ENSG00000165916

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000298852 ENSG00000165916 HEK293_OSMI2_2hA HEK293_TMG_2hB PSMC3 protein_coding protein_coding 202.5814 295.2504 160.9964 31.41407 2.755847 -0.8748699 139.29460 229.11048 94.68846 23.877356 17.4699124 -1.2746936 0.6675500 0.7782000 0.5873000 -0.19090000 3.179102e-01 4.838434e-05 FALSE  
ENST00000530887 ENSG00000165916 HEK293_OSMI2_2hA HEK293_TMG_2hB PSMC3 protein_coding nonsense_mediated_decay 202.5814 295.2504 160.9964 31.41407 2.755847 -0.8748699 14.88611 12.61992 16.97414 2.876845 0.6289745 0.4273422 0.0814500 0.0416000 0.1054667 0.06386667 4.838434e-05 4.838434e-05 FALSE  
ENST00000602866 ENSG00000165916 HEK293_OSMI2_2hA HEK293_TMG_2hB PSMC3 protein_coding protein_coding 202.5814 295.2504 160.9964 31.41407 2.755847 -0.8748699 41.78573 46.99216 40.96814 9.871281 17.4760849 -0.1978725 0.2155417 0.1577667 0.2553000 0.09753333 8.777641e-01 4.838434e-05 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000165916 E001 6.217418 1.164575e-02 4.090027e-01 5.526070e-01 11 47418769 47418774 6 - 0.732 0.849 0.464
ENSG00000165916 E002 275.183898 2.202033e-04 2.263839e-05 1.385935e-04 11 47418775 47418801 27 - 2.318 2.424 0.353
ENSG00000165916 E003 1230.787504 7.840883e-04 1.988456e-05 1.234255e-04 11 47418802 47418945 144 - 2.990 3.060 0.233
ENSG00000165916 E004 1401.191711 3.930949e-04 4.779232e-09 6.260292e-08 11 47419116 47419197 82 - 3.040 3.120 0.264
ENSG00000165916 E005 1618.274833 3.885322e-04 1.545787e-08 1.839362e-07 11 47420264 47420338 75 - 3.108 3.181 0.243
ENSG00000165916 E006 1606.872059 1.962594e-04 9.834308e-14 2.797418e-12 11 47420339 47420409 71 - 3.097 3.182 0.284
ENSG00000165916 E007 1631.935888 1.379241e-04 4.230748e-11 7.948260e-10 11 47420631 47420727 97 - 3.115 3.185 0.235
ENSG00000165916 E008 1849.057935 1.361740e-03 1.434584e-03 5.421674e-03 11 47422574 47422722 149 - 3.177 3.230 0.176
ENSG00000165916 E009 1687.533209 3.178082e-04 6.152083e-02 1.291907e-01 11 47422830 47422932 103 - 3.167 3.184 0.059
ENSG00000165916 E010 1354.365330 6.875781e-05 3.617599e-03 1.206081e-02 11 47422933 47422973 41 - 3.110 3.078 -0.107
ENSG00000165916 E011 1815.430440 2.571683e-04 3.264494e-04 1.486655e-03 11 47424046 47424107 62 - 3.244 3.200 -0.147
ENSG00000165916 E012 1198.749409 5.405218e-04 1.036101e-04 5.386845e-04 11 47424108 47424108 1 - 3.077 3.013 -0.212
ENSG00000165916 E013 1764.048690 4.338494e-04 1.992200e-04 9.604690e-04 11 47424109 47424183 75 - 3.237 3.184 -0.176
ENSG00000165916 E014 9.715508 1.991320e-03 6.867548e-07 5.907626e-06 11 47424364 47424422 59 - 1.291 0.777 -1.895
ENSG00000165916 E015 8.951954 2.119352e-03 2.006339e-05 1.244092e-04 11 47424423 47424428 6 - 1.233 0.777 -1.691
ENSG00000165916 E016 1559.768222 3.350075e-04 1.763069e-04 8.624236e-04 11 47424429 47424491 63 - 3.183 3.132 -0.168
ENSG00000165916 E017 9.643303 1.671212e-02 2.046582e-01 3.319710e-01 11 47424492 47424606 115 - 1.087 0.925 -0.600
ENSG00000165916 E018 2085.292360 5.437483e-04 7.662020e-07 6.528206e-06 11 47424607 47424711 105 - 3.321 3.251 -0.234
ENSG00000165916 E019 2317.970562 4.433096e-04 1.300974e-03 4.979709e-03 11 47425121 47425246 126 - 3.349 3.304 -0.150
ENSG00000165916 E020 2.907865 1.560407e-01 9.674700e-02 1.855010e-01 11 47425439 47425866 428 - 0.769 0.411 -1.628
ENSG00000165916 E021 1721.061913 8.719594e-04 4.851623e-01 6.230979e-01 11 47425867 47425946 80 - 3.201 3.180 -0.070
ENSG00000165916 E022 891.574924 1.921679e-03 6.210580e-03 1.916872e-02 11 47425947 47425950 4 - 2.956 2.878 -0.260
ENSG00000165916 E023 25.069957 2.483273e-03 7.454510e-02 1.508107e-01 11 47426117 47426132 16 - 1.467 1.334 -0.461
ENSG00000165916 E024 24.682513 1.635073e-03 8.388982e-02 1.656350e-01 11 47426133 47426204 72 - 1.460 1.334 -0.436
ENSG00000165916 E025 1180.632984 3.212804e-03 2.428332e-01 3.775054e-01 11 47426205 47426473 269 - 3.055 3.008 -0.159