ENSG00000165821

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000537235 ENSG00000165821 HEK293_OSMI2_2hA HEK293_TMG_2hB SALL2 protein_coding protein_coding 15.18506 22.81137 10.13595 0.4504146 0.08140198 -1.169481 12.515830 16.731911 9.6952345 1.203934 0.06037858 -0.7866292 0.8673167 0.7330000 0.9567 0.2237000 2.256232e-05 2.256232e-05 FALSE TRUE
ENST00000613414 ENSG00000165821 HEK293_OSMI2_2hA HEK293_TMG_2hB SALL2 protein_coding protein_coding 15.18506 22.81137 10.13595 0.4504146 0.08140198 -1.169481 2.399068 5.842941 0.1799701 1.127102 0.09828627 -4.9453173 0.1129375 0.2568667 0.0176 -0.2392667 1.250137e-03 2.256232e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000165821 E001 2.139894 0.0650343894 7.153258e-02 1.458843e-01 14 21521080 21521080 1 - 0.175 0.547 2.352
ENSG00000165821 E002 2.139894 0.0650343894 7.153258e-02 1.458843e-01 14 21521081 21521082 2 - 0.175 0.547 2.352
ENSG00000165821 E003 269.144327 0.0059542855 7.440194e-05 4.012875e-04 14 21521083 21521519 437 - 2.252 2.415 0.545
ENSG00000165821 E004 125.039502 0.0067931086 1.682264e-01 2.859012e-01 14 21521520 21521536 17 - 1.998 2.064 0.220
ENSG00000165821 E005 217.754974 0.0056470053 6.698923e-02 1.383673e-01 14 21521537 21521621 85 - 2.231 2.304 0.242
ENSG00000165821 E006 152.059709 0.0035918762 1.137760e-02 3.206706e-02 14 21521622 21521650 29 - 2.057 2.156 0.334
ENSG00000165821 E007 506.310791 0.0001435914 2.990311e-03 1.023608e-02 14 21521651 21522244 594 - 2.620 2.659 0.131
ENSG00000165821 E008 368.092632 0.0010779815 1.646190e-01 2.811835e-01 14 21522245 21522832 588 - 2.492 2.515 0.077
ENSG00000165821 E009 899.555434 0.0021197025 4.406077e-06 3.175402e-05 14 21522833 21524931 2099 - 2.966 2.872 -0.315
ENSG00000165821 E010 105.785968 0.0009570727 3.492699e-04 1.577211e-03 14 21524932 21525244 313 - 2.073 1.926 -0.493
ENSG00000165821 E011 41.997419 0.0098926079 7.887117e-01 8.647329e-01 14 21525245 21525336 92 - 1.564 1.575 0.037
ENSG00000165821 E012 118.448247 0.0066685404 3.466590e-03 1.162939e-02 14 21525337 21525654 318 - 2.131 1.973 -0.529
ENSG00000165821 E013 62.317286 0.0088037269 2.252654e-02 5.677486e-02 14 21526061 21526500 440 - 1.853 1.698 -0.524
ENSG00000165821 E014 0.000000       14 21536849 21536961 113 -      
ENSG00000165821 E015 0.000000       14 21536962 21537216 255 -