ENSG00000166398

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000299505 ENSG00000166398 HEK293_OSMI2_2hA HEK293_TMG_2hB GARRE1 protein_coding protein_coding 4.261794 3.182873 5.298722 0.3006883 0.1276603 0.7335093 3.3107746 1.2493900 5.0042127 0.1780395 0.09134088 1.993298 0.72547083 0.39973333 0.94476667 0.54503333 3.558620e-11 3.55862e-11 FALSE TRUE
ENST00000588338 ENSG00000166398 HEK293_OSMI2_2hA HEK293_TMG_2hB GARRE1 protein_coding processed_transcript 4.261794 3.182873 5.298722 0.3006883 0.1276603 0.7335093 0.2114312 0.6233563 0.0000000 0.3849259 0.00000000 -5.984946 0.05801250 0.17820000 0.00000000 -0.17820000 9.953957e-02 3.55862e-11   FALSE
ENST00000588974 ENSG00000166398 HEK293_OSMI2_2hA HEK293_TMG_2hB GARRE1 protein_coding retained_intron 4.261794 3.182873 5.298722 0.3006883 0.1276603 0.7335093 0.2542005 0.3873394 0.1634268 0.1214596 0.03456233 -1.196045 0.07332917 0.12746667 0.03106667 -0.09640000 6.337579e-02 3.55862e-11 FALSE TRUE
MSTRG.16948.1 ENSG00000166398 HEK293_OSMI2_2hA HEK293_TMG_2hB GARRE1 protein_coding   4.261794 3.182873 5.298722 0.3006883 0.1276603 0.7335093 0.1933086 0.6100308 0.0000000 0.3058298 0.00000000 -5.954268 0.06229167 0.21180000 0.00000000 -0.21180000 1.507016e-01 3.55862e-11 FALSE TRUE
MSTRG.16948.5 ENSG00000166398 HEK293_OSMI2_2hA HEK293_TMG_2hB GARRE1 protein_coding   4.261794 3.182873 5.298722 0.3006883 0.1276603 0.7335093 0.1316985 0.2699585 0.0397949 0.2699585 0.03979490 -2.491143 0.03412500 0.07146667 0.00720000 -0.06426667 8.849093e-01 3.55862e-11 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000166398 E001 0.2214452 0.0420446298 2.525778e-01   19 34254552 34254553 2 + 0.000 0.174 10.312
ENSG00000166398 E002 2.2958419 0.1937556293 7.893066e-01 8.651256e-01 19 34254554 34254579 26 + 0.499 0.480 -0.093
ENSG00000166398 E003 2.2958419 0.1937556293 7.893066e-01 8.651256e-01 19 34254580 34254580 1 + 0.499 0.480 -0.093
ENSG00000166398 E004 5.5263347 0.0037445940 1.511726e-01 2.632689e-01 19 34254581 34254596 16 + 0.833 0.694 -0.560
ENSG00000166398 E005 8.3650019 0.0216925290 1.689510e-01 2.868162e-01 19 34254597 34254614 18 + 0.992 0.869 -0.463
ENSG00000166398 E006 0.0000000       19 34265558 34265567 10 +      
ENSG00000166398 E007 0.2214452 0.0420446298 2.525778e-01   19 34265568 34265621 54 + 0.000 0.174 12.596
ENSG00000166398 E008 0.5503986 0.0216653336 2.556536e-01 3.925595e-01 19 34285150 34285170 21 + 0.085 0.298 2.196
ENSG00000166398 E009 0.8438645 0.0144626106 2.446539e-01 3.796433e-01 19 34285171 34285273 103 + 0.155 0.394 1.779
ENSG00000166398 E010 26.9192722 0.0126008309 6.184645e-04 2.601324e-03 19 34299679 34299923 245 + 1.496 1.249 -0.857
ENSG00000166398 E011 32.1402178 0.0006810147 5.218364e-07 4.598797e-06 19 34299924 34300264 341 + 1.577 1.305 -0.939
ENSG00000166398 E012 46.5252848 0.0004605240 2.655738e-04 1.239552e-03 19 34300265 34300754 490 + 1.698 1.573 -0.424
ENSG00000166398 E013 40.0068210 0.0006238525 3.207756e-03 1.087801e-02 19 34300755 34300968 214 + 1.624 1.525 -0.339
ENSG00000166398 E014 26.5411704 0.0013690506 3.342973e-02 7.863863e-02 19 34319907 34319972 66 + 1.446 1.365 -0.280
ENSG00000166398 E015 32.4876762 0.0080317429 3.172931e-03 1.077553e-02 19 34319973 34320116 144 + 1.554 1.391 -0.560
ENSG00000166398 E016 27.5022298 0.0096908160 1.095951e-02 3.106620e-02 19 34327421 34327561 141 + 1.481 1.335 -0.504
ENSG00000166398 E017 21.1688199 0.0553634153 3.628500e-01 5.072638e-01 19 34327771 34327866 96 + 1.340 1.302 -0.132
ENSG00000166398 E018 29.4271027 0.0011043542 1.130351e-01 2.099675e-01 19 34327990 34328151 162 + 1.475 1.441 -0.118
ENSG00000166398 E019 36.5900071 0.0005516399 3.978388e-03 1.308201e-02 19 34330189 34330347 159 + 1.587 1.485 -0.351
ENSG00000166398 E020 30.9160945 0.0006985568 4.101862e-02 9.291501e-02 19 34333704 34333801 98 + 1.508 1.448 -0.206
ENSG00000166398 E021 32.7582427 0.0006284794 4.177193e-01 5.610429e-01 19 34339867 34339992 126 + 1.502 1.525 0.078
ENSG00000166398 E022 132.6444007 0.0047870369 4.811324e-01 6.193715e-01 19 34341422 34342455 1034 + 2.091 2.135 0.147
ENSG00000166398 E023 29.5671310 0.0008696158 1.802042e-01 3.014335e-01 19 34347877 34348042 166 + 1.472 1.456 -0.055
ENSG00000166398 E024 5.0961377 0.0038152150 6.023030e-01 7.232836e-01 19 34348462 34349015 554 + 0.707 0.840 0.532
ENSG00000166398 E025 25.0651847 0.0008986154 1.263280e-01 2.290342e-01 19 34349016 34349119 104 + 1.411 1.373 -0.129
ENSG00000166398 E026 18.8586783 0.0011713518 5.094727e-01 6.448080e-01 19 34349120 34349153 34 + 1.276 1.294 0.066
ENSG00000166398 E027 25.6766976 0.0008025878 4.964756e-01 6.331124e-01 19 34351514 34351592 79 + 1.365 1.485 0.413
ENSG00000166398 E028 302.6900119 0.0072717796 9.694533e-14 2.763051e-12 19 34352647 34355566 2920 + 2.325 2.642 1.059