ENSG00000165861

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000394207 ENSG00000165861 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFYVE1 protein_coding protein_coding 6.808916 9.08494 4.816801 1.138009 0.2160368 -0.913997 0.6097362 0.6303574 0.0000000 0.6303574 0.00000000 -6.000805 0.06583333 0.07450000 0.00000000 -0.07450000 8.790820e-01 3.515193e-05 FALSE TRUE
ENST00000555072 ENSG00000165861 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFYVE1 protein_coding protein_coding 6.808916 9.08494 4.816801 1.138009 0.2160368 -0.913997 0.1257963 1.0063701 0.0000000 1.0063701 0.00000000 -6.667282 0.01113750 0.08910000 0.00000000 -0.08910000 8.843181e-01 3.515193e-05 FALSE TRUE
ENST00000556040 ENSG00000165861 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFYVE1 protein_coding retained_intron 6.808916 9.08494 4.816801 1.138009 0.2160368 -0.913997 0.1456249 0.0000000 0.3505333 0.0000000 0.13648382 5.172060 0.02213333 0.00000000 0.07206667 0.07206667 3.515193e-05 3.515193e-05 FALSE FALSE
ENST00000556143 ENSG00000165861 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFYVE1 protein_coding protein_coding 6.808916 9.08494 4.816801 1.138009 0.2160368 -0.913997 4.9945540 7.2529997 3.5660908 0.3996034 0.03912743 -1.022182 0.73998750 0.81813333 0.74350000 -0.07463333 7.819955e-01 3.515193e-05 FALSE TRUE
MSTRG.9849.4 ENSG00000165861 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFYVE1 protein_coding   6.808916 9.08494 4.816801 1.138009 0.2160368 -0.913997 0.5999867 0.1300822 0.7350710 0.1300822 0.03221696 2.411104 0.10819167 0.01153333 0.15260000 0.14106667 1.287015e-02 3.515193e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000165861 E001 0.8062992 0.0409687153 1.783571e-01 0.2990805588 14 72969451 72969451 1 - 0.000 0.275 10.561
ENSG00000165861 E002 58.6993050 0.0004648912 2.665730e-05 0.0001605259 14 72969452 72969756 305 - 1.548 1.776 0.775
ENSG00000165861 E003 185.7353397 0.0016096626 3.132873e-05 0.0001855346 14 72969757 72970710 954 - 2.116 2.255 0.466
ENSG00000165861 E004 66.1940534 0.0070999060 9.715121e-01 0.9861874048 14 72970711 72970877 167 - 1.771 1.772 0.004
ENSG00000165861 E005 33.2224480 0.0127669306 7.056968e-01 0.8040181635 14 72970878 72970881 4 - 1.497 1.470 -0.096
ENSG00000165861 E006 34.1029459 0.0152580735 6.032433e-01 0.7240685001 14 72970882 72970892 11 - 1.517 1.477 -0.136
ENSG00000165861 E007 33.9585438 0.0006981131 5.128300e-01 0.6477243162 14 72970893 72970929 37 - 1.517 1.476 -0.141
ENSG00000165861 E008 73.2072700 0.0003705303 7.639248e-01 0.8471274692 14 72970930 72971114 185 - 1.808 1.823 0.049
ENSG00000165861 E009 0.9180738 0.1149711830 6.206621e-01 0.7377679848 14 72974078 72974079 2 - 0.171 0.276 0.873
ENSG00000165861 E010 78.6647961 0.0004342720 1.748763e-01 0.2945184006 14 72974080 72974193 114 - 1.808 1.869 0.204
ENSG00000165861 E011 56.7559925 0.0004390045 1.628270e-01 0.2788261606 14 72974779 72974827 49 - 1.658 1.731 0.248
ENSG00000165861 E012 85.4499547 0.0005553858 2.963816e-01 0.4375773447 14 72974828 72974959 132 - 1.855 1.900 0.152
ENSG00000165861 E013 0.6653823 0.0170730246 7.507570e-02 0.1516558003 14 72974960 72975207 248 - 0.389 0.086 -2.725
ENSG00000165861 E014 77.2789018 0.0070183463 6.428166e-01 0.7555253231 14 72975551 72975721 171 - 1.860 1.838 -0.074
ENSG00000165861 E015 1.4037409 0.0103394951 4.064599e-01 0.5501050243 14 72975722 72975941 220 - 0.467 0.321 -0.822
ENSG00000165861 E016 41.8833289 0.0006109396 5.683908e-01 0.6950859145 14 72977927 72977963 37 - 1.609 1.577 -0.107
ENSG00000165861 E017 42.0581868 0.0005412055 4.819780e-01 0.6201475865 14 72977964 72978002 39 - 1.614 1.575 -0.133
ENSG00000165861 E018 41.3999208 0.0005166205 5.186328e-01 0.6528727098 14 72978003 72978044 42 - 1.548 1.588 0.135
ENSG00000165861 E019 50.6015656 0.0004573055 9.470275e-01 0.9707365076 14 72978137 72978234 98 - 1.662 1.660 -0.008
ENSG00000165861 E020 50.2968322 0.0005142508 1.247270e-01 0.2267520844 14 72978861 72978969 109 - 1.598 1.684 0.291
ENSG00000165861 E021 1.6136320 0.0100413861 8.924700e-01 0.9352871191 14 72978970 72979225 256 - 0.389 0.365 -0.139
ENSG00000165861 E022 49.4597664 0.0004647615 7.725445e-01 0.8531862570 14 72981789 72981895 107 - 1.639 1.656 0.058
ENSG00000165861 E023 0.1451727 0.0426895235 2.216608e-01   14 72986892 72986896 5 - 0.171 0.000 -13.287
ENSG00000165861 E024 0.1451727 0.0426895235 2.216608e-01   14 72986897 72986985 89 - 0.171 0.000 -13.287
ENSG00000165861 E025 62.9676643 0.0004450622 4.742178e-01 0.6130630697 14 72993143 72993357 215 - 1.774 1.742 -0.111
ENSG00000165861 E026 104.4642768 0.0003255753 1.104384e-02 0.0312654719 14 72997811 72998315 505 - 2.037 1.947 -0.302
ENSG00000165861 E027 146.1886394 0.0003105929 3.587203e-05 0.0002091352 14 73024026 73024861 836 - 2.200 2.078 -0.410
ENSG00000165861 E028 29.8775331 0.0010916651 1.473297e-03 0.0055500431 14 73024862 73024942 81 - 1.577 1.369 -0.716
ENSG00000165861 E029 32.1244338 0.0006948347 6.850500e-03 0.0208379217 14 73026926 73027146 221 - 1.588 1.418 -0.581