ENSG00000166225

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000397997 ENSG00000166225 HEK293_OSMI2_2hA HEK293_TMG_2hB FRS2 protein_coding protein_coding 4.602554 1.077508 9.044488 0.07100094 0.8462878 3.057607 0.097623050 0.40363308 0.00000000 0.20970461 0.00000000 -5.370280 0.084800000 0.36863333 0.0000 -0.36863333 0.0246346417 0.0007098705 FALSE TRUE
ENST00000548154 ENSG00000166225 HEK293_OSMI2_2hA HEK293_TMG_2hB FRS2 protein_coding protein_coding 4.602554 1.077508 9.044488 0.07100094 0.8462878 3.057607 0.176772496 0.00000000 0.67646804 0.00000000 0.11169726 6.101121 0.020466667 0.00000000 0.0774 0.07740000 0.0146939566 0.0007098705   FALSE
ENST00000549921 ENSG00000166225 HEK293_OSMI2_2hA HEK293_TMG_2hB FRS2 protein_coding protein_coding 4.602554 1.077508 9.044488 0.07100094 0.8462878 3.057607 3.575707181 0.30490401 6.95564541 0.11231037 0.27077993 4.467273 0.699212500 0.27690000 0.7771 0.50020000 0.0007098705 0.0007098705 FALSE TRUE
ENST00000550169 ENSG00000166225 HEK293_OSMI2_2hA HEK293_TMG_2hB FRS2 protein_coding protein_coding 4.602554 1.077508 9.044488 0.07100094 0.8462878 3.057607 0.009594523 0.07675619 0.00000000 0.07675619 0.00000000 -3.116967 0.009091667 0.07273333 0.0000 -0.07273333 0.3845197576 0.0007098705   FALSE
ENST00000550389 ENSG00000166225 HEK293_OSMI2_2hA HEK293_TMG_2hB FRS2 protein_coding protein_coding 4.602554 1.077508 9.044488 0.07100094 0.8462878 3.057607 0.058994474 0.23589428 0.05094031 0.23589428 0.05094031 -2.012569 0.050179167 0.22350000 0.0060 -0.21750000 0.7285798779 0.0007098705 FALSE TRUE
ENST00000551325 ENSG00000166225 HEK293_OSMI2_2hA HEK293_TMG_2hB FRS2 protein_coding protein_coding 4.602554 1.077508 9.044488 0.07100094 0.8462878 3.057607 0.158335851 0.00000000 0.69605272 0.00000000 0.69605272 6.141704 0.015279167 0.00000000 0.0650 0.06500000 1.0000000000 0.0007098705 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000166225 E001 0.1515154 0.0435645082 7.505639e-01   12 69470349 69470377 29 + 0.049 0.000 -7.138
ENSG00000166225 E002 0.2987644 0.0276006801 1.000000e+00   12 69470378 69470382 5 + 0.093 0.000 -9.390
ENSG00000166225 E003 0.2987644 0.0276006801 1.000000e+00   12 69470383 69470387 5 + 0.093 0.000 -9.390
ENSG00000166225 E004 0.2987644 0.0276006801 1.000000e+00   12 69470388 69470391 4 + 0.093 0.000 -9.390
ENSG00000166225 E005 3.2781156 0.0454428008 6.744126e-01 7.799040e-01 12 69470392 69470405 14 + 0.531 0.441 -0.446
ENSG00000166225 E006 5.7859566 0.0028043259 1.405894e-01 2.488367e-01 12 69470406 69470407 2 + 0.735 0.442 -1.324
ENSG00000166225 E007 8.6689483 0.0019587271 7.447978e-02 1.507025e-01 12 69470408 69470419 12 + 0.880 0.563 -1.310
ENSG00000166225 E008 13.1698305 0.0012913523 1.488984e-02 4.023901e-02 12 69470420 69470433 14 + 1.043 0.657 -1.506
ENSG00000166225 E009 21.8112178 0.0008535806 2.471555e-03 8.678293e-03 12 69470434 69470530 97 + 1.246 0.857 -1.425
ENSG00000166225 E010 0.8909948 0.0136968632 7.380294e-01 8.280229e-01 12 69480299 69480359 61 + 0.204 0.275 0.563
ENSG00000166225 E011 1.0425102 0.0118864467 8.750446e-01 9.237102e-01 12 69486202 69486297 96 + 0.235 0.275 0.300
ENSG00000166225 E012 1.0966340 0.1618111302 8.100347e-01 8.794242e-01 12 69491538 69491651 114 + 0.234 0.278 0.322
ENSG00000166225 E013 27.7748410 0.0006830950 2.165359e-04 1.034892e-03 12 69530865 69530960 96 + 1.348 0.907 -1.590
ENSG00000166225 E014 18.1934181 0.0015222677 2.164569e-03 7.733834e-03 12 69532014 69532056 43 + 1.176 0.734 -1.663
ENSG00000166225 E015 2.5957799 0.0165201390 9.782230e-01 9.905104e-01 12 69537916 69537983 68 + 0.447 0.443 -0.021
ENSG00000166225 E016 0.0000000       12 69557789 69557799 11 +      
ENSG00000166225 E017 0.5891098 0.0183573426 1.000000e+00 1.000000e+00 12 69557800 69557856 57 + 0.170 0.000 -10.430
ENSG00000166225 E018 0.0000000       12 69562148 69562179 32 +      
ENSG00000166225 E019 29.6344609 0.0043947740 1.403120e-01 2.484525e-01 12 69562180 69562274 95 + 1.353 1.205 -0.520
ENSG00000166225 E020 27.4683829 0.0006740959 5.118775e-02 1.112696e-01 12 69569005 69569096 92 + 1.327 1.127 -0.707
ENSG00000166225 E021 42.1709553 0.0005880390 2.028496e-02 5.208061e-02 12 69570331 69570517 187 + 1.505 1.311 -0.671
ENSG00000166225 E022 42.1795477 0.0006593826 2.315527e-02 5.807194e-02 12 69571276 69571434 159 + 1.502 1.311 -0.659
ENSG00000166225 E023 45.4932554 0.0005386630 1.445393e-03 5.457739e-03 12 69572118 69572281 164 + 1.542 1.272 -0.936
ENSG00000166225 E024 562.5489930 0.0002660002 9.536308e-20 5.880940e-18 12 69574005 69579793 5789 + 2.578 2.687 0.363