ENSG00000166263

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000376352 ENSG00000166263 HEK293_OSMI2_2hA HEK293_TMG_2hB STXBP4 protein_coding protein_coding 3.743553 0.8679239 5.475703 0.2086145 0.5609381 2.643509 0.9497177 0.41587698 1.1694012 0.04273030 0.05893544 1.4695459 0.38197917 0.51626667 0.2175333 -0.298733333 0.01793348 0.01793348 FALSE TRUE
ENST00000405898 ENSG00000166263 HEK293_OSMI2_2hA HEK293_TMG_2hB STXBP4 protein_coding protein_coding 3.743553 0.8679239 5.475703 0.2086145 0.5609381 2.643509 0.1677798 0.10881388 0.1656712 0.04488870 0.08683945 0.5641744 0.07886667 0.15866667 0.0333000 -0.125366667 0.35906966 0.01793348 FALSE TRUE
ENST00000461444 ENSG00000166263 HEK293_OSMI2_2hA HEK293_TMG_2hB STXBP4 protein_coding retained_intron 3.743553 0.8679239 5.475703 0.2086145 0.5609381 2.643509 0.5142783 0.17219268 0.6731376 0.17219268 0.67313762 1.9067112 0.12099167 0.13400000 0.1028000 -0.031200000 0.81330174 0.01793348   FALSE
MSTRG.14710.1 ENSG00000166263 HEK293_OSMI2_2hA HEK293_TMG_2hB STXBP4 protein_coding   3.743553 0.8679239 5.475703 0.2086145 0.5609381 2.643509 0.2448524 0.06590444 0.4905917 0.04393452 0.13069757 2.7213780 0.07356250 0.09993333 0.0955000 -0.004433333 1.00000000 0.01793348 TRUE TRUE
MSTRG.14710.3 ENSG00000166263 HEK293_OSMI2_2hA HEK293_TMG_2hB STXBP4 protein_coding   3.743553 0.8679239 5.475703 0.2086145 0.5609381 2.643509 1.7242243 0.09246611 2.7847063 0.09246611 0.21843235 4.7694779 0.29247500 0.07193333 0.5179333 0.446000000 0.02542486 0.01793348 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000166263 E001 1.0351353 0.1234599476 6.161194e-01 7.342297e-01 17 54968727 54968764 38 + 0.236 0.389 1.010
ENSG00000166263 E002 1.9175973 0.0161343708 7.751543e-01 8.551203e-01 17 54968765 54968774 10 + 0.387 0.505 0.610
ENSG00000166263 E003 1.9175973 0.0161343708 7.751543e-01 8.551203e-01 17 54968775 54968775 1 + 0.387 0.505 0.610
ENSG00000166263 E004 5.1596176 0.0082474483 7.621169e-01 8.457960e-01 17 54968776 54968791 16 + 0.702 0.733 0.128
ENSG00000166263 E005 5.3111330 0.0042288573 6.976865e-01 7.977560e-01 17 54968792 54968794 3 + 0.714 0.733 0.078
ENSG00000166263 E006 9.6729646 0.0120005781 4.257436e-01 5.686557e-01 17 54968795 54968815 21 + 0.890 1.080 0.705
ENSG00000166263 E007 15.2603892 0.0012193527 8.906788e-01 9.341913e-01 17 54985614 54985691 78 + 1.098 1.175 0.277
ENSG00000166263 E008 19.6479286 0.0009546075 4.868225e-02 1.068271e-01 17 54986142 54986266 125 + 1.234 1.130 -0.369
ENSG00000166263 E009 21.7971815 0.0008642245 3.409785e-04 1.544135e-03 17 54990825 54990957 133 + 1.301 1.023 -0.993
ENSG00000166263 E010 0.0000000       17 54990958 54990988 31 +      
ENSG00000166263 E011 0.6172225 0.4152198329 7.057781e-01 8.040868e-01 17 54991039 54991852 814 + 0.155 0.240 0.781
ENSG00000166263 E012 22.7128862 0.0027463150 1.499096e-02 4.046727e-02 17 54999345 54999451 107 + 1.298 1.154 -0.511
ENSG00000166263 E013 0.2214452 0.0369907955 1.044097e-01   17 54999626 54999631 6 + 0.000 0.240 12.222
ENSG00000166263 E014 27.8803858 0.0006984547 4.545835e-03 1.466898e-02 17 54999632 54999815 184 + 1.386 1.235 -0.529
ENSG00000166263 E015 11.3309553 0.0015288787 7.499132e-02 1.515207e-01 17 54999816 54999842 27 + 1.022 0.882 -0.523
ENSG00000166263 E016 16.5754694 0.0010773752 5.455181e-03 1.716845e-02 17 55000808 55000883 76 + 1.184 0.958 -0.819
ENSG00000166263 E017 16.9808356 0.0017680108 3.930417e-02 8.973315e-02 17 55007506 55007597 92 + 1.184 1.053 -0.471
ENSG00000166263 E018 0.0000000       17 55030807 55031167 361 +      
ENSG00000166263 E019 18.8715653 0.0022193343 4.257108e-02 9.576522e-02 17 55031168 55031264 97 + 1.223 1.106 -0.418
ENSG00000166263 E020 17.8938402 0.0010735338 1.992166e-02 5.132263e-02 17 55034168 55034259 92 + 1.208 1.053 -0.556
ENSG00000166263 E021 17.6754274 0.0010819279 2.731123e-04 1.270262e-03 17 55043236 55043325 90 + 1.219 0.882 -1.233
ENSG00000166263 E022 0.2966881 0.0290785164 1.000000e+00   17 55043326 55043375 50 + 0.110 0.000 -11.065
ENSG00000166263 E023 6.0182929 0.0205126140 1.454654e-02 3.946890e-02 17 55043612 55044776 1165 + 0.649 1.077 1.662
ENSG00000166263 E024 14.5115535 0.0011941586 5.467744e-03 1.720193e-02 17 55047089 55047154 66 + 1.131 0.882 -0.922
ENSG00000166263 E025 20.2773163 0.0009601832 1.081331e-01 2.027724e-01 17 55072900 55073076 177 + 1.238 1.175 -0.221
ENSG00000166263 E026 13.8181922 0.0012789282 4.561369e-01 5.966071e-01 17 55078078 55078194 117 + 1.072 1.080 0.029
ENSG00000166263 E027 9.5180452 0.0100857486 9.434272e-01 9.685186e-01 17 55078686 55078735 50 + 0.914 0.992 0.290
ENSG00000166263 E028 20.7833570 0.0012031437 9.264448e-02 1.792587e-01 17 55081050 55081183 134 + 1.245 1.175 -0.245
ENSG00000166263 E029 17.2733259 0.0010718894 3.407132e-01 4.846497e-01 17 55141310 55141367 58 + 1.158 1.153 -0.017
ENSG00000166263 E030 22.7972402 0.0010102992 7.094690e-03 2.147450e-02 17 55159797 55159946 150 + 1.298 1.130 -0.594
ENSG00000166263 E031 13.2606077 0.0055796938 1.012262e-01 1.923432e-01 17 55159947 55160050 104 + 1.072 0.959 -0.415
ENSG00000166263 E032 274.2910042 0.0083462550 4.236920e-12 9.375911e-11 17 55160051 55173632 13582 + 2.257 2.564 1.021
ENSG00000166263 E033 2.2445761 0.0063369344 5.174491e-01 6.518287e-01 17 55175859 55176069 211 + 0.458 0.393 -0.350
ENSG00000166263 E034 0.4396707 0.0274484742 6.169935e-01 7.349043e-01 17 55201588 55201708 121 + 0.156 0.000 -11.615