ENSG00000165757

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000375377 ENSG00000165757 HEK293_OSMI2_2hA HEK293_TMG_2hB JCAD protein_coding protein_coding 1.231606 0.9082644 1.528414 0.09724622 0.06227993 0.7444627 1.17559174 0.7444995 1.528414 0.02174790 0.06227993 1.027852 0.9447125 0.8365667 1 0.1634333 0.03309399 0.03309399 FALSE TRUE
ENST00000464386 ENSG00000165757 HEK293_OSMI2_2hA HEK293_TMG_2hB JCAD protein_coding processed_transcript 1.231606 0.9082644 1.528414 0.09724622 0.06227993 0.7444627 0.05601418 0.1637649 0.000000 0.08279713 0.00000000 -4.119065 0.0552875 0.1634333 0 -0.1634333 0.03711561 0.03309399   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000165757 E001 14.192689 0.0607663815 4.965445e-03 1.583384e-02 10 30012803 30013050 248 - 0.923 1.397 1.697
ENSG00000165757 E002 30.642746 0.0008013510 6.341464e-10 9.764865e-09 10 30013051 30013482 432 - 1.280 1.689 1.409
ENSG00000165757 E003 139.125891 0.0082787407 1.450488e-02 3.937505e-02 10 30013483 30017661 4179 - 2.091 2.204 0.377
ENSG00000165757 E004 16.920504 0.0011897760 2.517655e-02 6.223735e-02 10 30017662 30017917 256 - 1.308 1.156 -0.534
ENSG00000165757 E005 104.439974 0.0007369706 6.572590e-17 2.893696e-15 10 30026103 30029866 3764 - 2.100 1.876 -0.753
ENSG00000165757 E006 9.448231 0.0020067552 6.038761e-01 7.245434e-01 10 30047532 30047871 340 - 1.028 1.007 -0.075
ENSG00000165757 E007 2.385366 0.1178764485 7.464687e-01 8.342151e-01 10 30059482 30059586 105 - 0.548 0.490 -0.276
ENSG00000165757 E008 0.000000       10 30069700 30069821 122 -      
ENSG00000165757 E009 0.000000       10 30115367 30115494 128 -