ENSG00000166128

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000321437 ENSG00000166128 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB8B protein_coding protein_coding 3.537464 0.6041671 6.480731 0.1500503 0.2831788 3.401678 1.73803392 0.52326073 3.363587 0.14687523 0.4000929 2.661371 0.62732083 0.85763333 0.5209 -0.33673333 3.795464e-03 1.047422e-07 FALSE TRUE
ENST00000558119 ENSG00000166128 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB8B protein_coding nonsense_mediated_decay 3.537464 0.6041671 6.480731 0.1500503 0.2831788 3.401678 0.02600805 0.04056039 0.000000 0.04056039 0.0000000 -2.338008 0.02239583 0.07666667 0.0000 -0.07666667 2.804954e-01 1.047422e-07 FALSE TRUE
ENST00000559006 ENSG00000166128 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB8B protein_coding protein_coding 3.537464 0.6041671 6.480731 0.1500503 0.2831788 3.401678 1.23278211 0.00000000 2.102632 0.00000000 0.1177102 7.722898 0.19666250 0.00000000 0.3243 0.32430000 1.047422e-07 1.047422e-07 FALSE FALSE
MSTRG.10985.1 ENSG00000166128 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB8B protein_coding   3.537464 0.6041671 6.480731 0.1500503 0.2831788 3.401678 0.43896181 0.04034600 0.693859 0.02266031 0.5295535 3.805337 0.13780000 0.06570000 0.1054 0.03970000 1.000000e+00 1.047422e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000166128 E001 0.1515154 0.0445107997 0.946311266   15 63189534 63189559 26 + 0.054 0.000 -7.850
ENSG00000166128 E002 2.4151197 0.0069066762 0.851543939 0.90794659 15 63189560 63189593 34 + 0.454 0.416 -0.202
ENSG00000166128 E003 3.3941420 0.0819210789 0.947599778 0.97103495 15 63189594 63189602 9 + 0.546 0.533 -0.060
ENSG00000166128 E004 3.6865637 0.0423033226 0.876242783 0.92456803 15 63189603 63189605 3 + 0.577 0.533 -0.203
ENSG00000166128 E005 18.8977315 0.0014739109 0.029933478 0.07182965 15 63189606 63189748 143 + 1.212 0.956 -0.931
ENSG00000166128 E006 0.2987644 0.0278722552 1.000000000   15 63223042 63223073 32 + 0.102 0.000 -10.325
ENSG00000166128 E007 0.2924217 0.0283270728 1.000000000   15 63223848 63223979 132 + 0.102 0.000 -10.322
ENSG00000166128 E008 17.3706118 0.0010953076 0.065610609 0.13605969 15 63244756 63244816 61 + 1.176 0.956 -0.801
ENSG00000166128 E009 18.3370624 0.0010448890 0.081428191 0.16179075 15 63249645 63249705 61 + 1.194 0.993 -0.729
ENSG00000166128 E010 0.0000000       15 63251284 63251342 59 +      
ENSG00000166128 E011 17.7675299 0.0011092393 0.678416012 0.78288498 15 63255507 63255584 78 + 1.160 1.115 -0.160
ENSG00000166128 E012 18.1324607 0.0011024038 0.535506877 0.66732075 15 63256505 63256594 90 + 1.148 1.211 0.224
ENSG00000166128 E013 18.6549768 0.0015656579 0.960075233 0.97890892 15 63259627 63259692 66 + 1.172 1.166 -0.021
ENSG00000166128 E014 14.1265257 0.0013204231 0.308349943 0.45062578 15 63262692 63262742 51 + 1.082 0.956 -0.465
ENSG00000166128 E015 0.1451727 0.0457905404 0.947106105   15 63262743 63262776 34 + 0.054 0.000 -9.247
ENSG00000166128 E016 236.1039251 0.0003500344 0.002966348 0.01016268 15 63263527 63267776 4250 + 2.241 2.295 0.181