ENSG00000166246

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000299320 ENSG00000166246 HEK293_OSMI2_2hA HEK293_TMG_2hB DNAAF8 protein_coding protein_coding 1.310714 3.095433 0.3981105 0.107906 0.05526258 -2.927763 0.1850307 0.4031388 0.14917919 0.04598131 0.03671545 -1.375975 0.2035542 0.1308333 0.3839 0.25306667 0.02365493 0.02365493 FALSE TRUE
ENST00000586724 ENSG00000166246 HEK293_OSMI2_2hA HEK293_TMG_2hB DNAAF8 protein_coding protein_coding 1.310714 3.095433 0.3981105 0.107906 0.05526258 -2.927763 0.4043197 0.9156562 0.06455178 0.17693189 0.06455178 -3.634162 0.2513625 0.3000000 0.1473 -0.15270000 0.47970353 0.02365493 FALSE FALSE
ENST00000619274 ENSG00000166246 HEK293_OSMI2_2hA HEK293_TMG_2hB DNAAF8 protein_coding protein_coding 1.310714 3.095433 0.3981105 0.107906 0.05526258 -2.927763 0.6169596 1.5927554 0.18437950 0.30011130 0.03239394 -3.043606 0.4737083 0.5090333 0.4688 -0.04023333 0.94991907 0.02365493 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000166246 E001 1.9604665 0.013409945 0.81461000 0.88255551 16 4734344 4734372 29 + 0.275 0.342 0.442
ENSG00000166246 E002 1.4714926 0.020761396 1.00000000 1.00000000 16 4734480 4734501 22 + 0.275 0.291 0.114
ENSG00000166246 E003 1.2177675 0.012157074 0.44475557 0.58631527 16 4734502 4734536 35 + 0.000 0.265 11.958
ENSG00000166246 E004 7.8225101 0.002410563 0.10530904 0.19856164 16 4734537 4734698 162 + 0.442 0.772 1.479
ENSG00000166246 E005 9.6679083 0.002027678 0.54662031 0.67677531 16 4736464 4736643 180 + 0.734 0.845 0.442
ENSG00000166246 E006 11.2877943 0.001912133 0.56597924 0.69300916 16 4737800 4737946 147 + 0.799 0.901 0.393
ENSG00000166246 E007 0.0000000       16 4737947 4737988 42 +      
ENSG00000166246 E008 21.1740194 0.001361600 0.07214876 0.14691164 16 4740153 4740503 351 + 1.271 1.122 -0.530
ENSG00000166246 E009 14.6385326 0.001516763 0.80367922 0.87506234 16 4740504 4740659 156 + 0.993 0.979 -0.051
ENSG00000166246 E010 10.9814012 0.036658323 0.78165094 0.85971393 16 4743043 4743160 118 + 0.799 0.876 0.297
ENSG00000166246 E011 20.7077642 0.008133366 0.47196857 0.61104943 16 4743161 4743839 679 + 1.030 1.134 0.378
ENSG00000166246 E012 0.1817044 0.041914714 0.71347059   16 4744870 4745011 142 + 0.000 0.049 9.615
ENSG00000166246 E013 1.2114379 0.011277352 0.44343374 0.58501645 16 4746375 4746512 138 + 0.000 0.265 11.959
ENSG00000166246 E014 0.1817044 0.041914714 0.71347059   16 4746513 4746521 9 + 0.000 0.049 9.615
ENSG00000166246 E015 0.5451131 0.757347539 1.00000000 1.00000000 16 4746522 4746926 405 + 0.000 0.135 10.718
ENSG00000166246 E016 0.5514428 0.020080917 1.00000000 1.00000000 16 4746927 4747025 99 + 0.000 0.134 10.988
ENSG00000166246 E017 0.7268175 0.961777886 1.00000000 1.00000000 16 4747026 4747342 317 + 0.000 0.172 11.018
ENSG00000166246 E018 2.3218146 0.058119168 0.02408074 0.05997769 16 4747343 4747634 292 + 0.734 0.317 -2.037
ENSG00000166246 E019 1.3243307 0.010864776 0.44257887 0.58418440 16 4748243 4748724 482 + 0.000 0.265 11.961
ENSG00000166246 E020 1.6919954 0.008396616 0.02083661 0.05324327 16 4748725 4749396 672 + 0.656 0.235 -2.295