3.1784307 0.0094423435 4.527238e-01 5.935485e-01 15 78150804 78150960 157 + 0.629 0.537 -0.415
ENSG00000166411 E015 0.7406253 0.0153941857 9.488481e-02 1.827090e-01 15 78151289 78151544 256 + 0.319 0.001 -9.818
ENSG00000166411 E016 4.4895415 0.0203994700 3.403224e-01 4.842900e-01 15 78154435 78154819 385 + 0.762 0.645 -0.487
ENSG00000166411 E017 154.4697883 0.0003229433 1.820783e-05 1.140228e-04 15 78155213 78155251 39 + 2.202 2.112 -0.303
ENSG00000166411 E018 140.0928209 0.0051060464 5.512594e-03 1.731943e-02 15 78155252 78155275 24 + 2.161 2.064 -0.324
ENSG00000166411 E019 8.0802088 0.0100849076 1.497194e-01 2.612956e-01 15 78155276 78155732 457 + 0.986 0.836 -0.568
ENSG00000166411 E020 8.5708147 0.0033237491 9.904321e-05 5.173552e-04 15 78156908 78156922 15 + 1.082 0.594 -1.920
ENSG00000166411 E021 14.5286926 0.0032472768 1.863874e-04 9.052823e-04 15 78156923 78157030 108 + 1.269 0.920 -1.266
ENSG00000166411 E022 15.4495216 0.0012337788 6.006136e-03 1.862632e-02 15 78157031 78157162 132 + 1.269 1.051 -0.780
ENSG00000166411 E023 7.0717811 0.0024127952 2.111558e-03 7.570411e-03 15 78157163 78157547 385 + 0.986 0.594 -1.568
ENSG00000166411 E024 151.8783889 0.0004674362 7.626685e-05 4.103762e-04 15 78157548 78157572 25 + 2.193 2.110 -0.279
ENSG00000166411 E025 205.7511495 0.0005259603 3.290514e-02 7.761373e-02 15 78157573 78157631 59 + 2.302 2.285 -0.056
ENSG00000166411 E026 0.6934950 0.1486196935 4.794615e-01 6.178416e-01 15 78159970 78160091 122 + 0.156 0.298 1.188
ENSG00000166411 E027 275.9824065 0.0001864406 5.642663e-01 6.915778e-01 15 78160092 78160206 115 + 2.413 2.444 0.103
ENSG00000166411 E028 278.5593840 0.0021965966 8.427999e-02 1.662684e-01 15 78161581 78161685 105 + 2.433 2.420 -0.044
ENSG00000166411 E029 303.9661633 0.0020864035 2.368726e-03 8.364049e-03 15 78161686 78161768 83 + 2.483 2.433 -0.167
ENSG00000166411 E030 0.3332198 0.0330164004 5.997204e-01   15 78162209 78162233 25 + 0.085 0.173 1.169
ENSG00000166411 E031 297.8339426 0.0004478742 1.894137e-06 1.481458e-05 15 78162234 78162367 134 + 2.481 2.413 -0.227
ENSG00000166411 E032 5.3970050 0.0031083508 5.289610e-01 6.618106e-01 15 78163470 78163506 37 + 0.745 0.866 0.476
ENSG00000166411 E033 260.2833103 0.0001798397 2.379756e-01 3.717850e-01 15 78163507 78163609 103 + 2.393 2.410 0.055
ENSG00000166411 E034 224.8855691 0.0002242793 6.096172e-01 7.290896e-01 15 78163716 78163780 65 + 2.325 2.356 0.104
ENSG00000166411 E035 291.4813163 0.0001549665 2.820462e-02 6.842026e-02 15 78164992 78165076 85 + 2.450 2.447 -0.011
ENSG00000166411 E036 2.9194573 0.1779661664 2.539628e-01 3.905837e-01 15 78166105 78166149 45 + 0.471 0.730 1.156
ENSG00000166411 E037 314.0182985 0.0001515565 4.401173e-03 1.426793e-02 15 78166150 78166240 91 + 2.487 2.471 -0.052
ENSG00000166411 E038 287.4113176 0.0001835889 6.796427e-02 1.399844e-01 15 78166241 78166302 62 + 2.442 2.446 0.014
ENSG00000166411 E039 0.7543549 0.5560906985 8.476498e-01 9.053963e-01 15 78166303 78166473 171 + 0.274 0.169 -0.886
ENSG00000166411 E040 432.2377723 0.0011322658 5.890207e-01 7.121473e-01 15 78168922 78169077 156 + 2.599 2.652 0.179
ENSG00000166411 E041 1359.8674417 0.0023546444 2.589859e-18 1.356111e-16 15 78169078 78171049 1972 + 3.027 3.242 0.712
ENSG00000166411 E042 14.7700279 0.0168729747 2.443512e-02 6.072144e-02 15 78171050 78171945 896 + 1.066 1.348 1.000