ENSG00000165731

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000340058 ENSG00000165731 HEK293_OSMI2_2hA HEK293_TMG_2hB RET protein_coding protein_coding 2.020409 2.587492 1.577326 0.1608302 0.1160138 -0.7105208 0.17385348 0.4729792 0.00000000 0.18421690 0.00000000 -5.593889 0.06865833 0.17516667 0.00000000 -0.17516667 0.002324526 0.002324526 FALSE TRUE
ENST00000355710 ENSG00000165731 HEK293_OSMI2_2hA HEK293_TMG_2hB RET protein_coding protein_coding 2.020409 2.587492 1.577326 0.1608302 0.1160138 -0.7105208 0.47214145 0.2036769 0.64434229 0.12834980 0.08058594 1.614615 0.25992083 0.07413333 0.40653333 0.33240000 0.021626119 0.002324526 FALSE TRUE
ENST00000498820 ENSG00000165731 HEK293_OSMI2_2hA HEK293_TMG_2hB RET protein_coding protein_coding 2.020409 2.587492 1.577326 0.1608302 0.1160138 -0.7105208 0.12710453 0.0000000 0.00000000 0.00000000 0.00000000 0.000000 0.05385000 0.00000000 0.00000000 0.00000000   0.002324526 FALSE FALSE
ENST00000615310 ENSG00000165731 HEK293_OSMI2_2hA HEK293_TMG_2hB RET protein_coding protein_coding 2.020409 2.587492 1.577326 0.1608302 0.1160138 -0.7105208 0.07361539 0.0000000 0.21747960 0.00000000 0.08397745 4.507665 0.04600417 0.00000000 0.14550000 0.14550000 0.004114854 0.002324526 FALSE TRUE
ENST00000638465 ENSG00000165731 HEK293_OSMI2_2hA HEK293_TMG_2hB RET protein_coding protein_coding 2.020409 2.587492 1.577326 0.1608302 0.1160138 -0.7105208 0.06448155 0.3615731 0.00000000 0.18847792 0.00000000 -5.215574 0.02571250 0.13156667 0.00000000 -0.13156667 0.236991565 0.002324526 FALSE FALSE
ENST00000672389 ENSG00000165731 HEK293_OSMI2_2hA HEK293_TMG_2hB RET protein_coding nonsense_mediated_decay 2.020409 2.587492 1.577326 0.1608302 0.1160138 -0.7105208 0.04280017 0.0000000 0.11531571 0.00000000 0.11531571 3.647495 0.01934583 0.00000000 0.06733333 0.06733333 0.714098021 0.002324526 FALSE TRUE
ENST00000683007 ENSG00000165731 HEK293_OSMI2_2hA HEK293_TMG_2hB RET protein_coding retained_intron 2.020409 2.587492 1.577326 0.1608302 0.1160138 -0.7105208 0.10778714 0.2398931 0.00000000 0.14572375 0.00000000 -4.643239 0.06321250 0.10030000 0.00000000 -0.10030000 0.261168758 0.002324526 FALSE TRUE
ENST00000684216 ENSG00000165731 HEK293_OSMI2_2hA HEK293_TMG_2hB RET protein_coding protein_coding 2.020409 2.587492 1.577326 0.1608302 0.1160138 -0.7105208 0.22443587 0.1644588 0.02939303 0.16445882 0.02939303 -2.146874 0.10443750 0.07256667 0.01756667 -0.05500000 1.000000000 0.002324526 FALSE TRUE
MSTRG.3842.2 ENSG00000165731 HEK293_OSMI2_2hA HEK293_TMG_2hB RET protein_coding   2.020409 2.587492 1.577326 0.1608302 0.1160138 -0.7105208 0.09488500 0.2047036 0.05281095 0.11052541 0.05281095 -1.773258 0.05176250 0.08053333 0.03923333 -0.04130000 0.701536209 0.002324526 FALSE TRUE
MSTRG.3842.8 ENSG00000165731 HEK293_OSMI2_2hA HEK293_TMG_2hB RET protein_coding   2.020409 2.587492 1.577326 0.1608302 0.1160138 -0.7105208 0.55865438 0.9292236 0.40560061 0.04133281 0.06065079 -1.176271 0.26752917 0.36136667 0.25416667 -0.10720000 0.285936474 0.002324526 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000165731 E001 0.7686241 0.1023256147 0.1065785696 0.200501566 10 43077064 43077068 5 + 0.415 0.102 -2.599
ENSG00000165731 E002 0.7686241 0.1023256147 0.1065785696 0.200501566 10 43077069 43077073 5 + 0.415 0.102 -2.599
ENSG00000165731 E003 1.7413037 0.0084103110 0.0065770431 0.020126982 10 43077074 43077085 12 + 0.664 0.184 -2.771
ENSG00000165731 E004 6.4152001 0.0027010028 0.0191092330 0.049576660 10 43077086 43077331 246 + 1.026 0.718 -1.187
ENSG00000165731 E005 4.3279024 0.0634463585 0.7576400744 0.842385026 10 43100459 43100483 25 + 0.764 0.693 -0.291
ENSG00000165731 E006 15.0285862 0.0013508828 0.4028887406 0.546685647 10 43100484 43100722 239 + 1.153 1.199 0.161
ENSG00000165731 E007 0.9117310 0.1339284021 0.3649290108 0.509264598 10 43102302 43102322 21 + 0.146 0.316 1.417
ENSG00000165731 E008 0.4031496 0.0246087599 0.3088606235 0.451125012 10 43102323 43102341 19 + 0.000 0.185 11.071
ENSG00000165731 E009 13.3096045 0.0018293770 0.0127233279 0.035261364 10 43102342 43102470 129 + 0.974 1.192 0.788
ENSG00000165731 E010 11.7758191 0.0028080540 0.0225018232 0.056724500 10 43102471 43102629 159 + 0.935 1.145 0.766
ENSG00000165731 E011 3.2010492 0.0050769278 0.0005283498 0.002265550 10 43102630 43102839 210 + 0.146 0.740 3.485
ENSG00000165731 E012 0.1451727 0.0438792553 0.3521571439   10 43104753 43104951 199 + 0.146 0.000 -11.547
ENSG00000165731 E013 7.7602394 0.0029157375 0.8458918927 0.904176271 10 43104952 43105193 242 + 0.915 0.911 -0.015
ENSG00000165731 E014 6.9675801 0.0024414693 0.2032769501 0.330307877 10 43106376 43106571 196 + 0.793 0.924 0.505
ENSG00000165731 E015 6.1549368 0.0027515661 0.1813867814 0.302983544 10 43109031 43109206 176 + 0.733 0.881 0.582
ENSG00000165731 E016 3.3965875 0.0065961344 0.2983864408 0.439824284 10 43109207 43109230 24 + 0.532 0.672 0.620
ENSG00000165731 E017 11.3176828 0.0015454906 0.6532221653 0.763757493 10 43111207 43111465 259 + 1.115 1.043 -0.261
ENSG00000165731 E018 11.9722667 0.0260342079 0.8557623954 0.910805884 10 43112099 43112224 126 + 1.128 1.074 -0.196
ENSG00000165731 E019 14.7640323 0.0238768771 0.2698253447 0.408385412 10 43112853 43112963 111 + 1.271 1.120 -0.536
ENSG00000165731 E020 17.2132089 0.0080616872 0.4367799849 0.578813910 10 43113556 43113675 120 + 1.298 1.200 -0.344
ENSG00000165731 E021 1.0373134 0.0125920360 0.0009152749 0.003667973 10 43113676 43114479 804 + 0.581 0.000 -14.355
ENSG00000165731 E022 25.4474327 0.0057247579 0.9805576450 0.991818854 10 43114480 43114736 257 + 1.413 1.382 -0.105
ENSG00000165731 E023 18.2093299 0.0054785124 0.8373590650 0.898329810 10 43116584 43116731 148 + 1.271 1.259 -0.040
ENSG00000165731 E024 16.6581604 0.0106064402 0.5920622864 0.714691661 10 43118373 43118480 108 + 1.200 1.224 0.085
ENSG00000165731 E025 22.8129983 0.0009155435 0.3259380893 0.469388014 10 43119531 43119745 215 + 1.323 1.365 0.147
ENSG00000165731 E026 19.3031437 0.0012324401 0.6452147399 0.757364399 10 43120081 43120203 123 + 1.289 1.295 0.023
ENSG00000165731 E027 0.3697384 0.0249721203 0.3094277317 0.451719995 10 43120204 43120912 709 + 0.000 0.184 11.069
ENSG00000165731 E028 14.6938968 0.0063635276 0.9686261337 0.984364049 10 43121946 43122016 71 + 1.200 1.175 -0.087
ENSG00000165731 E029 20.3416200 0.0009603331 0.0664559498 0.137481631 10 43123671 43123808 138 + 1.231 1.345 0.398
ENSG00000165731 E030 17.8402406 0.0090820096 0.0231141512 0.057985707 10 43124883 43124982 100 + 1.128 1.313 0.652
ENSG00000165731 E031 17.2167561 0.0011617741 0.3472700889 0.491467220 10 43126575 43126722 148 + 1.211 1.259 0.172
ENSG00000165731 E032 22.9086853 0.0008224314 0.0148282304 0.040105129 10 43126723 43128111 1389 + 1.251 1.403 0.529
ENSG00000165731 E033 6.2797831 0.0923375912 0.0163086671 0.043409504 10 43128112 43128285 174 + 1.087 0.619 -1.828
ENSG00000165731 E034 25.7687257 0.0690423729 0.0007614805 0.003120453 10 43128286 43129517 1232 + 1.650 1.183 -1.616
ENSG00000165731 E035 22.1518402 0.0032800120 0.2047784023 0.332114822 10 43129518 43130351 834 + 1.419 1.296 -0.429