ENSG00000166262

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000558579 ENSG00000166262 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM227B protein_coding protein_coding 1.300745 0.652535 1.984779 0.03103074 0.3646042 1.59016 0.43761376 0.40905528 0.61610194 0.03252999 0.09326218 0.5792570 0.38570833 0.62493333 0.31636667 -0.30856667 0.004567922 0.004567922 FALSE FALSE
ENST00000558594 ENSG00000166262 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM227B protein_coding protein_coding 1.300745 0.652535 1.984779 0.03103074 0.3646042 1.59016 0.09577553 0.03069855 0.19143002 0.03069855 0.03259394 2.3072296 0.09334167 0.04473333 0.10623333 0.06150000 0.613279330 0.004567922 FALSE FALSE
ENST00000558862 ENSG00000166262 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM227B protein_coding processed_transcript 1.300745 0.652535 1.984779 0.03103074 0.3646042 1.59016 0.09257769 0.00000000 0.12085269 0.00000000 0.07487242 3.7098717 0.05755833 0.00000000 0.07550000 0.07550000 0.565178355 0.004567922   FALSE
ENST00000560246 ENSG00000166262 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM227B protein_coding protein_coding 1.300745 0.652535 1.984779 0.03103074 0.3646042 1.59016 0.09956022 0.00000000 0.18802725 0.00000000 0.18802725 4.3076271 0.05051667 0.00000000 0.06933333 0.06933333 1.000000000 0.004567922 FALSE TRUE
ENST00000561064 ENSG00000166262 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM227B protein_coding protein_coding 1.300745 0.652535 1.984779 0.03103074 0.3646042 1.59016 0.02648019 0.00000000 0.10728791 0.00000000 0.03825766 3.5519825 0.01610833 0.00000000 0.05790000 0.05790000 0.201002158 0.004567922 FALSE TRUE
ENST00000561116 ENSG00000166262 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM227B protein_coding nonsense_mediated_decay 1.300745 0.652535 1.984779 0.03103074 0.3646042 1.59016 0.08478084 0.05463719 0.07907832 0.05463719 0.03961540 0.4627098 0.06892917 0.08026667 0.03866667 -0.04160000 0.960807610 0.004567922   FALSE
ENST00000561319 ENSG00000166262 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM227B protein_coding retained_intron 1.300745 0.652535 1.984779 0.03103074 0.3646042 1.59016 0.03403051 0.06157471 0.07261279 0.06157471 0.07261279 0.2069151 0.04251667 0.10426667 0.02676667 -0.07750000 0.871531877 0.004567922 FALSE TRUE
MSTRG.10778.13 ENSG00000166262 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM227B protein_coding   1.300745 0.652535 1.984779 0.03103074 0.3646042 1.59016 0.24448359 0.00000000 0.42125640 0.00000000 0.22911777 5.4304740 0.13636667 0.00000000 0.19206667 0.19206667 0.330695185 0.004567922 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000166262 E001 0.0000000       15 49326962 49326969 8 -      
ENSG00000166262 E002 0.4470576 0.024533001 0.792805494 0.867514998 15 49326970 49328392 1423 - 0.149 0.181 0.339
ENSG00000166262 E003 0.6717251 0.021453245 0.966546310 0.983031614 15 49328393 49328675 283 - 0.208 0.182 -0.242
ENSG00000166262 E004 0.5202097 0.023174508 0.790129313 0.865766865 15 49331780 49331849 70 - 0.149 0.182 0.343
ENSG00000166262 E005 0.0000000       15 49334218 49334302 85 -      
ENSG00000166262 E006 0.1472490 0.045346353 1.000000000   15 49335419 49335496 78 - 0.081 0.000 -11.732
ENSG00000166262 E007 0.2924217 0.029908950 0.650293780   15 49367448 49367608 161 - 0.149 0.000 -12.624
ENSG00000166262 E008 0.1515154 0.044765646 1.000000000   15 49371302 49371399 98 - 0.081 0.000 -11.732
ENSG00000166262 E009 0.0000000       15 49395975 49396097 123 -      
ENSG00000166262 E010 0.0000000       15 49396244 49396442 199 -      
ENSG00000166262 E011 0.2987644 0.031358545 0.645052582   15 49422400 49422714 315 - 0.149 0.000 -12.625
ENSG00000166262 E012 0.0000000       15 49489061 49489391 331 -      
ENSG00000166262 E013 0.2966881 0.029536648 0.650773301   15 49508211 49508348 138 - 0.149 0.000 -12.625
ENSG00000166262 E014 1.0499988 0.214244030 0.123319550 0.224700101 15 49541680 49541806 127 - 0.387 0.000 -14.160
ENSG00000166262 E015 0.6060617 1.005014731 0.714313038 0.810559095 15 49568245 49568346 102 - 0.262 0.000 -13.442
ENSG00000166262 E016 0.1451727 0.042461988 1.000000000   15 49574738 49574860 123 - 0.081 0.000 -11.731
ENSG00000166262 E017 0.2214452 0.039231204 0.170796703   15 49574861 49575010 150 - 0.000 0.182 13.634
ENSG00000166262 E018 1.2910239 0.010784990 0.914587184 0.949948180 15 49575011 49575109 99 - 0.348 0.309 -0.242
ENSG00000166262 E019 6.4463209 0.003220097 0.033272050 0.078333852 15 49576136 49576493 358 - 0.771 0.991 0.843
ENSG00000166262 E020 3.5375825 0.004728822 0.002830163 0.009764805 15 49576494 49576559 66 - 0.453 0.858 1.757
ENSG00000166262 E021 4.9175658 0.004189306 0.016359506 0.043518533 15 49576560 49576740 181 - 0.631 0.917 1.149
ENSG00000166262 E022 3.3215391 0.004898269 0.492528768 0.629693401 15 49576741 49576760 20 - 0.587 0.665 0.341
ENSG00000166262 E023 4.8996992 0.003602008 0.477450337 0.615991215 15 49576761 49576845 85 - 0.724 0.791 0.271
ENSG00000166262 E024 1.2867575 0.011306623 0.914376684 0.949792029 15 49577172 49577302 131 - 0.348 0.309 -0.242
ENSG00000166262 E025 0.5827671 0.022256571 0.247639479 0.383169860 15 49577303 49577305 3 - 0.259 0.000 -13.490
ENSG00000166262 E026 3.0916748 0.005714756 0.731214173 0.822923614 15 49577629 49577664 36 - 0.587 0.613 0.118
ENSG00000166262 E027 4.4913397 0.004041397 0.756955591 0.841919674 15 49588016 49588083 68 - 0.740 0.665 -0.311
ENSG00000166262 E028 7.3282333 0.002536614 0.210078548 0.338640720 15 49589776 49590007 232 - 0.943 0.753 -0.736
ENSG00000166262 E029 0.5975289 0.098359291 0.254214429 0.390862809 15 49606156 49606218 63 - 0.260 0.000 -13.487
ENSG00000166262 E030 4.7544909 0.003979365 0.152914601 0.265623939 15 49611215 49611268 54 - 0.800 0.555 -1.037
ENSG00000166262 E031 0.0000000       15 49614792 49615120 329 -      
ENSG00000166262 E032 7.0144941 0.011155574 0.141581444 0.250192899 15 49615121 49615243 123 - 0.943 0.711 -0.905
ENSG00000166262 E033 0.6256415 0.018230863 0.972443271 0.986814688 15 49620134 49620190 57 - 0.208 0.181 -0.243
ENSG00000166262 E034 1.7985626 0.008880441 0.413819130 0.557218857 15 49620631 49620699 69 - 0.483 0.309 -0.981
ENSG00000166262 E035 3.1500676 0.058576567 0.250263815 0.386248294 15 49620700 49620929 230 - 0.669 0.407 -1.243